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Low molecular Brazil mushroom polysaccharide and its prepn process and application in antagonizing tumor metastasis

A technology of Agaricus blazei polysaccharide and low molecular weight, which is applied in the application of the Agaricus blazei polysaccharide in anti-tumor metastasis drugs, and the preparation field of the Agaricus blazei polysaccharide can solve the problems of high toxicity, poor tumor control effect, and inconspicuous effect , to achieve the effect of stable performance and good development prospects

Inactive Publication Date: 2007-11-07
QIQIHAR MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these therapies are not effective in controlling the metastatic behavior of tumors
About 30% of newly diagnosed patients with solid tumors have clinically visible metastases, and the currently discovered anti-metastatic agents such as retinamide, paclitaxel, arabinogalactose, and heparin are all obtained by chemical synthesis methods, with relatively high toxicity and low efficacy. not obvious

Method used

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  • Low molecular Brazil mushroom polysaccharide and its prepn process and application in antagonizing tumor metastasis
  • Low molecular Brazil mushroom polysaccharide and its prepn process and application in antagonizing tumor metastasis
  • Low molecular Brazil mushroom polysaccharide and its prepn process and application in antagonizing tumor metastasis

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preparation example 1

[0023] Preparation example 1: preparation of medicine of the present invention

[0024] Take Agaricus blazei fruiting body 5kg and crush it; take Agaricus blazei fruiting body powder through a 40-mesh sieve, add 50kg of distilled water, and extract at 100°C for 3 hours; after the extract is centrifuged by a high-speed centrifuge, add 50kg of distilled water to the residue and heat to 45 Add the following compound enzymes at ℃ according to the dry weight of raw materials: papain 1.0%, cellulase 0.5%, pectinase 1.0%, enzymolysis for 2 hours, then warm up to 100 ℃, keep at 100 ℃ for 3 hours, and remove by high-speed centrifugation scum. The two extracts were combined and vacuum concentrated to 1 / 3 of the original volume, then 4 times the amount of 95% ethanol was added, precipitated for 24 hours, and the precipitate was separated from the ethanol solution to obtain Agaricus blazei crude polysaccharide; % neutral protease, adjust pH value to 5.0, enzymolysis at 40°C for 60min, he...

preparation example 2

[0025] Preparation example 2: preparation of medicine of the present invention

[0026] Grind 5 kg of Agaricus blazei fruiting body; take Agaricus blazei fruiting body powder through a 40-mesh sieve, add 75 kg of distilled water, and extract at 100°C for 2 hours; after the extract is centrifuged in a high-speed centrifuge, add 75 kg of distilled water to the residue and heat to 50 Add the following compound enzymes with the amount of enzyme calculated according to the dry weight of raw materials: papain 1.5%, cellulase 1.0%, pectinase 1.0%, enzymolysis for 1.5h, then heat up to 100°C, keep at 100°C for 2h, and centrifuge at a high speed Go to the slag. After the two extracts were combined and concentrated to about 1 / 4 of the original volume, 5 times the amount of 90% ethanol was added, precipitated for 18 hours, and the precipitate was separated from the ethanol solution to obtain the Agaricus blazei crude polysaccharide; the Agaricus blazei crude polysaccharide was used 1.5%...

preparation example 3

[0027] Preparation example 3: preparation of medicine of the present invention

[0028] Crush 4 kg of Agaricus blazei fruiting bodies; take Agaricus blazei powder through a 40-mesh sieve, add 80 kg of distilled water, and extract at 100°C for 2 hours; after the extract is centrifuged in a high-speed centrifuge, add 80 kg of distilled water to the residue, heat to 45°C and add Enzyme dosage is the following compound enzymes calculated according to the dry weight of raw materials: 1.0% papain, 1.0% cellulase, 1.0% pectinase, heat up to 100°C after 2 hours of enzymolysis, keep at 100°C for 3 hours, and remove residue by high-speed centrifugation. Combine the two extracts and concentrate in vacuum to about 1 / 4 of the original volume, then add 3 times the amount of 95% ethanol, precipitate for 24 hours, and separate the precipitate from the ethanol solution to obtain Agaricus blazei crude polysaccharide; Agaricus blazei crude polysaccharide Use 1.0% neutral protease to adjust the p...

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Abstract

The present invention discloses one kind of low molecular Brazil mushroom polysaccharide and its preparation process and application in antagonizing tumor metastasis. The low molecular Brazil mushroom polysaccharide extracted from the sporophore of edible fungus Brazil mushroom has homogeneous glucosan in molecular weight of 48,000 as the main component. It is prepared with sporophore of Brazil mushroom, and through crushing, leaching in distilled water, precipitating in alcohol to obtain coarse Brazil mushroom polysaccharide product, deproteinizing, chromatographic separation in DEAE650M cellulose column, gradient eluting with NaCl solution, chromatographic separation of eluted liquid in Toyopearl HW-65F column, eluting with NaCl solution, chromatographic separation of eluted matter in Toyopearl HW-50S column, eluting with NaCl solution, and drying.

Description

Technical field: [0001] The invention relates to a low-molecular-weight Agaricus blazei polysaccharide, a preparation method of the Agaricus blazei polysaccharide, and an application of the Agaricus blazei polysaccharide in anti-tumor metastasis drugs. Background technique [0002] Agaricus blazei, the scientific name is mushroom, because it is naturally distributed in Sao Paulo, Brazil, South America, also known as Brazil mushroom, it is a precious medicinal and edible fungus. In terms of fungal classification, it belongs to Basidiomycotina, Phytomycota, Agaricaceae, Mushroomaceae, Mushroom genus, and the Latin scientific name is Agaricus blazei Murill. In the 1960s, Agaricus was successfully introduced and cultivated by Japan, and then spread to Vietnam, Thailand, Indonesia, Taiwan and other East Asian regions, and was introduced to my country in the early 1990s. Now it is widely cultivated in Fujian and Zhejiang. At the same time, the liquid culture technology related to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02A61K31/716A61K36/06A61P35/00
Inventor 刘吉成牛英才
Owner QIQIHAR MEDICAL UNIVERSITY
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