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Joint detection kit for testing intestinal mucosa injury and preparation method of joint detection kit

A joint detection technology for intestinal mucosal injury, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to perform single-person detection in time, narrow detection linear range, and inaccurate detection results, etc., to achieve long-lasting luminous signals, Effect of improving detection sensitivity and improving linear range

Inactive Publication Date: 2018-10-02
常州领航量子生物医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzyme-linked immunosorbent method has many problems such as cumbersome operation, long detection time and inability to perform single-person detection in time; although the colloidal gold immunochromatography method is easy to operate and short detection time, it has low detection sensitivity and narrow detection linear range and other factors, the test results are inaccurate

Method used

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  • Joint detection kit for testing intestinal mucosa injury and preparation method of joint detection kit
  • Joint detection kit for testing intestinal mucosa injury and preparation method of joint detection kit
  • Joint detection kit for testing intestinal mucosa injury and preparation method of joint detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Prepare the mixture of quantum dot-labeled calprotectin antibody 1

[0035] (1) Take 1ml of quantum dot fluorescent microspheres (the concentration is 10mg / ml, the particle size is 180nm, CdSe / ZnS, the surface group is carboxyl group, the excitation wavelength is 410, the emission wavelength is 615, purchased from: Suzhou Weidu Biotechnology Co., Ltd. ), adding 16.5ml of labeling buffer, and ultrasonically mixing;

[0036] labeling buffer

[0037]

[0038](2) Add 0.5ml each of Activation Buffer 1 and Activator Buffer 2, shake and react at 37°C for 25 minutes, and ultrasonically disperse for 20 seconds every 4 minutes;

[0039] Activation buffer 1

[0040]

[0041] Activation buffer 2

[0042]

[0043] (3) Add 1.4 mg of Calprotectin Antibody 1 (purchased from: Medix Biochemica), ultrasonically mix, shake and react at 37°C for 1 hour and 20 minutes, and ultrasonically disperse for 25 seconds every 8 minutes during this period;

[0044] (4) Add 1.5ml blocki...

Embodiment 2

[0095] 1. Prepare the mixture of quantum dot-labeled calprotectin antibody 1

[0096] (1) Take 1ml of quantum dot fluorescent microspheres (the concentration is 10mg / ml, the particle size is 160nm, InP, the surface group is carboxyl, the excitation wavelength is 405, the emission wavelength is 605), add 15.5ml of labeling buffer, and mix well by ultrasonic ;

[0097] labeling buffer

[0098]

[0099] The quantum dot fluorescent microspheres can also be silicon, germanium, cadmium sulfide, cadmium telluride, zinc selenide, lead sulfide, lead selenide, indium arsenide and polystyrene microspheres, polymethacrylate microspheres, poly Compounding of ethylene toluene microspheres or silica microspheres;

[0100](2) Add 0.45ml each of Activation Buffer 1 and Activation Buffer 2, shake and react at 37°C for 30 minutes, and ultrasonically disperse for 30 seconds every 5 minutes during this period;

[0101] Activation buffer 1

[0102]

[0103]

[0104] Activation buffer 2...

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Abstract

The invention belongs to the field of biological detection and particularly relates to a joint detection kit for testing intestinal mucosa injury and a preparation method of the joint detection kit. Adetection line T1, a detection T2 and a quality control line are arranged on a detection pad; the detection line T1 is coated with a calprotectin monoclonal antibody; the detection line T2 is coatedwith a hemoglobin monoclonal antibody; the quality control line is coated with goat-anti-mouse or sheep-anti-rabbit IgG; a marking pad is coated with a mixture of the calprotectin monoclonal antibodymarked by a quantum dot and the hemoglobin monoclonal antibody marked by the quantum dot; the quantum dot is a mixture of two different granule particle sizes. The kit provided by the invention is capable of synchronously detecting calprotectin and fecal occult blood in excrement, capable of rapidly and conveniently judging mucosa injury degrees, and is high in detection sensitivity and good in specificity.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a combined detection kit for measuring intestinal mucosal damage and a preparation method thereof. Background technique [0002] Calprotectin belongs to the S-100 family and is a calcium-zinc binding protein with a relative molecular mass of 36kDa. It is a heterotrimer composed of two MRP-8 chains and one MRP-14 chain, mainly derived from Neutrophils are important markers of neutrophil renewal. [0003] The presence of calprotectin in feces is a hallmark of gastrointestinal proliferative disorders and intestinal inflammatory infectious diseases. In general, it is difficult to distinguish the discomfort caused by acute intestinal irritation from chronic infectious gastrointestinal diseases from the symptoms of the disease alone, which leads to unnecessary and excessive abuse of colonoscopy. A sign of a tract infection, and from this the extent of the infection can...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/577G01N33/533
CPCG01N33/533G01N33/558G01N33/577
Inventor 李小冬高省
Owner 常州领航量子生物医疗科技有限公司
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