The invention relates to a preparation and pre-use treatment method for glycolonitrile with cyanide-containing tail gas as the raw material. According to the method, the cyanide-containing tail gas is introduced into multiple stages of reaction stills containing formaldehyde and a catalyst to be directly subjected to an addition reaction, and a glycolonitrile product with a certain concentration is obtained; after material transferring, the glycolonitrile product is stored under the effect of a stabilizer for use, and before use, the pH is regulated with base or weak base till the glycolonitrile product is neutral. The catalyst is one of basic compounds including Na2SO3, Na2CO3, NaHCO3 and NaOH, the mass concentration of formaldehyde is 35%-37%, the mol ratio of formaldehyde to the catalyst is 1:(0.001-0.05), and the reaction temperature is 5-20 DEG C. According to the method, HCN in industrial waste gas of a steroid drug is chemically absorbed through formaldehyde, the catalyst and the stabilizer, glycolonitrile is generated through the reaction and serves as a raw material for biological preparation of glycollic acid, and therefore the cyanide-containing tail gas is fully absorbed and utilized and prevented from being directly exhausted to the atmosphere and polluting the environment.