Bacillus aerophilus and application of bacillus aerophilus in killing meloidogyne

A technology of Bacillus aerophilus, ZDXC-01, applied in the field of microorganisms, can solve the problems of rampant occurrence of secondary diseases, pollution, loss of resistance, etc., and achieve good application prospects, fast growth and strong stress resistance

Active Publication Date: 2017-09-15
CHINA KINGDOM AGRITECH QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the use of chemical nematicides can achieve immediate and expected results, they are widely used by everyone, but the long-term use of chemical agents has brought a series of side effects, such as environmental pollution caused by chemical residues, rampant occurrence of secondary diseases, and loss of resistance etc. are being gradually restricted
[0004] No Bacillus aerophilus ( Bacillus aerophilus ) can be used to control root-knot nematode damage to crops

Method used

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  • Bacillus aerophilus and application of bacillus aerophilus in killing meloidogyne

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Bacillus aerophilus ( Bacillus aerophilus ) Discovery and Identification of ZDXC-01

[0023] The present inventor isolates monoclonal bacterial strains from seawater by dilution coating method, and the specific separation method is: take 1mL seawater sample and dissolve in 99mL sterile water, vibrate to make it fully mix, promptly obtain 10 -2 seawater dilution. Take 1ml of the above seawater dilution to 9ml sterile water, shake well to obtain 10 -3 seawater dilution. Configure 10 in the same way -4 、10 -5 、10 -6 seawater dilution. take 10 -4 、10 -5 、10 -6 Put 100 μL of seawater dilution solution on the agar-added 2216E medium plate, spread the dilution evenly with a spreader, place the plate in an incubator at 28-30°C for 24 hours, and colonize different forms of bacteria on the medium on the Streak on blank 2216E medium plate, purify the obtained strains, and store them with respective numbers. Among them, the microscopic characteristics of a stra...

Embodiment 2

[0024] Embodiment 2 Preparation of anti-root-knot nematode fermented liquid

[0025] The fermentation process for preparing a large amount of Bacillus aerophilus ZDXC-01 is as follows:

[0026] (1) Streak the Bacillus aerophilus ZDXC-01 stored on the slant of the test tube on the 2216E medium or LB medium with agar for activation, and culture it in a constant temperature incubator at 28-30°C for 3 days;

[0027](2) Transplant the spores of Bacillus aerophilus ZDXC-01 in step (1) into 2216E (yeast extract 1g, peptone 5g, filtered seawater 1000mL, pH 7.2-7.4) or LB liquid medium (tryptone 10g , yeast extract 5g, sodium chloride 10g, distilled water 1000mL, pH adjusted to 7.2-7.4), shake culture at 28-30°C for 3 days to obtain seed liquid;

[0028] (3) Inoculate the seed liquid into the fermentation medium (corn flour 10g, soybean meal 10g, soluble starch 5g, potassium dihydrogen phosphate 1g, sodium chloride 5g, distilled water 1000 mL, pH 7.2- 7.4), shaking culture at 28-30°C...

Embodiment 3

[0029] Example 3 Indoor in vitro test against tomato root-knot nematode

[0030] 1.1 Preparation of test agents

[0031] The anti-root-knot nematode fermentation broth of Bacillus aerophilus ZDXC-01 freshly prepared according to Example 2.

[0032] 1.2 Preparation of test nematodes

[0033] The nematodes tested were obtained from tomato diseased roots in tomato protected areas. Take tomato diseased roots, wash the surface soil with distilled water, pick out the egg mass inside the root knot with a dissecting needle, put it in a petri dish filled with sterile water, and let it stand for incubation at 28°C for 24 hours; collect the hatched root-knot nematode second-instar larvae, Gently mix in the Erlenmeyer flask to obtain nematode suspension (about 100 / mL), and store at 4°C for later use.

[0034] 1.3 Experimental design

[0035] Using the collected tomato root-knot nematode as the test nematode, the anti-root-knot nematode activity of the 24-well plate was tested indoors ...

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Abstract

The invention provides Bacillus aerophilus ZDXC-01. The collection number of Bacillus aerophilus ZDXC-01 is CGMCC (China general microbiological culture collection center) No.14104. The invention further provides a 16S rRNA gene segment of the strain, a fermentation liquid, a fermentation method, an application of the strain in prevention of meloidogyne and the like.

Description

technical field [0001] The invention belongs to the technical field of microbes. Specifically, the invention relates to a new aerophilic bacillus isolated from seawater, which can prevent and control root-knot nematodes after fermentation. Background technique [0002] Bacillus aerophilus ( Bacillus aerophilus ) are mostly isolated from the ocean, and different strains have different properties. For example, Chinese patent application 201410168127 discloses a Bacillus aerophilus with a preservation number of CGMCC No. 8840, which is used to degrade petroleum hydrocarbons and improve ocean governance. Efficiency of oil pollution; as another example, Chinese patent application 201510314671 discloses a Bacillus aerophilus FE2, its preservation number is CGMCC No. 10662, and its fermentation supernatant can completely inhibit the production of aflatoxin, which is used for feed, grain Effective prevention and control of aflatoxin pollution in field crops, field crops, etc. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/68C12Q1/04A01N63/02A01P5/00C12R1/07
CPCA01N63/10C12N1/20C12Q1/689C12N1/205C12R2001/07
Inventor 雍道敬邱森森胡修俊范建龙
Owner CHINA KINGDOM AGRITECH QINGDAO
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