Preparation method and application of bacillus methylotrophilus LW-6 bacterial agent for biological control of meloidogyne
A methylotrophic, bacillus-based technology, applied to chemicals for biological control, microbial-based methods, biocides, etc., can solve unsatisfactory effects, reduced quality of tobacco leaves, root-knot nematode hazards, etc. problems, to achieve the effects of strong environmental adaptability and environmental friendliness, strong fixed value and competitive ability, and simple and easy preparation method
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Embodiment 1
[0024] Preparation of a methylotrophic bacillus LW-6 bacterial agent for biological control of root-knot nematode:
[0025] (1) plate activation and optimal cultivation: the methylotrophic bacillus LW-6 stock slant is inoculated on the plate medium for activation, and the composition of the plate medium and its percentage by weight are: peptone 3.5%, yeast extract 1.3% , NaCl 1.5%, agar powder 5.7%, glucose 0.9%, sodium carbonate 0.1%, magnesium sulfate 0.1%, iron sulfate 0.1%, the balance is water, pH is 7.2~7.3, culture conditions are: at a temperature of 32 ℃ Under culture for 80h.
[0026] (2) Seed culture: inoculate the activated plate bacteria into the preferred liquid medium, and cultivate it for 80 hours at a temperature of 32° C. to obtain a fermented seed liquid; wherein the composition and weight percentage of the liquid medium are: peptone 3.5%, yeast extract 1.4%, NaCl 1.8%, glucose 00.9%, sodium carbonate 0.1%, magnesium sulfate 0.1%, iron sulfate 0.1%, the bala...
Embodiment 2
[0031] Indoor biological control effect of methylotrophic Bacillus LW-6 bacterial agent on root-knot nematode The isolation of root-knot nematode was carried out by the Baemann funnel method, and the second instar larvae of root-knot nematode were obtained. The isolated root-knot nematodes were added to a 500-fold dilution of the liquid preparation of methylotrophic Bacillus LW-6 bacteria, and the same amount of water was used as a control, and the nematodes were left at room temperature for 1 hour to observe the number of surviving and dead nematodes. The results showed that, in the control, the mortality rate of the second instar larvae of root-knot nematode was 4.41%, while the mortality rate of the second instar larvae of root-knot nematode treated with the 500-fold dilution of the liquid preparation of methylotrophic bacillus LW-6 reached 93%, so it can be seen that the liquid preparation with methylotrophic bacillus LW-6 as the active ingredient has a strong biological co...
Embodiment 3
[0033] Control effect of methylotrophic Bacillus LW-6 bacterial agent on tobacco root-knot nematode in field plots. The variety of flue-cured tobacco is Honghua Dajinyuan. Add 500ml of water to 1 gram of vegetative Bacillus sp. LW-6 to irrigate the roots, and use the clear water without the preparation as a control. 60 days after transplanting, the disease index of tobacco root-knot nematode disease was investigated by digging roots. During the transplanting period, the treatment group was repeatedly treated twice, and the control group was repeatedly treated with clean water. The disease index of tobacco root-knot nematode treated with the methylotrophic bacillus LW-6 bacterial agent was 20.3, while the disease index of the blank control without applying the preparation reached 69.5. It can be seen that the methylotrophic bacillus LW- The biological control effect of the preparation with 6 as the active ingredient on tobacco root-knot nematode disease is 70.79%.
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