37 results about "Arthrobacter simplex" patented technology

The invention belongs to the field of microbial pharmaceutics and pharmaceutical engineering, specifically relates to a production method of prednisone acetate by microbial transformation with Arthrobacter simplex as bacteria strain and hydrocortisone acetate as substrate. The method uses Arthrobacter simplex as bacteria strain and comprises the following steps of: performing primary seed culture, performing second fermentation culture, adding hydrocortisone acetate into the fermentation liquid of Arthrobacter simplex to transform hydrocortisone acetate into prednisone acetate, filtering, and collecting cake to obtain prednisone acetate. The bacteria can be prepared into double liquid phase, broken cells or protoplast, each of which has high transformation ratio. The inventive production method replaces cortisone acetate with hydrocortisone acetate as raw material, and has the advantages of high yield, simple process, good economical and practical performance, and less use of harmful reagents; and is important for steroids production with biotransformation method.

The invention relates to application of plasmid capable of being copied in arthrobacter simplex and expressing steroid compound A ring 1, 2 dehydrogenase genes to constructing high-efficiency expression steroid compound A ring 1, 2 dehydrogenation arthrobacter simplex engineering bacteria. The plasmid is capable of being copied in arthrobacter simplex and efficiently expressing steroid compound A ring 1, 2 dehydrogenase genes and significantly practical, and basis is provided for further using plasmid pXJM19 to modify arthrobacter simplex through molecular techniques.

The invention provides a method for preparing 6alpha-medrol (1,4-diene-6alpha-methyl-3,20-diketone-11 beta,17alpha-dihydroxyl pregnane), and is characterized in the following method: adopting 4-alkene-6alpha-methyl-3,20-diketone-11 beta,17alpha-dihydroxyl pregnane as the substrate, fermentation enzyme solution obtained from the fermentation of Arthrobacter simplex as enzyme source, adding 0.01-0.08g / 100mL fermentation enzyme solution vitamin K3, carrying out a converting reaction at 25-35DEG C for 36-60h till complete reaction, separating and purifying to obtain the final product1,4-diene-6alpha-methyl-3,20-diketone-11 beta,17alpha-dihydroxyl pregnane. The beneficial effects of the biological preparation method of 1,4-diene-6alpha-methyl-3,20-diketone-11 beta,17alpha-dihydroxyl pregnane is mainly reflected in: (1) wide raw materials sources, and low cost, (2) good selectivity, simple process, industrial production, (3) mild reaction conditions, easy separation, highly pure product.

The invention relates to a method for directly generating prednisolone through bio-fermentation in one step by using hydrocortisone acetate as a raw material and Arthrobacter Simple ATCC 21032.

The invention discloses a method for preparing 16alpha, 17alpha-epoxy-11alpha-hydroxy-pregna-1,4-diene-3,20-dione by taking 16alpha,17alpha-epoxy progesterone as a starting material through one-step mixed fermentation of aspergillus ochraceus and arthrobacter simplex.

The invention provides a method for preparing 11beta-hydroxyl-1,4-diene-3,20-dione steroid through co-fermentation of curvularia lunata and arthrobacterium, relating to a method for preparing a compound II by adopting 4-alkene-3,20-dione steroid I as an initiator through microbial co-fermentation. The method is characterized in that the 11beta-hydroxyl-1,4-diene-3,20-dione steroid II is prepared through the mixed one-step fermentation of curvularia lunata AS 3.4381 and arthrobacter simplex AS1.94.

The invention discloses a method for preparing a dexamethasone epoxy hydrolysate 8DM from arthrobacter simple CPCC140451 through one-step fermentation. The method is simple in operation manner, stablein process and suitable for industrial mass production.

The invention discloses a method for preparing delta 1-11 alpha,17 alpha-dihydroxyprogesterone with a one-pot continuous fermentation process, and belongs to the technical field of intermediate preparation. The method comprises the following specific steps: (1) inoculating slant culture of arthrobacter simplex into a first culture medium, performing culture for 20-30 hours, adding ground 17 alpha-hydroxyprogesterone, and performing transformation; (2) inoculating fermentation liquor after finishing the step (1) into a second culture medium, inoculating slant culture of aspergillus ochraceus into the second culture medium, performing culture, and performing post-treatment to obtain delta 1-11 alpha,17 alpha-dihydroxyprogesterone. Delta 1-11 alpha,17 alpha-dihydroxyprogesterone is prepared with the one-pot continuous fermentation process, so that a step of filtering, extracting, refining and drying a dehydrogenation material is reduced, discharge of 50% pollutants is reduced, environmental pressure is reduced, the time is saved, the yield is increased, the efficiency is improved, the process is simplified, the whole reaction operation is convenient, and the method is suitable for industrialized popularization and application.

The invention discloses a method for preparing 16,17[alpha]-epoxyprogesterone by virtue of a biological process. By conducting biological fermentation on a 3-hydro-5-ene-16,17[alpha]-epoxy substance by virtue of autologous cholesterol oxidase of Arthrobacter simplex AS1.94, so that biological manufacturing of the 16,17[alpha]-epoxyprogesterone is achieved; therefore, a conventional chemical process is replaced and the conversion rate and yield of the 16,17[alpha]-epoxyprogesterone are improved, wherein the 3-hydro-5-ene-16,17[alpha]-epoxy substance serves as a substrate and the Arthrobacter simplex AS1.94 is a catalysis strain; and by conducting the fermentation, the yield of the 16,17[alpha]-epoxyprogesterone is greater than 90%, and the purity of the 16,17[alpha]-epoxyprogesterone is greater than 98.5%.

The invention relates to application of plasmid capable of being copied in arthrobacter simplex and expressing steroid compound A ring 1, 2 dehydrogenase genes to constructing high-efficiency expression steroid compound A ring 1, 2 dehydrogenation arthrobacter simplex engineering bacteria. The plasmid is capable of being copied in arthrobacter simplex and efficiently expressing steroid compound A ring 1, 2 dehydrogenase genes and significantly practical, and basis is provided for further using plasmid pXJM19 to modify arthrobacter simplex through molecular techniques.

The invention belongs to the field of genetic breeding, and in particular relates to a simple Arthrobacter mutant strain with stress tolerance and a genetic engineering bacterium constructed therefrom. Among them, the survival rate of the mutant strains under 16% ethanol and 20% methanol shock conditions increased by 3.74 times and 2.10 times respectively compared with the starting strain; overexpression of groEL, dnaK, recA, uvrD, katG, sod or treS genes from the mutant strains The tolerance of the engineered bacteria to high concentrations of organic solvents is significantly improved, and they also show higher tolerance to salt stress and oxidative stress. The above engineered bacteria are used in the steroid C1,2 dehydrogenation reaction to put The ethanol concentration was increased to 8-10%, the substrate CA concentration was increased to 6-8g / L, and the product yield was increased by 0.41-3.56 times, which laid a good foundation for the subsequent research on the resistance mechanism and the molecular transformation and application of strains.

The invention provides a method for preparing a betamethasone intermediate. By using one of a single strain Arthrobacter simplex CPCC 140451 or a single strain Nocardioide simplex ACCC 10205, the hydrolysis and dehydrogenation processes of the compound I are conducted in a same system, and it is achieved that beta methyl epoxide is obtained through one-step fermentable conversion. The technology is simple, and the method is suitable for industrial production.

The invention belongs to the field of microbial pharmaceutics and pharmaceutical engineering, specifically relates to a production method of prednisone acetate by microbial transformation with Arthrobacter simplex as bacteria strain and hydrocortisone acetate as substrate. The method uses Arthrobacter simplex as bacteria strain and comprises the following steps of: performing primary seed culture, performing second fermentation culture, adding hydrocortisone acetate into the fermentation liquid of Arthrobacter simplex to transform hydrocortisone acetate into prednisone acetate, filtering, andcollecting cake to obtain prednisone acetate. The bacteria can be prepared into double liquid phase, broken cells or protoplast, each of which has high transformation ratio. The inventive production method replaces cortisone acetate with hydrocortisone acetate as raw material, and has the advantages of high yield, simple process, good economical and practical performance, and less use of harmful reagents; and is important for steroids production with biotransformation method.

The invention provides a method for preparing a betamethasone intermediate. By using one of a single strain Arthrobacter simplex CPCC 140451 or a single strain Nocardioide simplex ACCC 10205, the hydrolysis and dehydrogenation processes of the compound I are conducted in a same system, and it is achieved that beta methyl epoxide is obtained through one-step fermentable conversion. The technology is simple, and the method is suitable for industrial production.