Preparation for prevention and treatment of tobacco and tomato meloidogyne spp. and application thereof

A technology for tomato root-knot nematodes and preparations, applied in the directions of nematicides, applications, biocides, etc., can solve the problems of environmental pollution of chemical nematicides, unsafe human and livestock, etc., and achieves easier popularization and application, promotion of plant growth, application of Simple and easy method

Active Publication Date: 2016-04-27
MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no nematode-resistant tomato varieties for production in China, and chemical control is mostly used to control the disease. However, chemical nematicides cause serious environmental pollution and are not safe for humans and animals during use. Many highly toxic chemical nematicides have been banned.

Method used

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  • Preparation for prevention and treatment of tobacco and tomato meloidogyne spp. and application thereof
  • Preparation for prevention and treatment of tobacco and tomato meloidogyne spp. and application thereof
  • Preparation for prevention and treatment of tobacco and tomato meloidogyne spp. and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 Acquisition, identification and preservation of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KC of the present invention

[0023] 1. Obtaining of Bacillus amyloliquefaciens YN2010-KC of the present invention

[0024] 1.1 Materials

[0025] The soil was collected from the soil around the corn rhizosphere of the Houshan Farm of Yunnan Agricultural University.

[0026] 1.2 Culture medium preparation

[0027] Melt LB solid medium and PSA medium (potato sucrose agar medium), pour them into sterilized petri dishes, cool down, and make LB and PSA plates.

[0028] 1.3 Isolation and culture

[0029] A strain isolated and purified was named YN2010-KC by using dilution plate separation method and plate streak separation.

[0030] 2. Identification of Bacillus amyloliquefaciens YN2010-KC

[0031] 2.1. Morphological identification

[0032] The bacterial strain YN2010-KC was cultured on LB medium, cultured at 37°C for 36 hours, and then observed a...

Embodiment 2

[0037] Embodiment 2 The preparation method of the preparation for preventing and treating tobacco tomato root-knot nematode of the present invention comprises the following steps:

[0038](1) Inoculate Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KCCGMCCNo.5722 stored at -80°C on liquid LB medium, and culture it at 28-37°C for 24-36 hours to obtain activated Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010- KCCGMCC No.5722, inoculate the activated Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YN2010-KCCGMCCNo.5722 into the seed medium according to 10% inoculation amount, and culture on a shaking table for 36 hours at a culture temperature of 35°C and a rotation speed of 120rpm / min. , to obtain a primary seed liquid, the seed medium is a liquid LB medium, pH7.0~7.2;

[0039] (2) Transfer the primary seed solution to the secondary seed medium in the seed tank at 35°C with a rotation speed of 100 rpm / min, an air flow of 1000 L / h, and a ta...

Embodiment 3

[0042] Embodiment 3 preparation of the present invention is to the control test of tomato root-knot nematode

[0043] 1. Pot experiment

[0044] 1) Test material: the test is tomato variety Yunza No. 8 (susceptible root-knot nematode tomato variety, commercially available), and the control agent is 1.8% abamectin aqueous emulsion (purchased from Shandong Zhongnong United Biotechnology Co., Ltd.) The inoculation nematode is root-knot nematode (Meloidogyneincognita).

[0045] The root-knot nematode (Meloidogyneincognita) was isolated from the soil according to the Baermann funnel method, and was identified morphologically based on the shape of the head and the pattern of the perineum. After being identified as root-knot nematode (Meloidogyneincognita), it was used for the experiment.

[0046] 2) Experimental design: Tomatoes were planted in pots, and the seedlings were transplanted when they had 4 true leaves. Take plastic pots of the same size and put the soil after drying a...

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Abstract

The invention discloses a preparation for prevention and treatment of tobacco and tomato meloidogyne spp. and an application thereof. The preparation for prevention and treatment of tobacco and tomato meloidogyne spp. has an active ingredient of a fermentation liquid of a strain of bacillus amyloliquefaciens YN2010-KC CGMCC No.5722. The preparation not only is a microbial fertilizer which can significantly promote the growth of plants, but also is a biological pesticide and has the average relative control efficiency on tobacco meloidogyne spp. disease of up to 78.32%; the preparation has the control efficiency on the tomato meloidogyne spp. disease of up to 74.50% or more, and has remarkable prevention and treatment effect. The preparation has low production cost, an application method is simple and easy to implement, and the preparation can be used for direct seed dressing and root irrigation, has no toxicity, no pollution and no residue, and is safe to human and livestock.

Description

technical field [0001] The invention relates to the prevention and control of tobacco and tomato root-knot nematodes, in particular to a strain of bacillus amyloliquefaciens and its application to the control of tobacco and tomato root-knot nematodes. Background technique [0002] Root-knot nematode (Meloidogynes pp.) disease is a serious disease that mainly occurs in tropical and temperate regions. Its host range is very wide, more than 5500 crop species, including monocots, dicots, herbaceous plants and woody plants. After the plant is invaded by the root-knot nematode, giant cells and root knots are produced in the root system, and the root-knot nematode absorbs nutrients from them to complete the life cycle. After the plants are harmed by root-knot nematodes, it is easy to cause yield reduction, plant wilting, and it is easy to be infected by other soil-borne diseases. [0003] Tobacco root-knot nematode (Meloidogynespp.) is one of the limiting factors of tobacco produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P5/00
CPCA01N63/10
Inventor 吴毅歆何月秋何鹏飞何鹏搏杨发祥
Owner MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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