Kit for quickly detecting calprotectin
A technology of calprotectin and bovine serum albumin, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of low sensitivity, long detection time, inconvenient, etc., and achieve the effect of high sensitivity, low cost, and strong specificity
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Embodiment 1
[0077] This embodiment provides a kit, including reagent R1, reagent R2 and calibrator.
[0078] Described reagent R1 comprises each component of following concentration:
[0079] Trishydroxymethylaminomethane 25mmol / L
[0080] Sodium chloride 8.5g / L
[0081] Triton X-100 5g / L
[0082] Bovine serum albumin 5g / L
[0083] Ethylene glycol polyoxyethylene ether 50g / L
[0085] The pH value of the reagent R1 is 7.0.
[0086] The reagent R1 is prepared by the following method:
[0087] Accurately weigh 3.03g tris, 8.5g sodium chloride, 5g Triton X-100 (Aladdin, article number: T109026, lot number: G1604036), 5g bovine serum albumin (Proliant, article number: 68100, lot number: BB52650101 ), 50g of ethylene glycol polyoxyethylene ether, and 4g of sodium azide were dissolved in 900ml of water, stirred until completely dissolved, adjusted to pH=7.0 with hydrochloric acid, and then adjusted to 1000ml, and stored for later use.
[0088] Described reagent...
Embodiment 2
[0101] This embodiment provides a kit, including reagent R1, reagent R2 and calibrator.
[0102] Described reagent R1 comprises each component of following concentration:
[0103] Phosphate 80mmol / L
[0104] Sodium chloride 8.5g / L
[0105] Triton X-100 10g / L
[0106] Bovine serum albumin 6g / L
[0107] Ethylene glycol polyoxyethylene ether 50g / L
[0108] Sodium azide 5g / L
[0109] The pH value of the reagent R1 is 7.5.
[0110]The reagent R1 is prepared by the following method: accurately weigh 28.65g disodium hydrogen phosphate dodecahydrate, 10.89g potassium dihydrogen phosphate, 8.5g sodium chloride, 10g Triton X-100 (Aladdin, article number: T109026 , batch number: G1604036), 6g bovine serum albumin (Proliant, article number: 68100, batch number: BB52650101), 50g ethylene glycol polyoxyethylene ether, 5g sodium azide were dissolved in 900ml of water, stirred until completely dissolved, then adjusted the pH with hydrochloric acid =7.5, then dilute to 1000ml, and save ...
Embodiment 3
[0123] This embodiment provides a kit, including reagent R1, reagent R2 and calibrator.
[0124] Described reagent R1 comprises each component of following concentration:
[0125]
[0126] The pH value of the reagent R1 is 7.0.
[0127] The reagent R1 is prepared by the following method:
[0128] Accurately weigh 1.56g sodium dihydrogen phosphate dihydrate, 3.58g disodium hydrogen phosphate dodecahydrate, 8.5g sodium chloride, 4.0g Tween-20, 5g bovine serum albumin (Proliant, article number: 68100, batch number: BB52650101 ), 4g of sodium azide were dissolved in 900ml of water, stirred until completely dissolved, adjusted to pH=7.0 with hydrochloric acid, then adjusted to 1000ml, and stored for later use.
[0129] Described reagent R2 comprises each component of following concentration:
[0130]
[0131] Reagent R2 has a pH of 7.5.
[0132] The reagent R2 is prepared by the following method:
[0133] Take 5ml of polystyrene latex (Millipore, particle size 250nm, 3% ...
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