756results about How to "High clinical application value" patented technology

Disclosed are competition assay methods for reliably detecting the presence and / or quantitation of small molecules (e.g., metabolites) and peptides / proteins in a sample by the use of capture agents specific for immobilized small molecules and / or peptides / proteins. Arrays comprising these small molecules and / or peptides / proteins are also provided.

Disclosed are reagents and methods for reliably detecting the presence and measuring the amount of proteins, including proteins with various post-translational modifications (phosphorylation, glycosylation, methylation, acetylation, etc.) in a sample by the use of one or more capture agents that recognize and interact with recognition sequences uniquely characteristic of a protein or a set of proteins (Proteome Epitope Tags, or PETs) in the sample. Arrays comprising these capture agents or PETs are also provided.

The invention discloses a latex intensified immunological turbidimetry kit for detecting cystatin C in serum or plasma. The kit adopts antibodies which resist the cystatin C coated by latex to generate chain reaction with cystatin C to be detected, latex gathers layer by layer by immunoreaction to form insoluble immune particle complexes, the particle size of the latex has a change to cause the turbidity to have obvious change, light transmission strength (570 mm) or light scattering strength is used to detect the change, and the concentration of the traget detection object cystatin C in a sample is got from a standard curve made by a cystatin C standard product with the known concentration. The kit comprises a reagent R1, a reagent R2 and the cystatin C standard product. Detection of the cystatin C by the reagents is carried out by latex agglomeration tests, as well as the reagents are ideal endogenous markers for detecting glomerular filtration rate GFR and are more sensitive than and have earlier period than creatinine. Using the method, the sensitivity is high, and the cystatin C with extremely low level can be ensured to be detected, thus the invention has high clinical application value.

The invention discloses a moist antibacterial hydrogel dressing for curing diabetic foot ulcer, and relates to the technical field of medical instruments and preparation thereof. The dressing is prepared by the following steps: dissolving ionic compounds in sodium alga acid water solution, and obtaining nano-silver sodium alga acid solution through adding reducing agent; adopting the impregnation method, placing the nano-silver sodium alga acid solution in a bacterial cellulose membrane, so as to obtain bacterial cellulose / nano-silver sodium alga acid composite membrane through freeze drying; and immersing the composite membrane in calcium chloride water solution, and obtaining the moist antibacterial hydrogel dressing for curing diabetic foot ulcer. The dressing is a composite hydrogel membrane including bacterial cellulose hydrogel and nano-silver contained calcium alginatehydrogel. The preparation is convenient to operate and low in cost, and the moist antibacterial hydrogel dressing prepared for curing diabetic foot ulcer has the performances of sterilizing, preserving moisture, absorbing wound percolate, quickening wound healing and the like, and is applied to curing wound traumas of diabetic foot ulcer, venous ulcer, pressure sores and donor site wounds at the lower limbs.

The invention provides a sustained-release temperature-sensitive blood vessel embolic gel used for treating tumor. The sustained-release blood vessel embolic gel is prepared by entrapping a medicine by using a pharmaceutically acceptable carrier. The medicine is an anti-tumor medicine, and the pharmaceutically acceptable carrier comprises a gel prepared from poloxamer polymer, polyvinyl pyrrolidone, and the like or a composition thereof. The polymer material accounts for 5-65% of a gel mass. The particle size of the gel is in a range of 10nm to 150mum. The embolic agent is liquid gel under normal temperature, and can be used for direct injection through catheter. After injection into body, with the increase of temperature, the liquid gel is rapidly solidified into gel. Also, according to requirements, different medicines can be entrapped, and embolism and medication dual effect can be achieved through local sustained-release. Therefore, the gel provided by the invention can be used as an embolic agent for endovascular treatment, and can be used in various benign and malignant tumor transcatheter arterial chemoembolizations. The preparation method provided by the invention is simple, and is suitable for industrialized productions.

A preparation technology of amlodipine besylate is disclosed in the present invention, the technology is with amlodipine besylate, filler, disintegrating agent, lubricant etc. as main components. By using grinding and sieving; reasonably controlling particle water; repeatedly feeling fluidized bed granulating technology parameter, controlling spraying speed, spraying pressure and air quantity, under the situation of non-affecting tablet content and hardness, increasing the solubility of product greatly, thereby promoting the internal quality and curative effect of the product.

The invention discloses a method for achieving optical surgical navigation surgical instrument calibration through a calibrating device. The method comprises the steps of 1) establishing a calibrating device coordinate system; 2) calculating a transfer matrix from a surgical navigation coordinate system to the calibrating device coordinate system; 3) establishing a surgical instrument coordinate system and calculating a transfer matrix between the surgical instrument coordinate system and the surgical navigation coordinate system; 4) calculating a transfer matrix from the calibrating device coordinate system to the surgical instrument coordinate system; 5) calculating the coordinates and orientation of a tip of a surgical instrument in the surgical instrument coordinate system. The method reduces operating complexity, reduces calibrating time, is suitable for field calibration and has higher clinical application value.

The invention relates to a segmentation method of medical images, and specifically relates to a segmentation method of viscera and internal blood vessels thereof in a surgical planning system. The method designs a segmentation method of images of liver parenchyma, portal veins and hepatic veins based on minimal supervised classification of the three-dimensional CT images of the viscera and internal tubular tissues thereof. The method applies methods of statistics and spatial information, introduces high credible points, and obtains segmentation results based on arrival time of fast march of grey level. The method assumes that spatially adjacent points belong to a same organ, and performs segmentation calculation on the images by using a classification algorithm based on the above assumption, wherein the segmentation calculation comprises the steps of: acquiring estimated values of parameters of a Gaussian mixture distribution model; selecting the high credible points; and calculating the mean value of the grey level of the images and calculating the arrival time by using a fast marching algorithm to obtain arrival time images of three tissue classifications. According to the invention, good robustness and good anti-noise interference capability are obtained, accumulated errors are eliminated effectively, classification accuracy is improved, and great application value in clinical treatment is achieved.

The invention discloses an emulsion multi-dimensional rapid preparation microfluidic device, and discloses a compound medicine microcarrier with a core-shell structure, and applications thereof. The compound medicine microcarrier with the core-shell structure is characterized by being prepared through taking W / O / W or O / W / O double emulsion as templates by adopting a microfluidic method, a core and a shell are respectively made of two degradable polymer materials with biocompatibility and are respectively hydrophilic and hydrophobic. The hydrophilic part can be loaded with various hydrophilic medicines, the hydrophobic part can be loaded with various hydrophobic medicines; along with the degradation of the core-shell material, the hydrophilic and hydrophobic drugs loaded internally can be sustainably released, and the purpose that the hydrophilic and hydrophobic medicines are simultaneously delivered and synergically slow-released can be achieved. In addition, the release rate of the medicine can be controlled by controlling the thickness of the shell layer. The microcapsule with the core-shell structure has the characteristics that the preparation method is low in cost, easy to operate and convenient to produce on large scale, and the like, the prepared medicine microcarrier has good biocompatibility, is high in medicine encapsulation rate, and good in controllability.

The invention discloses a kit for processing an antigen-antibody immune complex in a serum or plasma sample. The kit is prepared from a meta-acid buffer solution serving as a solvent, high-concentration salt ions, an ionic surfactant, a nonionic surfactant, a reducing agent, urea, trehalose and a defoaming agent. The kit is suitable for processing the serum or plasma sample which is suspected to generate the antigen-antibody immune complex to further influence the antigen or antibody detection result, and the processed serum or plasma sample is suitable for the sample detection, such as enzyme-linked immunoassay and chemiluminescence immunoassay, while the sample which does not generate the immune complex does not affect the normal detection result after being processed via the conditioning fluid of the kit. The kit disclosed by the invention can effectively dissociate the formed antigen-antibody immune complex in the sample, greatly improves the detection sensitivity of the sample, and has a very high clinical application value.

The invention discloses a prognosis marker for lung cancer, a method for anticipating lung cancer prognosis by using the marker and application of the marker. The marker comprises the following gene combinations: RP11-89K21.1, CDH17, GRIA1, CENPF, AP000438.2, HMMR, RP11-434D9.1, ADH1B, TPX2 and OR7E47P. The prognosis marker for lung cancer overcomes the defect that the difference between lung cancer treatment reaction and prognosis is big, resulting in inaccuracy and inconvenience in prognosis, and the prognosis marker for lung cancer rapidly, accurately and conveniently anticipates lung cancer prognosis through the marker and detection of the marker, realizes classification and stratification of high and low lung cancer hazards on the molecular level, and remarkably separates patients with high risk from patients with low risk, thereby guiding individualized treatment, having higher clinical application value and saving medical treatment cost.

Disclosed are methods for reliably detecting the presence of proteins, especially proteins with various post-translational modifications (phosphorylation, glycosylation, methylation, acetylation, etc.) in a sample by the use of one or more capture agents that recognize and interact with recognition sequences uniquely characteristic of a set of proteins (Proteome Epitope Tags, or PETs) in the sample. Arrays comprising these capture agents or PETs are also provided.

The invention belongs to the field of diagnostic reagents, and relates to a diagnostic reagent containing mycobacterium tuberculosis Rv1985c protein and a kit. The diagnostic reagent and the kit can perform rapid diagnosis on whether the blood or the body fluid of an experimenter is infected by the mycobacterium tuberculosis in the time periods from 15 minutes of an immune colloidal gold to 5 hours of ELISA. When the diagnostic reagent is used for the clinically-proved abortive tuberculosis, a Rv1985c antigen is used singly to detect that the sensitivity reaches 59 percent and the specificityreaches 96 percent; and when used together with other diagnostic antigens (such as a control antigen LAM / 38kDa), the diagnostic reagent can further improve the diagnostic sensitivity to 75 percent and has high clinical application values. The detection with the diagnostic reagent only needs a single blood serum sample, is simple and quick in test, needs no specialized laboratory equipment, is lowin cost, offers test results in the same day, and is very suitable for the detection of tuberculosis infection in extensive rural hospitals of villages and towns and under the condition of battlegrounds.

The invention relates to the field of medical immonological in vitro diagnosis and in particular relates to a retinol binding protein assay kit based on latex particle coating. The assay kit comprises a reagent R1, a reagent R2 and a calibrator, wherein the reagent R1 is a Tris-HCl buffer system, and comprises a Tris-HCl buffer solution, a polymer and ethylene diamine tetraacetic acid; the reagent R2 comprises goat anti human retinol binding protein polyclonal antibody coated polystyrene latex particle sensitized granules and a phosphate buffer solution; and the reference calibrator comprises bovine serum matrix, as well as 0.2-2.2% of antiseptic and 1-10% of stabilizer according to the volume percentage of the bovine serum matrix. Compared with the prior art, the assay kit can be used for assistant diagnosis of kidney diseases and assistant diagnosis of mal-nutrition, and has high clinical application value.

A medical living body observation apparatus, an intraoral photography system and a medical treatment appliance which can be used conveniently by a general user at home as well as a technician, e.g. a dentist or a physician. There is provided an irradiation means (2) comprising an exciting light emission section (2a) emitting an exciting light for causing a lesion to radiate fluorescence, and an illumination light emission section (2b) emitting a light for illuminating the periphery of the lesion. The irradiating means (2) can simultaneously irradiate the illumination light and the exciting light.

The invention is applied to the field of medical image segmentation technology, and provides a bronchial partition method and a system thereof. The bronchial partition method includes the following steps: a first segmenting step, segmenting and extracting a main area of the lung and the bronchus from an original lung image through three-dimensional growth algorithm; a bronchus enhancing step, carrying out multiple times of expansion operations and multiple times of corrosion operations to the main area of the lung and the bronchus; and a second segmenting step, extracting the bronchus from the main area of the lung and the bronchus after the multiple times of expansion operations and multiple times of corrosion operations. The bronchial partition method strengthens the bronchus through information of vascular so as to avoid effect of gray value change, and a segmenting result of the bronchus is enabled to be more stable and accurate.

The present invention provides a bladder pyeloscope, comprising a pyeloscope sheath, a pyeloscope body, a lithotomy forceps and clamp module, a lithotomy forceps and clamp passage pipe, a chippings passage pipe, an optical fiber passage pipe and an endoscopic imaging module. The end of the pyeloscope sheath is provided with a washing liquid passage opening. One ends of the lithotomy forceps and clamp passage pipe, the chippings passage pipe and the optical fiber passage pipe are respectively communicated with a pull rod inlet at the pyeloscope body, a chippings passage opening and an optical fiber inlet; the other ends are inserted into the pyeloscope sheath and are extended to the end of the pyeloscope sheath. The chippings passage opening is communicated with a chippings passage. The gap inside the pyeloscope sheath is a liquid washing passage which is communicated with the washing liquid passage opening. The lithotomy forceps and clamp module comprises a jaw and a pull rod which is put through the lithotomy forceps and clamp passage to be connected with a handle. The lithotomy forceps and clamp module is arranged outside the end of the lithotomy forceps and clamp passage pipe. One end of the endoscopic imaging module is connected with the pyeloscope body, and the other end is arranged at the end of the optical fiber passage pipe. The present invention has the functions of diagnosing, fixing stone, washing and absorbing chippings, taking the chippings, etc. and has important clinical application value.

The invention relates to a construction method of a stomach cancer image recognition model based on artificial intelligence. The image recognition model can accurately recognize a lesion site in a stomach cancer image.

The invention discloses a nimodipine micelle injection and a preparation method thereof. The nimodipine micelle injection is prepared by using active ingredients, i.e. nimodipine and phospholipid / bile salt. According to the nimodipine micelle injection disclosed by the invention, the solubility of the nimodipine is greatly increased, and no crystal precipitation exists when the nimodipine micelle injection is diluted; the biocompatibility of the phospholipid / bile salt is good, and organic solvents with great toxic side effects, such as ethyl alcohol, propylene glycol and the like, are not needed to for solubilizing so that the toxicity of the nimodipine micelle injection is lowered, the vascular stimulation is little, and the adverse reaction of the nimodipine micelle injection is reduced; and the nimodipine micelle injection can be directly used for intravenous injection and can also be used for instilling after the nimodipine micelle injection is diluted to various degrees, and thus, the clinical administration is greatly facilitated.

The invention discloses a vascular stenosis detection method and device. The method comprises the steps of acquiring a computed tomography angiography (CTA) image for a blood vessel to be detected; straightening and imaging the CTA image at a plurality of set angles to obtain a plurality of straightened images at a plurality of set angles; carrying out width measurement on the straightened imagesone by one, and outputting a width curve corresponding to the straightened image with the set angle; and performing plaque positioning screening on the width curve one by one, and determining a narrowarea of the blood vessel to be detected according to a plaque positioning screening result. By implementing the method and the equipment provided by the invention, the accuracy of vascular stenosis detection can be improved.

The invention provides a new route of administration for sirolimus, namely that sirolimus is prepared into an external preparation for treating skin diseases. The external preparation is a prescribed preparation or compound preparation containing sirolimus, and the dosage form can be any one of pharmaceutical skin external dosage forms including cream, ointment, gel, spray, coating agent and the like, and can be any one of new external dosage forms including solid lipid nanoparticles and the like. The research shows that the external preparation containing sirolimus can be used for treating multiple immune and inflammatory skin diseases including atopic dermatitis, eczema, dermatitis, lichen planus, psoriasis, vitiligo, rosacea, sarcoidosis and the like, and is good in curative effect, high in safety and free of skin irritation. The external preparation is expected to become an important pharmaceutical preparation for clinically treating skin diseases following corticosteroids.

Disclosed are methods for reliably detecting the presence of proteins, especially proteins with various post-translational modifications (phosphorylation, glycosylation, methylation, acetylation, etc.) in a sample by the use of one or more capture agents that recognize and interact with recognition sequences uniquely characteristic of a set of proteins (Proteome Epitope Tags, or PETs) in the sample. Arrays comprising these capture agents or PETs are also provided.

The invention relates to an anti-infection PMMA (polymethyl methacrylate) bone cement for composite chitosan quaternary ammonium salt. The composite bone cement is prepared by mixing HACC, PMMA and bone cement monomers, wherein the mass ratio of the HACC and the PMMA is 15-30%. The invention also provides a preparation method and applications for the composite bone cement. The invention has the advantages that the PMMA of the composite HACC can replace the current frequently-used PMMA antibiotic bead chain, can be applied to bone defect filling, infection prevention, treatment of chronic osteomyelitis and centrum reinforcement of osteoporosis, achieves the antimicrobial activity similar to the antibiotic bone cement, simultaneously avoids the generation of antibiotic resistance, can be used for patients with resistance to antibiotics, and has larger clinical application values; and appropriate reduction of elastic modulus is also beneficial to reducing the non-uniform stress distribution of a cured centrum and an adjacent centrum section, thus relieving the early degeneration of adjacent intervertebral disk and fracture of adjacent centrums.

The invention relates to a hepatitis B virus multi-drug resistant gene locus typing detection method and a kit thereof, wherein the detection method comprises the following steps that: DNA extraction is performed, wherein PCR amplification is applied to the abstracted DNA, the used PCR primers are two pairs of positive primers and negative primers; the PCR primers contain all correlative drug resistant locuses; amplified products of PCR are subjected to LDR amplification; the used probes are probes for correlation locus, which comprise a fluorescence labeling probe and a detection probe; a sequencing machine analyzes LDR products to judge the genotype of drug resistant locus; the multi-drug resistant gene locus is a drug resistant locus of lamivudine, ADV and ETV. The hepatitis B virus multi-drug resistant gene locus typing detection kit has reliable stable result, simple and quick operation; the kit can detect the drug resistant condition of various hepatitis B viruses, and supply clinical individuation medicine application instruction to hepatitis B virus patients. The kit can reduce the ineffectivity of drugs and administration times of the patients, thereby alleviating pains and economic burden of the patients.

The invention provides a kit for determination of apolipoprotein C2 by using immunoturbidimetry. A reagent R1 contains 10 to 500 mM of a buffer solution, 1 to 100 g / L of a surfactant, 5 to 50 g / L of an electrolyte, 2 to 100 g / L of a high-molecular accelerator accelerator, 1 to 50 g / L of a stabilizing agent and 1 to 10 g / L of an antiseptic; a reagent R2 comprises 10 to 500 mM of the buffer solution, 150 to 300 g / L of an anti-human APOC2 antibody, 5 to 50 g / L of the electrolyte, and 1 to 10 g / L of the antiseptic; and a calibrator comprises 10 to 500 mM of the buffer solution, 0 to 100 g / L of an APOC2 antigen, 1 to 50 g / L of the stabilizing agent, 1 to 10 g / L of the antiseptic and 0.01 to 10 g / L of an anti-oxidant. The kit provided by the invention is easy and fast to use, can satisfy clinical requirements for rapid high flux detection of a sample and has the advantages of obviously improved detection efficiency, small batch difference and stable reagents.

The invention provides a black phosphorus-based hydrogel near-infrared immune drug controlled-release system. The system comprises an agarose hydrogel carrier as well as black phosphorus nanosheets and anti-cancer immune drugs carried in the agarose hydrogel carrier. The black phosphorus-based hydrogel near-infrared immune drug controlled-release system has near-infrared response, and can realize transformation from a gel state to a sol state through near-infrared light irradiation, so that locally controllable drug release can be achieved, and tumor cells in focus positions can be effectively killed; the system has a controllable degradation characteristic, and has extremely high clinical value for cancer treatment. The invention also provides a preparation method of the black phosphorus-based hydrogel near-infrared immune drug controlled-release system.

The invention relates to a kit for rapidly detecting single nucleotide polymorphism (SNP) rs12979860 of an IL28B gene. By the kit, a specific primer is designed, the specific primer and template deoxyribonucleic acid (DNA) in a sample are subjected to an ordinary polymerase chain reaction (PCR), and the polymorphic forms (CC, CT and TT) of IL28B SNP rs12979860 of the sample is judged according to the result of the PCR, so that a result of therapeutic effect prediction is provided for the clinical antiviral therapy of hepatitis C patients. The kit is easy to operate, can detect the polymorphism quickly, is economic and effective, can be widely used for screening the therapeutic effect of the hepatitis C patients before antiviral therapy, is convenient for clinicians to formulate therapeutic schedules pertinently, has high clinical application value, and is particularly suitable for clinical promotion.

The invention relates to the technical field of in-vitro nucleic acid detection and particularly provides a primer group and kit for detecting polymorphic sites of aspirin-resistant related genes, andan application thereof. The primer group and the kit for detecting polymorphic sites of aspirin-resistant related genes and the kit can detect polymorphism of genes such as COX1, COX2, GPIIIa, PEAR1,P2Y1, GPIa and PAI-1; the sensitivity is high, so that genome DNA low to 0.1ng / muL can be accurately detected; the specificity is good, the genome DNA up to 300ng / muL can not generate non-specific amplification; the whole Fluorescence PCR detection process can be finished only within 90 minutes, and the detection result is intuitive and easy in interpretation.

The invention discloses a method for medical ultrasound three-dimensional imaging based on parallel computers. The method includes the following steps: S1. creating a task pool, namely, reading image data in accordance with user setting parameters and construct the task pool; S2. conduct partitioning to obtain sub-tasks, namely, adopting domain partition method to partition collected ultrasound image sequences into a plurality of sub-tasks in accordance with defined particle size, wherein each sub-task is an interpolation to complete adjacent images of S frames; S3. sub-tasks distribution, namely, distributing sub-tasks to different processors; S4. calculating sub-tasks, namely each processor executing sub-tasks coordinately and parallelly; S5. overall interpolation, namely conducting overall interpolation to sub-task results obtained by reconstruction of each processor to get a three-dimensional image; and S6. image display, namely displaying the reconstructed three-dimensional image. The method solves the time-consuming problem of the ultrasound three-dimensional imaging. Besides, the parallel computer environment is constructed by low-cost computers. Thus, the computing resources can be fully used and the cost is also low.

The invention discloses a kit for detecting alpha 1-acidoglycoprotein by using an immunity transmission turbidity method. A reagent R1 comprises 10-500 mM buffer liquid, a 0.5-50 g / L surfactant, a 5-50 g / L electrolyte, a 2-100 g / L high molecular accelerant, a 5-50 g / L stabilizing agent and a 1-10 g / L preservative; a reagent R2 comprises 10-500 mM buffer liquid, a 150-300 g / L anti-human alpha 1-acidoglycoprotein antibody, a 5-50 g / L electrolyte and a 1-10 g / L preservative; and a standard product comprises 10-500 mM buffer liquid, a 5-50 g / L alpha 1-acidoglycoprotein antigen, a 5-50 g / L stabilizing agent, a 1-10 g / L preservative and a 0.01-10 g / L antioxidant. The kit has the advantages of simplicity and fastness in use, remarkably improved detection efficiency and stable reagent, can satisfy the requirement of clinical and quick high throughout sample detection, and is suitable for clinical promotion.