Calprotectin detection kit and detection method

Abstract

The invention relates to a calprotectin detection kit and a detection method. The kit mainly comprises a microwell plate coated with calprotectin antibodies, a calprotectin calibrator, calprotectin antibodies labeled by horseradish peroxidase, an extraction buffer solution, a sample diluent, a substrate solution A (luminol solution), a substrate solution B (hydrogen peroxide solution) and a concentrated washing solution. According to the invention, a double-antibody sandwich immunodetection method is adopted; the microporous plate comprises an immobilized specific antibody; a sample and an enzyme-labeled antibody are added into a reaction hole of an enzyme-labeled plate, and a solid-phase immune complex containing a specific antibody-antigen-enzyme-labeled antibody is formed after a heat preservation reaction; the uncombined enzyme-labeled antibody is removed through washing, and the kit is suitable for large-batch detection; the kit is easy to operate, high in sensitivity, good in specificity, rapid and effective in detection and low in cost and has the application and popularization value.

Description

Technical field: [0001] The invention relates to the technical field of medical testing, in particular to a calprotectin detection kit. Background technique: [0002] Inflammatory bowel disease (IBD) is a group of chronic nonspecific inflammatory diseases of the intestinal tract with unclear etiology, including ulcerative colitis (UC) and Crohn's disease (CD). It is known that the inflammatory response caused by abnormal intestinal mucosal immune system plays an important role in the pathogenesis of IBD. Currently, the diagnosis of IBD mainly relies on medical history, clinical symptoms, colonoscopy, and pathological tissue biopsy. Although pathological tissue biopsy is the gold standard for diagnosis, it often delays diagnosis and treatment because of its pain, high cost, and inconvenient repeated examination. [0003] Calprotectin is a protein that binds calcium and zinc with a relative molecular mass of 36×10 3 , derived from neutrophils and activated macrophages, has ...

AI Technical Summary

Problems solved by technology

[0006] Related calprotectin detection kits are disclosed in the prior art, such as a human serum calprotectin detection kit and its preparation method with the patent number CN110161254A, and a fast and efficient calcium Protection protein detection kit and preparation method thereof, but the production cost of this type of calprotectin detection kit is high, the sensitivity is low, and the accuracy of test results is low

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