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Laboratory culture system and method for blood-sucking midges

A breeding method and breeding system technology, applied in animal husbandry and other fields, can solve problems such as difficult discovery, limited research, and increased difficulty in specimen collection

Inactive Publication Date: 2021-03-26
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Outbreaks of the above-mentioned diseases can cause huge economic losses
[0003] At present, the research on adult blood-sucking midges at home and abroad is relatively in-depth, but the juvenile research on midges is an extremely weak link. Compared with adults, juvenile insects are difficult to collect and find, which greatly increases the difficulty of specimen collection. , which limits the research on morphology, ecology and other aspects of juvenile

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  • Laboratory culture system and method for blood-sucking midges
  • Laboratory culture system and method for blood-sucking midges
  • Laboratory culture system and method for blood-sucking midges

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Embodiment 1

[0022] A laboratory breeding system for blood-sucking midges, which consists of four parts: an adult feeding tube 1, an egg feeding dish 2, a larva feeding dish 3, and a pupa feeding dish 4. The adult feeding tube 1 is a glass test tube 11 with a specification of 10×150 mm. The outer diameter of the glass test tube 11 is 15 mm, the length is 150 mm, and the color is transparent. One female worm per tube is convenient for observing the oviposition situation of adults. Circular filter paper A13 with the same diameter at the mouth of the tube is convenient for adults to lay eggs here. The top opening of the glass test tube 11 is provided with absorbent cotton B14 to prevent blood-sucking midges from flying out, and the bottom of the absorbent cotton can be dipped in nutrient solution, etc. for feeding; Described egg rearing dish 2 is the petri dish A21 of specification 60mm, and the loam cake A22 diameter of petri dish A21 is 64mm, and bottom diameter is 60mm, dish height is 15mm,...

Embodiment 2

[0024] A laboratory breeding method for blood-sucking midges, comprising the steps of:

[0025] 1) Cultivation of blood-sucking midges after collection: Before collection, drip distilled water into the bottom of the culture tube until the absorbent cotton A12 and filter paper A13 are wet, but not too much, to avoid excessive water that will cause adults to stick to the filter paper A13 and cannot fly; then according to When blood-sucking midges bite humans and animals in the local area, set a collection point. When collecting, expose one side of the lower leg to attract blood-sucking midges. One tube is one adult. When the blood-sucking midges stop and suck blood, remove the absorbent cotton B14 at the mouth of the glass test tube 11 , the mouth of the glass test tube 11 is buckled on the skin of the blood-sucking midge. After the blood-sucking midge is full of blood, tap the glass test tube 11 with your fingers to make the females leave the skin, and then block the mouth of th...

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Abstract

The invention discloses a laboratory culture system and method for blood-sucking midges. The system comprises an adult feeding pipe, an egg feeding vessel, a larva feeding vessel, a pupa feeding vessel, a constant-temperature box, filter paper, degreasing cotton, tweezers, a wet writing brush and a plastic cover. The method comprises the following steps of (1) culturing after collecting the satiety blood-sucking midges; (2) culturing in a period of hatching eggs into larvae; (3) culturing in a larva period; and (4) culturing in a pupa period. The method is simple to operate, fills the technical blank of laboratory culture of the blood-sucking midges, and provides great support for scholars to carry out laboratory research on the blood-sucking midges.

Description

technical field [0001] The invention relates to a laboratory breeding system and method for blood-sucking midges. Background technique [0002] Blood-sucking midges, belonging to Diptera, Longocera, and Ceridae, are a kind of midges that can transmit insect-borne pathogens by sucking the blood of ruminants, and are a kind of complete metamorphosis insects. Its growth and development can be divided into four stages: egg, larva, pupa, and adult. A total of 1 671 species are known in the world, and 443 species of blood-sucking midges are known in China. Blood-sucking midges are small in size, various in variety, and widely distributed. They can suck the blood of humans and animals, carry Japanese encephalitis virus, and spread bluetongue disease in cattle and sheep, chicken protozoa, and African horse sickness. They are important vector insects . The outbreak of the above-mentioned diseases will bring huge economic losses. [0003] At present, the research on adult blood-su...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 侯晓晖甯媛卢雪
Owner ZUNYI MEDICAL UNIVERSITY
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