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Rapid and efficient calprotectin detection kit and preparation method thereof

A technology for detecting kits and calprotectin, applied in the field of calprotectin detection kits and its preparation, can solve problems such as inability to detect on-site, low sensitivity, long test time, etc., achieve sensitive detection, improve detection sensitivity, and improve sensitivity and stability effects

Inactive Publication Date: 2019-08-16
芜湖森爱驰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ELISA method is widely used in hospital laboratories because of its quantitative accuracy. However, ELISA needs to wash the plate, incubate, add enzymes and substrates, and the temperature environment is strict. The operation is cumbersome and cannot be detected on-site. Fast, but because the colloidal gold particles and the labeled protein are combined by electrostatic interaction, the combination is unstable and easily disturbed by the environment, the sensitivity is low, and quantitative detection cannot be performed; therefore, it is urgent to establish a fast, convenient and economical calcium Method for Quantitative Detection of Protectin

Method used

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  • Rapid and efficient calprotectin detection kit and preparation method thereof
  • Rapid and efficient calprotectin detection kit and preparation method thereof
  • Rapid and efficient calprotectin detection kit and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] A rapid and efficient calprotectin detection kit, including independent reagents R1 and R2, the reagent R1 includes: phosphate buffer 100mmol / L; sodium chloride 13g / L; Triton X-1006g / L; Bovine serum albumin 8g / L; ethylene glycol polyoxyethylene ether 90g / L; sodium azide 5g / L; and the pH value of the reagent R1 is 6.3-7.5;

[0038] Described reagent R2 comprises: phosphate buffer saline 100mmol / L; Anti-human calprotectin antibody coated latex particle 18mg / L; Blocking agent 20mg / L (comprising gelatin 2wt%, NH 2 -PEG3wt% and chitosan 3wt%, phosphate buffer saline 92wt%, wherein the concentration of phosphate buffer saline is 100mmol / L); Sodium chloride 5g / L; Bovine serum albumin 5g / L; Sodium azide 6g / L and the pH value of the reagent R2 is 7-8, wherein the concentration of the components in the reagent R2 is the final concentration of the components in the reagent.

[0039] The preparation method of described rapid and efficient calprotectin detection kit, the steps are ...

Embodiment 2

[0045] A calprotectin detection kit, comprising mutually independent reagent R1 and reagent R2, said reagent R1 comprises: phosphate buffer saline 80mmol / L; sodium chloride 12g / L; Triton X-100 5g / L; bovine serum Protein 6g / L; ethylene glycol polyoxyethylene ether 85g / L; sodium azide 3g / L; and the pH value of the reagent R1 is 6.3-7.5;

[0046] The reagent R2 includes: phosphate buffer saline 90mmol / L; anti-human calprotectin antibody coated latex particles 20mg / L; blocking agent 50mg / L (including gelatin 1wt%, NH 2 -PEG4wt% and chitosan 5wt%, phosphate buffer saline 90wt%, wherein the concentration of phosphate buffer saline is 100mmol / L); Sodium chloride 8g / L; Bovine serum albumin 6g / L; Sodium azide 5g / L and the pH value of the reagent R2 is 7-8, wherein the concentration of the components in the reagent R2 is the final concentration of the components in the reagent.

[0047] The preparation method of described rapid and efficient calprotectin detection kit, the steps are as...

Embodiment 3

[0053] A calprotectin detection kit, comprising mutually independent reagent R1 and reagent R2, said reagent R1 comprises: phosphate buffer saline 120mmol / L; sodium chloride 15g / L; Tween-20 8g / L; bovine serum albumin 10g / L; ethylene glycol polyoxyethylene ether 96g / L; p-hydroxybenzoic acid 6g / L; and the pH value of the reagent R1 is 6.3-7.5;

[0054] The reagent R2 includes: phosphate buffer saline 110mmol / L; anti-human calprotectin antibody coated latex particles 25mg / L; blocking agent 40mg / L (including gelatin 3wt%, NH 2 -PEG4wt% and chitosan 1wt%, phosphate buffer saline 92wt%, wherein the concentration of phosphate buffer saline is 100mmol / L); Sodium chloride 6g / L; Bovine serum albumin 5g / L; Parahydroxybenzoic acid 6g / L L; and the pH value of the reagent R2 is 7-8, wherein the component concentration in the reagent R2 is the final concentration of the component in the reagent.

[0055] The preparation method of described rapid and efficient calprotectin detection kit, the...

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Abstract

The invention relates to the technical field of biological detection, in particular to a rapid and efficient calprotectin detection kit and a preparation method thereof. The rapid and efficient calprotectin detection kit comprises a reagent R1 and a reagent R2 which are mutually independent, and is characterized in that: the reagent R1 comprises: 50-150mmol / L of phosphate buffer solution; 11-15g / Lof sodium chloride; 3-11g / L of surfactant; 5-12g / L of bovine serum albumin; 80-100g / L of polyethylene glycol; 3-6g / L of preservative; the pH value of the reagent R1 is 6.3-7.5; the reagent R2 includes: 80-120mmol / L of phosphate buffer solution; 15-30mg / L of latex particles coated with anti-human calprotectin antibody; 20-50mg / L of sealing agent; 5-8g / L of sodium chloride; 3-8g / L of bovine serum albumin; 4-6g / L of preservative; and the pH value of the reagent R2 is 7-8, wherein the concentration of the component in the reagent R2 is the final concentration of the component in the reagent. Thecalprotectin detection kit provided by the invention is capable of accurately, rapidly and sensitively detecting calprotectin in human excrement and blood.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a fast and efficient calprotectin detection kit and a preparation method thereof. Background technique [0002] Calprotectin is a calcium and zinc-binding protein with a molecular weight of 36 kD, which is composed of two heavy chains with a molecular weight of 14 kD and a light chain with a molecular weight of 8 kD. It can combine two Ca2+, so it has the characteristics of heat resistance and enhanced hydrolysis. Calprotectin, an important protein in the cytoplasm of neutrophils and activated macrophages, is increased in many inflammatory conditions and serves as a marker of acute inflammation. [0003] Although calprotectin has been found in many places in human organisms, including: serum, saliva, cerebrospinal fluid and urine. However, it is easy to detect in feces, and its composition is stable. It can exist stably in stool for about 7 days at room temperature...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893
Inventor 王晓波朱雪姣朱海峰
Owner 芜湖森爱驰生物科技有限公司
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