Meloidogyne culture medium and culture method

A root-knot nematode and culture medium technology, applied in animal husbandry, additional food elements, animal feed, etc., can solve the problems of long cultivation time, dependence on root-knot nematode cultivation, cumbersome cultivation methods, etc., to shorten the cycle and increase production Effect

Active Publication Date: 2021-12-24
广东博沃特生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems that the cultivation of root-knot nematode depends on the host, but the existing cultivation method is cumbersome to operate, the cultivation time is long, and the number of nematodes is difficult to meet the research needs, etc., the present invention discloses a culture medium and cultivation method for plant root-knot nematode

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  • Meloidogyne culture medium and culture method
  • Meloidogyne culture medium and culture method
  • Meloidogyne culture medium and culture method

Examples

Experimental program
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preparation example Construction

[0020] 3. Preparation of root-knot nematode

[0021] Southern root-knot nematode pathogen collection: looking for serious morbidity in tomato plants cultivated land farmers, in its entirety out (try to keep the roots intact), remove large clods brought roots, retaining a little soil attached to the roots, the root system and placed in sterile soil in a ziplock bag, ice box immediately transported back to the lab.

[0022] Tomato Root Isolation: Root taken back rinse with tap water, rinsed with distilled water 1-2 times. The washed roots cut into segments of 1-2cm flask, was added 0.5% sodium hypochlorite, the oscillation 2 ~ 3min. Mixture was repeatedly washed with sterile water, and 500 mesh to collect eggs and larvae on the 800 mesh sieve with 3 layers were separated (the top 60 mesh, 500 mesh middle and the lower 800 mesh).

[0023] Egg hatch: mesh placed in a clean Kimwipe paper layer 2, then placed on a glass petri dish, pour the collected eggs sieve paper, distilled water wa...

Embodiment 1

[0024] Example 1: Powder Compound of different levels of the amino acid to affect the results of culture plants Meloidogyne

[0025] Water agar: The agar 10g, distilled water was added to 0.4g calcium chloride dihydrate in 1000mL, after dissolution with pH = pH 0.2M phosphate buffer solution was adjusted to 7.6 7.2,121 ℃ 21min sterilized, divided attached to the dish aside.

[0026] Clearly the optimal addition amount of the medium powder compound amino acid, an amino acid compound powder added in an amount set on the basis of the water-agar medium: 0,0.5g, 1g, 1.5g, 2g, 2.5g, 3g / L, study its effect on plant cultivation of root-knot nematode.

[0027] DETAILED respectively according 0,0.5g, 1g, 1.5g, 2g, 2.5g, 3g / L of an amount of water added to the agar medium, after dissolution with pH = 0.2M phosphate buffer solution pH 7.6 was adjusted to 7.2,121 ℃ 21min sterilized, dispensed into petri dishes, spare.

[0028] Sterile operating table, taking 2mL100 bar / mL of suspension w...

Embodiment 2

[0032] Effects of Example 2 different addition amounts of fish meal in vitro culture of plant embodiment Meloidogyne

[0033] Water agar: The agar 10g, distilled water was added to 0.4g calcium chloride dihydrate in 1000mL, after dissolution with pH = pH 0.2M phosphate buffer solution was adjusted to 7.6 7.2,121 ℃ 21min sterilized, divided attached to the dish aside.

[0034] To clarify optimum addition amount of the medium fish meal, fish meal is added in an amount set based on a water agar medium: 0,1g, 2g, 2.5g, 3g / L, study the in vitro culture of plant root-knot nematodes .

[0035] DETAILED respectively according 0,1g, 2g, 2.5g, 3g / L of an amount of water added to the agar medium with pH = 0.2M phosphate buffer solution pH was adjusted to 7.6 after sterilization dissolved to 7.2,121 ℃ 21min, dispensed into petri dishes, spare.

[0036] Sterile operating table, taking 2mL100 bar / mL of suspension was inoculated to the root-knot nematode larvae plates containing the corres...

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Abstract

The invention discloses a meloidogyne culture medium. The meloidogyne culture medium comprises compound amino acid powder, fish meal, calcium chloride dihydrate, agar powder and water. By adding the compound amino acid powder and the fish meal into the water agar culture medium, the yield of the meloidogyne can be greatly increased, and the period is shortened, so that the invention provides a simple, convenient and rapid culture method capable of obtaining a large number of plant meloidogyne, and the problems of dependence on hosts, tedious operation, long culture time and small meloidogyne number in the existing meloidogyne culture are solved.

Description

Technical field: [0001] The present invention belongs to the plant propagation and preservation BACKGROUND incognita, Meloidogyne plant culture media and more particularly to a method of culturing. Background technique: [0002] Root-knot nematode (Meloidogyne spp.) Is a plant pathogenic nematode in most species, the most widely distributed, against the most serious category nematodes, often causing serious losses agricultural and forestry economy. Miao Jinling, Zhao Xiaoyan and other studies have shown that root-knot nematodes can cause crop losses of 13% to 25%, serious plots up to 70% or more, even crops. According to statistics, the worldwide annual revenue loss caused by root-knot nematode rate has reached 10%, resulting in direct economic losses of more than 125 billion US dollars. With the rapid development of my country's new rural agricultural facility, root-knot nematodes harm there is an increasing trend. [0003] In recent years, control of plant root-knot nematode of...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K10/22A23K20/142A23K20/163A23K20/24A23K50/90
CPCA01K67/033A23K50/90A23K20/142A23K10/22A23K20/24A23K20/163
Inventor 李成江谢小林周莲陈猛冯曾威刘玉敏陈美标
Owner 广东博沃特生物技术有限公司
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