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Proteus mirabilis strain and method for producing S-equol through daidzein conversion by using the same

A technology of Proteus mirabilis and daidzein, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of difficulty in converting and preparing S-equol, etc.

Inactive Publication Date: 2013-02-13
HUAQIAO UNIVERSITY
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Problems solved by technology

The invention solves the problem that the transformation and preparation of S-equol existing in the prior art is difficult to realize, and provides a single-function microbial strain capable of directly converting the latent chiral compound daidzein into the chiral compound S-equol and its preparation method

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  • Proteus mirabilis strain and method for producing S-equol through daidzein conversion by using the same
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Embodiment Construction

[0047] The present invention will be further described below through the accompanying drawings and specific implementation, but it is not meant to limit the protection scope of the present invention.

[0048] A Proteus mirabilis strain LH-52 of this embodiment, the LH-52 strain is preserved in the China Center for Type Culture Collection, the preservation address is China. Wuhan. Wuhan University, the preservation number is CCTCC M 2011390, and the preservation date is 2011 November 13th. The preparation method of described Proteus mirabilis bacterial strain LH-52 is as follows:

[0049] 1. Collection of samples

[0050] Take the intestinal content within 5 cm of the rat intestinal cecum, suspend it in normal saline, filter to remove the solids, and then take 200 microliters of the filtrate and inoculate it in a freshly sterilized liquid medium for anaerobic activation culture at 37°C 24 hours. The fresh liquid culture medium formula described therein is: peptone 27.5 grams...

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Abstract

The present invention discloses a Proteus mirabilis strain and a method for producing S-equol through daidzein conversion by using the Proteus mirabilis strain, wherein the strain is preserved in the China Center for Type Culture Collection on November 13, 2011, and a preservation number is CCTCC M 2011390. In the prior art, the existing technology is difficult to prepare the S-equol through conversion. With the present invention, the problem in the prior art is solved, and a single function microorganism strain for directly converting a prochiral compound daidzein into a chiral compound S-equol and a preparation method thereof are provided; and compared with the strictly anaerobic equol conversion strains and the mixing bacteria conversion in the previous reports, the Proteus mirabilis LH-52 strain of the present invention is a facultative anaerobe, and is more suitable for industrial production.

Description

technical field [0001] The present invention relates to a Proteus mirabilis (Proteus mirabilis) strain (LH-52) and the strain can directly convert daidzein (daidzein) rich in soybeans into highly active phytoestrogen S-equol (S-equol) ) method, which belongs to the field of biochemical industry. Background technique [0002] Soy isoflavones have a similar structure to the estrogen estradiol and are widely known as "phytoestrogens". A large number of studies have found that soybean isoflavones with daidzein and genistein as the main components have a very wide range of biological activities. In addition to estrogen-like effects, they also have functions such as anti-oxidation and cell cycle regulation. Compared with daidzein and genistein, equol has higher estrogenic activity and antioxidant activity. Pathological studies have shown that equol has a significant effect on the prevention of breast cancer, prostate cancer, osteoporosis and menopausal syndrome. In particular, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/06C12R1/37
Inventor 肖美添
Owner HUAQIAO UNIVERSITY
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