The invention discloses a
calprotectin chemiluminiscence
immunoassay kit and a preparation method thereof, belongs to the field of in-vitro detection, and solves the problems of low sensitivity, highcost, poor accuracy, long
operation time, low
automation degree and incapability of quantification of an existing kit. The
chemiluminescence immunoassay kit comprises
streptavidin magnetic particles,a
chemiluminescence marker labeled
calprotectin antibody, a
coupling marker labeled
calprotectin antibody, a
chemiluminescence substrate solution and a calprotectin calibrator, wherein the concentration of the magnetic beads is 0.02-1%, and the particle size is 1.0-3.0 microns, the
molar ratio of the calprotectin
antibody to the chemiluminiscence marker is 1: (3-20), the
molar ratio of the calprotectin antibody to the
coupling marker is 1: (5-20), the chemiluminescence marker is acridinium ester,
luminol, isoluminol or terpyridyl
ruthenium. And the
coupling marker is
biotin. The method is highin sensitivity, high in accuracy, strong in specificity, high in automatic measurement degree, low in cost, small in error, short in measurement time and wide in application range.