36 results about "Curvularia lunata" patented technology

The invention provides actinomycetes which is streptomyces samsunensis XJC-2-6, and the preservation number is CCTCC NO:M 2017620. The streptomyces samsunensis XJC-2-6 provided by the invention has anantagonism on fusarium oxysporum f.sp cubense No. 4, curvularia fallax, curvularia lunata, btoryosphaeria dothidea, colletotrichum acutatum, colletotrichum gloeosporioides, colletotrichum fragariae,botrytis cinerea, fusarium oxysporum (schl.) f.sp cucumerinum owen, colletotrichum acutatum, pyricularia oryae cav, fusahum graminearum sehw, botryosphaeria dothidea and the like, has wide developmentspace on prevention and treatment of Panama disease of banana, microscler streak disease of banana, large grey speck disease of banana, tree canker of banana, anthracnose of pepper, anthracnose caused by colletotrichum gloeosporioides, anthracnose of strawberry, disease caused by botrytis cinerea, fusarium wilt of cucumber, anthracnose of mango, rice blast, wheat scab, ring rot of apple and the like, and has very good development and application prospects.

The invention discloses a steroid 11 beta-hydroxylase in curvularia lunata and a coding gene and an application thereof. The steroid 11 beta-hydroxylase is a protein (CL3-CYP021 protein) shown in SEQID No.1. In addition, a protein set composed of the steroid 11 beta-hydroxylase and the protein (CL3-CPR protein) shown in SEQ ID No.2 is further provided. Catalyzed synthesis of hydrocortisone (HC) and 14 alpha-hydroxylated cortisol is conducted by expressing CL3-CYP021 protein (or CL3-CYP021 and CL3-CPR protein) in a heterologous microorganism. The original filamentous fungus is replaced yeast for biocatalytic fermentation to produce HC. The advantages of shortening the fermentation time, simplifying the fermentation condition and separating and extracting steps and reducing the production cost can be achieved. The steroid 11 beta-hydroxylase has significant importance on replacement of the traditional hydrocortisone biocatalytic fermentation production mode.

The invention discloses a biocontrol strain and agent capable of preventing and controlling plasmopara viticola and phytophthora capsici and an application of the biocontrol strain and agent. The biocontrol strain is characterized by being a streptomyces atratus py-1 strain in surface soil of a grapery and is collected with the number of China General Microbiological Culture Collection Center (CGMCC) No:7826 in CGMCC; and a secondary metabolite of streptomyces atratus py-1 is used as an active component of the biocontrol strain. The biocontrol agent is prepared through the steps of inoculating the streptomyces atratus py-1 into 200ml of psa culture solution in a 500ml triangular flask to culture for 5 days; centrifuging a fermentation solution for 30 minutes; filtering a supernatant solution by using a filter membrane to obtain a stock solution; diluting the stock solution, then, spraying the diluted stock solution on grapes, and irrigating the diluted stock solution into the roots of hot peppers. The biocontrol agent has a certain effect on inhibiting egg plant stem blight, egg plant root rot, tomato spotted wilt virus, tomato late blight, cucumber anthracnose, maize ear rot, sorghum curvularia lunata and wheat root rot.

The invention relates to a method for genetic transformation of germinating conidiophore of Curvularia lunata Boed. The method comprises the following steps: culturing the germinating conidiophore of the Curvularia lunata Boed as a receptor by a slide suspension method; co-culturing agrobacterium containing an epiphyte conversion vector system and the germinating conidiophore; and screening transformants with resistibility on an antibiotic culture medium. The system uses the Curvularia lunata Boed as initial bacteria, spore is scratched by 0.9 percent of physiological saline after the Curvularia lunata Boed is cultured on the PDA culture medium for 5 to 7 days, and is used for infection of the agrobacterium after the spore is germinated by the slide suspension culture method. The infected germinating conidiophore obtains resistant transformant by co-culture, bacteriostasis culture and anti-selective agent screening culture of continuous two generations, so that a Curvularia lunata Boed mutant base is constructed to provide rich screening strain sources for weak pathogenicity and pathogenic defective Curvularia strain, and meanwhile can be used for cloning related gene of Curvularia lunata Boed pathogenicity so as to lay a foundation for further investigating the pathogenesis mechanism of the Curvularia lunata Boed strain.

The invention relates to a method for constructing a mutant strain of maize curvularia leaf spot fungus. The curvularia lunata strain is picked up to be subject to the intermediate culture to prepare the protoplast suspending liquid; colon bacillus strain in cold-preservation containing plasmids is picked up to be subject to the inoculation culture to be extracted plasmid DNA and prepare the enzyme-plasmid mixing liquid; the protoplast suspending liquid and the enzyme-plasmid mixing liquid are evenly mixed and subject to the culture and the filtration to obtain mutant, and the mutant is cultured on PDAs with hygromycin and without hygromycin, and is filtered to obtain the stable mutant strain of maize curvularia leaf spot fungus. The method adopts the restriction Enzyme-mediated gene integration technology to mix the plasmid DNA and the prepared trichoderma protoplast and makes the exogenous linear plasmid fragments inserted in the genome to induce the insertion mutation under the action of the restriction Enzyme-mediated. The mutant strain obtained by the method provides abundant filtrating bacteria sources for the filtration of the weak pathogenic curvularia lunata strain, and can be used to the cloning of genes related to the maize curvularia lunata pathogenicity and the research on the pathogenesis mechanism of the maize curvularia lunata strain.

The invention discloses a zopfiella sp. multifunctional biocontrol characteristic strain. A preservation number of zopfiella sp. is CGMCC No. 60123. The zopfiella sp. BJF10 has strong inhibition effect for fusarium moniliforme, fusarium oxysporum, curvularia lunata, and pythium, through determination of cellulose degrading capability, CMC enzyme activity can achieve as high as 180.32 U / mL, and filter paper enzyme activity can achieve as high as 81.46 U / mL. Through determination of antioxidation capability, and the strain fermentation product has an average removing rate on DPPH can achieve 67.7%.

The invention relates to a method for preparing streptomyces fermentation liquor for preventing and treating alamo grey speck disease germs, and relates to a preparation method of the fermentation liquor. The method mainly solves the technical problems that chemical pesticides are unsafe, and microorganism sourced pesticide is few in kind. The method comprises the steps that (1) excitation cultivation of strains is carried out, (Streptomyces sp.) Syjd3 is shifted to gause first solid media for cultivation to obtain activated (Streptomyces sp.) Syjd3; (2) the activated (Streptomyces sp.) Syjd3 is inoculated to the gause first solid media, the gause first solid media after the inoculation are put into a shake cultivation box for cultivation, and the streptomyces fermentation liquor for preventing and treating the alamo grey speck disease germs is obtained. The streptomyces fermentation liquor has an inhibition function on the alamo grey speck disease germs, and also has certain inhibition functions on alamo leaf blight germs, fusarium solani, wheat root rot germs, curvularia lunata and rice bakanae pathogen.

The invention provides a microbial herbicide for aquatic plant. A bacterial strain is prepared from diphtheria bacillus, curvularia lunata, streptomyces scabies and lactobacillus plantarum.

The invention discloses a Cladosporium sp. strain, and an extract and application thereof. The Cladosporium sp. FS86 is collected to China Center for Type Culture Collection (CCTCC) on March 27th, 2014, wherein the address is Wuhan University, Wuhan City, PRC, and the collection number is CCTCC NO: M2014108. The fermentation extract has obvious inhibitory effects on Candida albicans, Aspergillus niger, Aspergillus flavus, Trichoderma viride, Colletotrichum gloeosporioides, Cylindrocladium scoparium or Curvularia lunata. Therefore, the invention provides scientific references for developing new anti-pathogenic fungus drugs and exploring deep-sea-fungus-derived natural active substances.

The invention discloses streptomyces with an inhibiting effect on poplar gray leaf spot pathogen, relating to a strain of streptomyces. The invention provides the streptomyces which has the inhibiting effect on the poplar gray leaf spot pathogen and provides an effective way for biological prevention and control of poplar gray leaf spot. The streptomyces with the inhibiting effect on the poplar gray leaf spot pathogen is streptomyces Syjd3, and is preserved in the Common microorganism Center of China Committee for Culture Collection of Microorganisms; the preservation address of streptomyces is No. 3, No.1 Institute, west Beichen Road, Chaoyang District, Beijing; the preservation date of streptomyces is June 17, 2014; the preservation number of streptomyces is CGMCC No.9355. Streptomyces Syjd3 disclosed by the invention has the inhibiting effect on the poplar gray leaf spot pathogen, and also has a certain inhibiting effect on poplar stemphylium botryosum, fusarium solani, bipolaris sorokiniana, curvularia lunata and fusarium moniforme.

The invention discloses a novel small peptide IT-9. The amino acid sequence of the peptide IT-9 is ITACVHLPA. A broad-spectrum antifungal activity experiment of the peptide IT-9 shows that hyphae of Bipolaris zeicola and Curvularia lunata growing on a culture medium containing the peptide IT-9 have an abnormal branching phenomenon, but the phenomenon is not observed in a control culture medium without the peptide; it is indicated that the peptide IT-9 can achieve the inhibition effect on Bipolaris zeicola and Curvularia lunata by causing abnormal branches of hyphae. The peptide IT-9 has the highest inhibition rates of 83.6%, 79.8% and 59.2% respectively on Bipolaris zeicola, Curvularia lunata, and Bipolaris maydis.

The invention discloses a peptide KC-19 with antibacterial activity. The amino acid sequence of the peptide KC-19 is KCHLHPLCKGEALELFSVG. A broad-spectrum antifungal activity experiment of the peptide KC-19 shows that the peptide KC-19 achieves an inhibition effect on fungi mainly through hypha aggregation. The highest inhibition rates of the KC-19 on Bipolaris zeicola, Curvularia lunata, Fusarium graminearum are 47.0%, 47.8% and 25.1% respectively.

The invention belongs to the field of crop breeding for disease resistance and relates to an early identification method for curvularia leaf spot in maize inbred lines. The early identification method for curvularia leaf spot in maize inbred lines includes treating leaves of three-leaf seedlings of maize inbred lines in maize curvularia lunata toxin solution, and mixing with sodium borate buffer solution to extract enzyme crude extract of phenylalnine ammonialyase, peroxidase and superoxide dismutase in the leaves; measuring enzyme activities of the phenylalnine ammonialyase, the peroxidase and the superoxide dismutase, and calculating specific activity; calculating resistance values according to a formula, and calculating average of the resistance values. The average larger than 3 is the value of resistance of the inbred line to maize curvularia leaf spot. The method is accurate and reliable. The method can be used to identify and screen indoors without limitations of external environment conditions. In addition, the method has no season limitation in seedling-stage disease resistance identification, and shortens identification time. The method is high in work efficiency.

The invention discloses a compound improvement medicament for preventing common crabgrass herb, and a preparation method thereof. The medicament is prepared from eragrostis pilosa curvularia lunata, colletotrichum, fusarium pachytensis, saccharose, Twain, atrazine, fluazifop p butyl, a wetting agent and a dispersing agent according to a certain proportion. Through the combination of biological andchemical prevention medicaments, the common crabgrass herb in a paddy field can be efficiently inhibited or killed. According to the compound improvement medicament for preventing the common crabgrass herb provided the invention, multiple auxiliaries are adopted, so that a control effect of biological control bacteria is improved, the usage amount of a chemical pesticide is effectively reduced, the drug resistance of the common crabgrass herb is overcome, and the safety on paddy rice is high.

The invention provides microbial flocculant composition and a preparation method thereof, belongs to the field of wastewater treatment and aims to overcome the defects of inorganic and organic flocculants in the prior art. The microbial composite flocculant is prepared from raw materials in parts by weight as follows: 30-40 parts of a co-fermented substance of Curvularia lunata and Rhodococcus erythropolis, 50-95 parts of activated silicate and 3-6 parts of polyoxyethylene. The microbial flocculant composition is high in flocculation activity, safe, non-toxic, free of secondary pollution and suitable for popularization and application.

The invention provides a broad-spectrum functional bacteria (Bacillus simplex)CGMCC No.16458, which has high salt tolerance, multi-function repair and continuous cropping, and salt and alkali resistance for soil resistance. The strain has high ability to dissolve phosphorus, fix nitrogen, produce ferritin, ACCD and cytokinin, and has a salt tolerance of 10%, and has strong antagonistic effects forPythium, Fusarium, Curvularia lunata, Bipolaria maydis and Phytophthora, is suitable for the production of the biological fertilizer with disease prevention, growth promotion, salt and alkali resistance, and the like, and is applied to medium and heavy saline-alkali land production and forage cultivation, as well as from production of vegetables for many years of continuous cropping facilities, the strain promotes emergence, promotes root development, prevents root diseases, improves crop yield and quality, can reduce the use of chemical fertilizers and pesticides, and has broad application prospects.

The invention discloses a functional small peptide KP-11. The amino acid sequence of the peptide KP-11 is KPWLRVALCPG. A broad-spectrum antifungal activity experiment of the peptide KP-11 shows that the peptide KP-11 achieves an inhibition effect on fungi mainly through hypha aggregation. The highest inhibition rates of the peptide KP-11 on corn bihilum, curvularia lunata and maize bihilum are respectively 76.9%, 62.3% and 45.7%.

The invention provides a curvularia lunata steroid C11 beta-hydroxylase mutant L6 and a curvularia lunata steroid C11 beta hydroxylase mutant L7. The DNA sequences are shown as DNA SEQ ID NO: 14 and DNA SEQ ID NO: 16. Compared with C11 beta-hydroxylase CYP5103B6, the specificity of converting steroid substrates 11-deoxycortisol by the mutants L6 and L7 is obviously improved, and byproducts are obviously reduced. The invention further provides an expression vector based on heterologous overexpression of mutants L6 and L7 and a steroid transformation process of the expression vector, and precious materials (genes and strains) and basic data support are provided for researching and developing a process for producing hydrocortisone by efficient C11 beta-hydroxylation 11-deoxycortisol.

The invention belongs to the technical field of biotechnology and steroid drug conversion, and particularly relates to the application of Curvularia crescenae CICC40301 in C14α-hydroxylation. Curvularia lunata (Curvularia lunata) is used for C11β hydroxylation in industry, and the present invention discovers Curvularia lunata with C14α hydroxylation ability for the first time, and Curvularia lunata CICC40301 can transform 4AD into C14α-4AD, yield Reaching 40%‑50%, this discovery will provide a new route for the C14α hydroxylation of steroids.

The invention discloses a bacteria medicament mixture for preventing and controlling weed in a rice field, which is characterized in that: curvularia lunata strain B6 (Wakker) Boedijn with the collection number of CGMGGNO.2928 and cyhalofop-butyl are adopted as active ingredients and proper solvent, surfactant, thickener and stabilizer are adopted as auxiliary materials, and then the active ingredients and the auxiliary materials are subjected to mechanical grinding to prepare a suspension system, wherein the concentration of the active ingredient curvularia lunata strain B6 is (0.1-10)*10<8>spores / ml, and the mixture comprises the following components in percentage by mass: 0.1 to 10 percent of cyhalofop-butyl, 1 to 10 percent of surfactant, 1 to 10 percent of stabilizer, 0.5 to 5 percent of thickener and the balance of solvent. The bacteria medicament mixture is used for preventing and controlling various kinds of weed in the rice field and has the advantages of safety for rice, wide weed controlling range and capability of controlling the weed in the entire growth period of the rice. The invention provides a new herbicide prepared by compounding stable and efficient post-emergence microorganisms with a chemical pesticide, and the new bacteria medicament mixture is environmental-friendly, prevents and controls various kinds of weed in the rice field and obviously increases the yield.

The invention belongs to the technical field of biology, and particularly relates to SSR primers used for analysis of genetic diversity and a genetic relationship of curvularia lunata and application.Nucleotide sequences of the SSR primers used for analysis of the genetic diversity and the genetic relationship of the curvularia lunata are as shown in SEQ ID NO.1-40. The invention further providesa kit used for analysis of the genetic diversity and the genetic relationship of the curvularia lunata. Development of the primers and the kit provides a novel technical method and means for researchand analysis of the genetic diversity and the genetic relationship of the curvularia lunata.

The invention discloses an anti-bacterial protein PBR1 as well as a preparation method and application thereof, and relates to the technical field of molecular biology and biological pesticides. The amino acid sequence of the anti-bacterial protein PBR1 is shown as SEQ ID NO.1. The anti-bacterial protein PBR1 has a superior effect on preventing and curing plant diseases such as cruciferous plasmodiophoromycetes, paddy rice magnaporthe oryzae, curvularia lunata, fusarium oxysporum f. sp. dianthi, fusarium oxysporum vasinfectum, konjac foot rot bacteria, panax notoginseng root rot pathogen, pomegranate wilt pathogen, gaeumannomyces gramini, cylindrosporium pomi brooks, alternaria alternata and / or botrytis cinerea. A pesticide taking the anti-bacterial protein PBR1 as an active ingredient is a novel environment-friendly biological pesticide, is safe to people and livestock, protects the environment, and has a favorable development prospect.

The invention provides a microbial flocculant composition and a preparation method thereof and belongs to the field of wastewater treatment. In order to overcome the shortcomings of an inorganic flocculant and an organic flocculant in the prior art, the biological composite flocculant of the invention comprises the following raw materials, by weight, of 30-40 parts of co-fermentation products of curvularia lunata and rhodococcus erythropolis, 50-95 parts of activated silicate and 3-6 parts of polyoxyethylene. The microbial flocculant composition has the advantages of being high in flocculation activity, safe, non-toxic, free of secondary pollution, and suitable for being applied and popularized.

The invention discloses a Curvularia hominis B-36 and an application of the Curvularia hominis B-36 in synthesis of chiral alcohol. The Curvularia hominis B-36 is preserved in the China Center for Type Culture Collection on November 03, 2017, and the preservation number is CCTCC NO: M2017654. It is found for the first time that Curvularia hominis B-36 has the capacity of catalytic reduction of 1-chloro-2-heptanone, 1-chloro-2-heptanone can be biologically converted into (S)-1-chloro-2-heptanol, the reaction is simple, and the product yield is high.