The invention discloses single-dose pymetrozine. The single-dose pymetrozine comprises 10-80wt% of a pymetrozine solution, and can be processed to prepare a water dispersible granule of pymetrozine, a pymetrozine suspension and a wettable pulvis of pymetrozine. A pesticide composition containing pymetrozine comprises pymetrozine and thiacloprid, and a mass ratio of pymetrozine to thiacloprid is 80:1-1:80. Pymetrozine is prepared by extracting from a natural plant source raw material and processing, has the advantages of no pollution, no residual, advanced technology, good control effect and no influences on the quality of agricultural products, can be widely used in crops comprising grain crops, rice, corn, cotton, vegetables, fruits and the like, has active control effects on sucking pests of Heteroptera, Thysanoptera, Homoptera, Diptera and the like and harmful to the growth of the crops, such as plant hoppers, thrips, cicadas, leafhoppers, whiteflies, psyllids, aphids, scale insects, wheat midge, rice gall midges, mites, and is a new environmentally-friendly pesticide at present in China.