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Cultivation method of fungus hexagonia apiaria fruit body

The technology of a hairy cellulite and cultivation method is applied in the field of cultivation of the hairy cellulite fruit body, which can solve problems such as not being easy to obtain, unable to satisfy research and development, and unable to realize large-scale cultivation of the hairy cellulite fruiting body, so as to achieve quick results and high production short cycle effect

Inactive Publication Date: 2009-05-06
GUANGDONG INST OF MICROORGANISM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Hair cell fungus is distributed in tropical and subtropical regions. It is a kind of polypore medicinal fungus that likes temperature and humidity. Due to the influence of environmental factors, wild hair cell fungus is very rare in nature and is not easy to obtain, which cannot meet the needs of research and development. need
The artificial domestication and cultivation of Trichocellus have not been reported at home and abroad, and the cultivation of Trichocellus fruiting bodies cannot be achieved in large quantities.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The present invention obtains the hairy cellulite by collecting the fruiting body of the wild hairy cellulite through tissue separation and purification, and liquid cultures the obtained hairy cellulite to obtain the liquid seed mycelium, and uses the fungus bag to cultivate the mycelium with the cultivation material, and the mycelium develops to maturity Open the bottle cap of the bacteria bag, put it into an artificial climate box for cultivation, and pick it after growing mature fruiting bodies. The specific operation steps are as follows:

[0048] A. Isolation and purification of hair cellulite

[0049] (1) Preparation of comprehensive PDA slant medium

[0050] Potatoes 200g

[0051] Glucose 20g

[0052] K H 2 PO 4 1g

[0053] MgSO 4 0.5g

[0054] Vitamin B 1 10mg

[0055] Agar 20g

[0056] Distilled water 1000mL

[0057] Weigh the above formula by weight, boil it, filter it with gauz...

Embodiment 2

[0086] Step D: The cultivation conditions for the fruiting bodies of Hairy cellulosum are as follows: place the fungus bags in an artificial climate box with a temperature of 24° C., a relative air humidity of 80%, a light intensity of 1000 LUX, and 10 hours of light per day. The hyphae of the mushroom bag grow mature fruiting bodies after 80 days of growth and cultivation.

[0087] Other steps are identical with embodiment 1.

Embodiment 3

[0089] Step D: The cultivation conditions for the fruiting bodies of Hairy cellulosum are as follows: place the fungus bags in an artificial climate chamber at 30° C., relative air humidity of 85%, light intensity of 1200 LUX, and 12 hours of light per day. The hyphae of the mushroom bag grow mature fruiting bodies after 60 days of growth and cultivation.

[0090] Other steps are identical with embodiment 1.

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Abstract

The invention discloses a method for planting fungi hexagona apiaria (CCTCC NO.M208121) sporocarp for a rare drug. The method comprises the following steps: the cultivation of a separated and purified hexagona apiaria seed, the cultivation of hypha in a fungus sack, the cultivation of the hexagona apiaria sporocarp and the harvest of the hexagona apiaria sporocarp. The planting of the hexagona apiaria sporocarp through a sack material needs about 2 to 3 months from the production of a strain, inoculation, fungus cultivation, fruiting cultivation to the harvest of the sporocarp; the method has short production period and rapid effect, realizes large-scale cultivation of the hexagona apiaria sporocarp, fundamentally solves the problem of insufficient wild resources, provides abundant drug resources for further research of the active components, the pharmacological action, a pharmaceutical mechanism and a toxicity test of the hexagona apiaria sporocarp, and lays a foundation for better developing and utilizing the hexagona apiaria.

Description

technical field [0001] The present invention relates to the cultivation method of medicinal fungus, more specifically, relates to the cultivation method of the fruit body of Hexagonia apiaria. Background technique [0002] Medicinal fungi are fungi used to prevent, inhibit, and treat diseases. In clinical applications, they either directly use mycelia, sclerotia, or fruiting bodies, or use effective substances isolated from the fungi. At present, there are 400 kinds of medicinal fungi with traditional and experimental medicinal effects, but only a few dozen kinds are widely used in clinical practice. Therefore, vigorously carrying out scientific research on medicinal fungi is of great significance for the development of fungal health food and new drugs. [0003] Hair cell fungus [Hexagonia apiaria (Pers.) Fr.], has another name called longan comb, belongs to non-fold fungus (Aphyllophorales), polyporaceae (Polyporaceae), honeycomb fungus (Hexagonia) (hair cell of the presen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 李浩华章卫民潘清灵王磊陶美华
Owner GUANGDONG INST OF MICROORGANISM
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