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Slow release biological agent for killing larvae of midge, preparation method and application method

A technology for biological preparations and chironomid larvae is applied in the field of preparation of slow-release biological preparations for killing chironomid larvae, and can solve the problems of waste and low persistence of preparations.

Inactive Publication Date: 2006-12-06
SHENZHEN KITEWAY AUTOMATION ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when in use, the various preparations have low persistence and need to be frequently put into water bodies to ensure the concentration of active ingredients for killing chironomus larvae; meanwhile, because B.t.i degrades rapidly in natural water bodies, it causes a lot of waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A slow-release biological preparation for killing chironomus larvae, including an active ingredient, a carrier and a cross-linking agent, the active ingredient includes the thallus of Bacillus thuringiensis subsp. Including sodium alginate, the cross-linking agent includes calcium chloride; the active ingredient thallus is a powder prepared by fermentation of Bacillus thuringiensis subspecies Israel (abbreviated as B.t.i powder). The 24-hour semi-lethal concentration is not higher than 0.2mg / L, and the mass ratio of powder to sodium alginate is 1:1.

[0019] The mass ratio of the above powder to sodium alginate can also be 1:1.5 or 1:2.

Embodiment 2

[0021] A preparation method of a slow-release biological preparation for killing chironomus larvae, characterized in that it comprises the steps of:

[0022] S101: Preparation of the active ingredient, fermenting Bacillus thuringiensis subsp. Israel, plate-and-frame filter press, and spray-drying to obtain a powder. The semi-lethal concentration of the obtained powder against Chironomus III instar larvae is lower than 0.2 mg / L;

[0023] S102: The carrier sodium alginate is prepared into a solution with a concentration of 2%, and it is used after high-temperature and high-pressure sterilization. The powder obtained in step S101 is prepared with sterile water into a solution with a concentration of 1%, and then added according to a volume ratio of 1:1 into the solution of sodium alginate, and mechanically mix evenly; (in this step, the carrier sodium alginate can also be formulated into a solution with a concentration of 3% or 4%, and the powder obtained in step S101 is formulate...

Embodiment 3

[0027] The application method of the slow-release biological preparation for killing chironomus larvae, in the application environment of water source waters, the usage amount of the slow-release biological preparation described in Example 1 is to make the active ingredient of B.t.i powder reach 700mg / m 2 .

[0028] It is also possible to make the active ingredient of the above B.t.i powder reach 500, 600, 800 or 1000mg / m 2 .

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Abstract

A slow-releasing biologic product for killing the midge larvae in water source, water region and waterworks is prepared from the Israeli subset of Bacillus thuringiensis (B.t.i), sodium alginate as carrier and cross-linking agent through fermenting and reproduction to obtain B.t.i powder, dissolving others, mixing and cross-linking reaction.

Description

technical field [0001] The invention relates to a water pollution prevention and control technology, in particular to a preparation method and application method of a slow-release biological preparation for killing chironomus larvae, and is mainly applicable to surface water, landscape water, drinking water sources, drinking water, etc. Water treatment process water and other waters. Background technique [0002] In polluted surface water, landscape water, water source water, and water supply systems that use polluted water bodies as water sources, chironomids are easy to breed, and chironomus larvae are red in color, commonly known as "red worms". Although chironomids are non-biting mosquitoes, they are harmful in many ways. Chironomus larvae that breed during the water supply process will cause strong sensory stimulation to the senses of users. Although it has not been widely proven, research results on pathogenic bacteria carried by chironomus have been reported from tim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00C02F1/50
Inventor 张金松刘丽君陈益清尤作亮周令丁书江
Owner SHENZHEN KITEWAY AUTOMATION ENG
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