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Quasi-trichoderma harzianum strain and application thereof

A technology of Trichoderma harzianum and strains, applied in the field of microorganisms, can solve problems such as unsafe, chemical control and pollution of the environment, and achieve the effects of high control effect, simple and practical process, and stable control effect.

Active Publication Date: 2015-12-09
MOON (GUANGZHOU) BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of Trichoderma pseudoharzianum strain and application thereof in order to solve the pollution environment and unsafe problems existing in chemical control in the prior art

Method used

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  • Quasi-trichoderma harzianum strain and application thereof
  • Quasi-trichoderma harzianum strain and application thereof
  • Quasi-trichoderma harzianum strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Isolation, purification and identification of Trichoderma:

[0028] Take soil samples evenly from the tomato planting fields in Shunyi, mix 10g of soil with 100ml of sterilized water to make a suspension, and then carry out gradient dilution in turn, take 100μl of the soil mixture diluted 10000 times and spread it on the PDA medium plate. That is, 200g of potatoes, 20g of glucose, 17g of agar, and distilled water to 1000ml. The culture medium was sterilized at 121°C and plated in petri dishes. PDA medium contains 75 μg / ml ampicillin, which inhibits bacterial growth. The culture medium was cultured at 25°C. After the tissue pieces grew colonies, single spores were isolated, and single colonies were picked and purified on PDA medium for classification and identification. The strains were morphologically determined according to the Fungal Identification Manual.

[0029] Pick the mycelium of the colony, put it into an EP tube containing 300 μl of fungal lysate, and lyse ...

Embodiment 2

[0034] Preparation and application of Trichoderma pseudoharzianum seed coating agent:

[0035] The Thar2 strain was inoculated into 250ml Erlenmeyer flasks, each containing 125ml of PDB liquid medium, and cultured at 25° C. with shaking (160 rpm) for 8 days to obtain a large number of mycelia and conidia. The liquid fermentation medium is a potato dextrose liquid (PDB) medium, that is, 200 g of potatoes, 20 g of glucose, and distilled water to a volume of 1000 ml.

[0036] The spore mycelium mixed solution obtained by liquid shaking culture (fermentation), the spore concentration is 10 7 pcs / ml), add the following ingredients: the ratio is as follows: 1L of spore liquid, 500g of diatomaceous earth, 200g of corn flour, and 50g of wheat bran. The solid fermented product is placed at room temperature and naturally dried for 3 days (the water content of the fermented product is controlled between 15-25%), crushed and passed through a 60-mesh sieve to obtain spore powder. The spo...

Embodiment 3

[0038] Preparation and application of Trichoderma pseudoharzianum seed coating agent:

[0039] The Thar2 strain was inoculated into 250ml Erlenmeyer flasks, each bottle containing 125ml of PDB liquid medium, cultured at 28° C. with shaking (160 rpm) for 6 days to obtain a large number of mycelia and conidia. The liquid fermentation medium is a potato dextrose liquid (PDB) medium, that is, 200 g of potatoes, 20 g of glucose, and distilled water to a volume of 1000 ml.

[0040]The spore mycelium mixed solution obtained by liquid shaking culture (fermentation), the spore concentration is 10 10 pcs / ml), add the following ingredients: the ratio is as follows: 2L of spore liquid, 700g of diatomaceous earth, 400g of corn flour, and 150g of wheat bran. The solid fermented product is placed at room temperature to dry naturally for 5 days (the water content of the fermented product is controlled between 15-25%), crushed, and passed through a 60-mesh sieve to obtain spore powder. The s...

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Abstract

The invention discloses a quasi-trichoderma harzianum strain and an application thereof. The stain is quasi-trichoderma harzianum strain Thar2 which is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO.10924. For the application of a biocontrol seed coating agent prepared from the quasi-trichoderma harzianum strain Thar2 in prevention and treatment of Meloidogyne, the quasi-trichoderma harzianum Thar2 strain is a fungus obtained by being separated from soil, is harmless to crops, has prevention and treatment functions for the Meloidogyne, has safety in the application and is suitable for biological control of the Meloidogyne. The death rate of quasi-trichoderma harzianum Thar2 strain fermentation broth on second instar larvae of the Meloidogyne reaches over 98.8 percent, the prevention and treatment efficiency in a greenhouse reaches 83.5 percent, the prevention and treatment efficiency is high and stable, and the quasi-trichoderma harzianum Thar2 strain fermentation broth is suitable for agricultural production requirements.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Trichoderma pseudoharzianum strain and application thereof. Background technique [0002] Root-knot nematode (Meloidogyne) is a highly specialized omnivorous plant pathogenic nematode. Nematodes known to harm vegetables mainly include root-knot nematode peanut, root-knot nematode northern, root-knot nematode southern, root-knot nematode java, and root-knot nematode beet. Nematodes have a wide range of hosts, often harming more than 30 kinds of vegetables such as melons, solanaceous fruits, beans, radishes, carrots, lettuce, and cabbage, and can also spread some fungal and bacterial diseases. Root-knot nematode is an important crop pathogen, causing losses in the order of billions of dollars each year. Root-knot nematodes mainly infect the roots of crops. After infection, the roots swell to form huge root knots, which can be connected to form beads. Because the roots ar...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/885
Inventor 茆振川张晓平马玉琴凌键陈国华杨宇红谢丙炎
Owner MOON (GUANGZHOU) BIOTECH CO LTD
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