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Kit for quickly detecting calprotectin

A technology of calprotectin and bovine serum albumin, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of low sensitivity, long detection time, inconvenient, etc., and achieve the effect of high sensitivity, low cost, and strong specificity

Inactive Publication Date: 2017-05-31
SHENZHEN HUISONG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ELISA method is widely used in hospital laboratories because of its quantitative accuracy, but the detection time is long and inconvenient; although the colloidal gold method is convenient and fast, it is a qualitative method with low sensitivity; therefore, there is an urgent need to establish a clinical method. A fast, convenient and economical method for the quantitative detection of calprotectin

Method used

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  • Kit for quickly detecting calprotectin
  • Kit for quickly detecting calprotectin
  • Kit for quickly detecting calprotectin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] This embodiment provides a kit, including reagent R1, reagent R2 and calibrator.

[0078] Described reagent R1 comprises each component of following concentration:

[0079] Trishydroxymethylaminomethane 25mmol / L

[0080] Sodium chloride 8.5g / L

[0081] Triton X-100 5g / L

[0082] Bovine serum albumin 5g / L

[0083] Ethylene glycol polyoxyethylene ether 50g / L

[0084] Sodium azide 4g / L

[0085] The pH value of the reagent R1 is 7.0.

[0086] The reagent R1 is prepared by the following method:

[0087] Accurately weigh 3.03g tris, 8.5g sodium chloride, 5g Triton X-100 (Aladdin, article number: T109026, lot number: G1604036), 5g bovine serum albumin (Proliant, article number: 68100, lot number: BB52650101 ), 50g of ethylene glycol polyoxyethylene ether, and 4g of sodium azide were dissolved in 900ml of water, stirred until completely dissolved, adjusted to pH=7.0 with hydrochloric acid, and then adjusted to 1000ml, and stored for later use.

[0088] Described reagent...

Embodiment 2

[0101] This embodiment provides a kit, including reagent R1, reagent R2 and calibrator.

[0102] Described reagent R1 comprises each component of following concentration:

[0103] Phosphate 80mmol / L

[0104] Sodium chloride 8.5g / L

[0105] Triton X-100 10g / L

[0106] Bovine serum albumin 6g / L

[0107] Ethylene glycol polyoxyethylene ether 50g / L

[0108] Sodium azide 5g / L

[0109] The pH value of the reagent R1 is 7.5.

[0110]The reagent R1 is prepared by the following method: accurately weigh 28.65g disodium hydrogen phosphate dodecahydrate, 10.89g potassium dihydrogen phosphate, 8.5g sodium chloride, 10g Triton X-100 (Aladdin, article number: T109026 , batch number: G1604036), 6g bovine serum albumin (Proliant, article number: 68100, batch number: BB52650101), 50g ethylene glycol polyoxyethylene ether, 5g sodium azide were dissolved in 900ml of water, stirred until completely dissolved, then adjusted the pH with hydrochloric acid =7.5, then dilute to 1000ml, and save ...

Embodiment 3

[0123] This embodiment provides a kit, including reagent R1, reagent R2 and calibrator.

[0124] Described reagent R1 comprises each component of following concentration:

[0125]

[0126] The pH value of the reagent R1 is 7.0.

[0127] The reagent R1 is prepared by the following method:

[0128] Accurately weigh 1.56g sodium dihydrogen phosphate dihydrate, 3.58g disodium hydrogen phosphate dodecahydrate, 8.5g sodium chloride, 4.0g Tween-20, 5g bovine serum albumin (Proliant, article number: 68100, batch number: BB52650101 ), 4g of sodium azide were dissolved in 900ml of water, stirred until completely dissolved, adjusted to pH=7.0 with hydrochloric acid, then adjusted to 1000ml, and stored for later use.

[0129] Described reagent R2 comprises each component of following concentration:

[0130]

[0131] Reagent R2 has a pH of 7.5.

[0132] The reagent R2 is prepared by the following method:

[0133] Take 5ml of polystyrene latex (Millipore, particle size 250nm, 3% ...

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Abstract

The invention provides a kit. The kit comprises the following components of a polymer-containing buffer solution, and a polystyrene latex solution combined with anti-human calprotectin antigen. When the kit is used for detecting calprotectin, the kit has the advantages that in the use process, the kit is only added in two time periods, and the operation is very convenient and rapid; the sensitivity is high, and the low-level calprotectin can be detected; the specificity is strong, the repeatability is good, and the cost is low; the anti-interference ability is strong, and even if the detection sample contains a certain concentration of disruptors, such as hemoglobin, bilirubin, Vc and triglyceride, the influence to the detection result of the kit is little; the kit is suitable for quantitatively detecting the single part and the quick and instantaneous result, and the clinical application value is higher.

Description

technical field [0001] The invention relates to the field of medical immune in vitro diagnosis, in particular to a calprotectin detection kit. Background technique [0002] Calprotectin is a calcium and zinc binding protein with a molecular weight of 36kD. It consists of two heavy chains with a molecular weight of 14kD and a light chain with a molecular weight of 8kD, which are covalently linked calcium-binding protein heterotrimers. Each chain can bind two Ca 2+ , which has the characteristics of heat resistance and enhanced hydrolysis. [0003] Calprotectin is mainly derived from neutrophils and monocytes, and has a variety of biological functions. In vitro studies have shown that it has antibacterial properties, and its antibacterial effect can be compared with antibiotics. Calprotectin, an important protein in the cytoplasm of neutrophils and activated macrophages, is increased in many inflammatory conditions and serves as a marker of acute inflammation. [0004] Alt...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/577
CPCG01N33/54313G01N33/577
Inventor 李印军杨永崧吕典
Owner SHENZHEN HUISONG TECH DEV
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