105 results about "Stigmasterol" patented technology

This invention publishes a phytosterol extract from bamboo shoots, its preparation method and application. The phytosterol extract from bamboo shoots mentioned in this invention contains 5~50% sterols, 10~40% of which is beta-sitosterol. The ratio between beta-sitosterol, stigmasterol and campesterol is 10~40:1~3:2~5. The phytosterol extract from bamboo shoots mentioned in this invention performs significant anti-inflammatory and leukemia histiocytosis inhibitory activities and is thus applicable in cosmetics, foods, health products and medicines.

The invention discloses a method for simultaneously determining stigmasterol, campesterol, beta-sitosterol and squalene in vegetable oil. The method comprises the following steps of: 1) saponifying the vegetable oil, and extracting an unsaponifiable matter in a saponification solution by using an organic solvent; and 2) dissolving the unsaponifiable matter in the organic solvent, and determining by using high-performance liquid chromatography, wherein the high-performance liquid chromatography has the chromatographic conditions that a chromatographic column is a C18 column, a mobile phase is a solvent of which the volume ratio of acetonitrile to water is (90:10) to (100:0), an ultraviolet detection wavelength is 205 to 210 nm, a sample size is 10 to 20 mu L, flow speed is 1.0 to 1.5 mL / min and column temperature is 25 to 30 DEG C. By adoption of the method, effective separation of the campesterol, the stigmasterol, the beta-sitosterol and the squalene in the vegetable oil can be simultaneously realized, consumed time for separation is about 40 min, and components in a chromatogram are eluted in a sequence of the campesterol, the stigmasterol, the beta-sitosterol and the squalene.

The stigmasterol extracting process from mixed plant sterol is one stigmasterol enriching solvent process including the steps of water bath dissolution at 70-75 deg.c, cooling to 20-25 deg.c to crystallize for 12-24 hr, and heat insulating suction filtering. Mixed solvent is used including n-heptane and dichloroethane in the radio of 1 to 1-2, preferably 1 to 1.5-1.8. The ratio between mixed plant sterol and the solvent is 1 to 3-5, preferably 1 to 3.5-4.5. The said process is performed repeatedly for high stigmasterol content and the filtrate is low temperature treated at -10 deg.c to recover sterol and solvent.

A novel use is disclosed of liposomes that contain phytosterol (e.g., β-sitosterol, campesterol, stigmasterol, brassicasterol, ergosterol, and ergostadienol, and so forth). It has been newly discovered that liposomes that contain phytosterol have a cancer metastasis inhibiting activity. Cancer metastasis is inhibited by the oral administration of liposomes that contain phytosterol and these liposomes are therefore useful for the prevention and treatment of cancer.

Disclosed are: a fat-reduced soymilk having a reduced fat content, which is produced by separating a fat from a fat-containing soybean efficiently without relying on an organic solvent; and a novel fat-rich soybean material. Specifically disclosed are: a fat-reduced soybean protein material characterized by containing a protein and a carbohydrate at a total content of 80 wt % or more in terms of dried form content, containing a fat (as an extract from a chloroform / methanol mixed solvent) at a content of less than 10 wt % relative to the content of the protein, and containing campesterol and stigmasterol (as plant-derived sterols) at a total content of 200 mg or more relative to 100 g of a fat; and a soybean emulsion composition characterized by containing a protein at a content of 25 wt % or more in terms of dried form content, containing a fat at a content of 100 wt % or more in terms of dried form content relative to the content of the protein in terms of dried form content, and having an LCI value of 60% or more.

The invention belongs to the field of daily necessities and particularly relates to folium artemisiae argyi anti-mosquito lotion and a preparation method thereof. The folium artemisiae argyi anti-mosquito lotion consists of the following components in parts by weight: 1-3 parts of plant essential oil, 1-3 parts of a solubilizer, 5-12 parts of low alcohol, 0.1-0.2 part of decanedioic acid, 0.1-0.5 part of a preservative and 40-60 parts of water, wherein the solubilizer consists of PEG-40 hydrogenated castor oil and stigmasterol in a weight ratio of 1:(0.1-0.8). The anti-mosquito lotion provided by the invention has the advantages of excellent anti-mosquito effect, stable quality, long anti-mosquito time, safety, environmental friendliness, good using effect and the like; with low ethanol content, the anti-mosquito lotion generates little irritation to a human body and is widely suitable for people; and moreover, the preparation technology is simple, the production cost is low, and the anti-mosquito lotion is suitable for vigorous promotion and application.

The process of separating and purifying stigmasterol from plant sterol includes: the first step of adding solvent absolute ethyl alcohol, n-propanol or n-butanol, heating to dissolve, slowly lowering temperature to crystallize, filtering, centrifugally separating and vacuum drying; and the second step of adding separating solvent into the product of the first step, heating to dissolve, slowly lowering temperature to crystallize and filtering; with the separation capable of being repeated for 2 or 3 times. The present invention has separate material concentration and separation refining steps, and has greatly raised stigmasterol purity and yield and no environmental pollution.

The invention discloses a method for extracting stigmasterol from coix stems. The method comprises the following steps: enzymatic hydrolysis; ultrasonic extraction; saponification; urea column chromatography; crystallization; etc. The method provided by the invention can retain the natural characteristics of stigmasterol and has the advantages of few process steps, mild operation conditions, low solvent toxicity, safe production process and high extraction efficiency; and the extracted stigmasterol has a purity as high as 99%.

The invention discloses a sterol derivative of beta-sitosterol, beta-stigmasterol and cholesterol, and is shown as a formula VI. The invention also discloses a preparation method of the sterol derivative. The invention also discloses application of the sterol derivative to the aspect of preparation of wound healing promoting medicine. By starting from easily obtained natural products, the beta-sitosterol, the beta-stigmasterol and the cholesterol are used as starting raw materials; the synthetic method is simple; better operability and reaction yield are realized. The prepared sterol derivative has the obvious wound healing promoting activity; the multiplication, migration and collagen synthesis capability on L929 mechanocytes is obviously higher than that of the raw material and positive control medicine recombinant human bFGF (basic fibroblast growth factor). Compared with protide type medicine (such as bFGF), the prepared sterol derivative has more diversified dosage forms and medication modes; the reference is provided for the application in the field of wound healing promoting. The formula VI is shown as the accompanying diagram.

The present invention addresses the problem of providing a soybean-derived raw material-containing food or beverage that improves problems with flavor and physical properties, such as the grassy smell caused by soybean raw materials, and markedly improves product quality, in a soybean-derived raw material-containing food or beverage using a conventional soybean raw material such soymilk or tofu. Provided are a milk-substitute composition, an egg yolk-substitute composition, and a composition for improving renal function, etc., characterized by including a reduced-fat soybean protein material having a total protein and carbohydrate content relative to dry material of at least 80 wt %, a fat content (as a chloroform / methanol mixed solvent extract) relative to the protein content of less than 10 wt %, and a total campesterol and stigmasterol (as plant-derived sterols) content of at least 200 mg relative to 100 g of fat. Also provided are a variety of soybean-derived raw material-containing food and beverages using these compositions.

A method for separating beta-sitosterol and stigmasterol from mixed phytosterol comprises the following steps: (1) mixing and dissolving the mixed phytosterol and n-amyl alcohol at 60-70 DEG C, adding the stigmasterol to prepare a saturated solution of the stigmasterol at the temperature, and thermally filtering the solution to remove impurities so as to obtain a filter liquor; (2) cooling the filter liquor to 20-30 DEG C, stirring at a constant temperature, maintaining the temperature, and filtering to obtain a filter cake of crude stigmasterol; (3) cooling the filtrate to 0-10 DEG C, stirring at a constant temperature, maintaining the temperature, and filtering to obtain a filter cake of crude beta-sitosterol; (4) refining the crude stigmasterol with the n-amyl alcohol to obtain a stigmasterol product with high purity; (5) refining the crude beta-sitosterol with the n-amyl alcohol to obtain a beta-sitosterol product with high purity. The method is simple in process step and safe in production; the solvent is simplex, small in using amount, and easy to recycle; the products are high in purity and high in yield; the method is applicable to industrial production.

Compositions and methods for urea silicone gels for treating hyperkeratotic skin diseases that may benefit from barrier protection and from urea silicone gel's ability to return water balance to the skin are disclosed. Skin conditions that may be treated with urea silicone gels may be excessive dryness, callus, corns, old and new scars, and keloids, among others. Disclosed urea silicone gels may include micronized urea USP, and an anhydrous silicone base, among other ingredients. Anhydrous silicone base may include Amazonian oils such as pracaxi oil and seje oil, which are rich in oleic, linolenic, linoleic acids, and sterols, particularly beta-sitosterol and stigmasterol, which may increase skin permeability to urea. Additionally, because of the high content of unsaturated fatty acids of pracaxi oil and seje oil, disclosed urea silicone gel may exhibit an enhanced hydrating effect on skin cells, over-moisturizing and hydrating tough, thick hyperkeratotic conditions.

The present invention provides a cyclic cholane carboxylate derivative and a preparation method thereof, and uses of the cyclic cholane carboxylate derivative in industrial preparation of 25-hydroxycholesterol (1), wherein R1 in the cyclic cholane carboxylate derivative represented by a formula I is straight chain or branched chain C1-C12 alkyl, R2 is methyl or ethyl, and the formula (I) is defined in the specification. According to the present invention, the starting raw material stigmasterol of the 25-hydroxycholesterol (1) synthesis process route has characteristics of low price, easy obtaining and reliable commercial source; and particularly during the constructing of the 17-position side chain of 25-hydroxycholesterol (1), the reaction conditions are mild, the reaction steps are simple, the special and dangerous reagents are not required, the harsh reaction conditions are not required, the redundant reaction steps are not required, the chromatographic separation and purification is not required, the industrial production is easily achieved, and the significant progress is achieved compared to the prior art.

A catalytic hydrogenation preparation of high content beta - (citrostadienol ) sitosterol used in food, cosmetic and steroid drugs etc. is carried out by: mixing plant sterol materials, dissolving in organic solvent, catalytic hydrogenating with hydrogenated catalysts under normal temperature and pressure or at certain pressure, hydrogenising stigmasterol in mixed plant sterol to get beta -sitosterol, finally, obtaining liquid phase product with 60% - 75% content of beta -sitosterol and higher yields. The loss of sterol is bellow 10%. Its advantages include: operating simply and safely with simple equipment, low production cost, clean environment.

A method for segmented purification of beta-sitosterol and stigmasterol by using n-amyl alcohol comprises the following steps: (1) mixing and dissolving blended phytosterol and n-amyl alcohol at the temperature of 60-70 DEG C, then adding beta-sitosterol to prepare a beta-sitosterol saturated solution at the temperature, thermally filtering, cooling a filter liquor, stirring at a constant temperature, preserving heat and filtering to obtain a crude product of beta-sitosterol; (2) continuing to cooling the filter liquor, stirring at the constant temperature, preserving heat and filtering to obtain a crude product of blended sterol; (3) heating and dissolving the crude product of blended sterol by using the n-amyl alcohol to prepare a stigmasterol saturated solution, cooling, stirring at the constant temperature, preserving heat and filtering to obtain a crude product of stigmasterol; (4) heating and concentrating the filter liquors in the step (2) and the step (3), cooling, stirring at the constant temperature, preserving heat and filtering to obtain the crude product of beta-sitosterol; (5) refining the crude product of stigmasterol by using the n-amyl alcohol; (6) refining the crude product of beta-sitosterol by using the n-amyl alcohol. The method provided by the invention is simple in steps, a single solvent is easy to recover and does not cause pollution, and the product purity and the yield are high.

The invention provides a method for preparing sitostamol from stigmasterol through catalyzed hydrogenation, wherein organic solvent is used as reaction medium, palladium / carbon is used as catalyst for catalyzing hydrogenation of stigmasterol under atmosphere pressure or low pressure. The method can lower the preparation cost of sitosterane alcohols.

The invention relates to the use of stigmasterol in the manufacture of a nutritional or pharmaceutical composition for prophylactic or therapeutic treatment of amyloidosis of beta-amyloid peptide (Ab) in a mammal. Further, the invention relates to the use of stigmasterol in the manufacture of a nutritional or pharmaceutical composition for prophylactic or therapeutic treatment of Alzheimers disease.

The invention discloses a method for preparing high-purity stigmasterol from phytosterol oleate. The method comprises the following steps of taking phytosterol oleate as a raw material, and performingsubzero fractionation, saponification, solid-liquid separation and two times of crystallization and purification to obtain a stigmasterol product with purity equal to 95% or more. Compared with the prior art, in the method, the phytosterol oleate is taken as the raw material to prepare the high-purity stigmasterol, the method has fewer crystal number of times and is simple in operation steps, thesolvent used for crystallization and purification can be recycled, making full use of resources is realized, and the production cost is lowered.

The invention relates to foliar fertilizer for inducing melon vegetable powdery mildew resistance and a preparation method thereof. The fertilizer is prepared from the following raw materials: salicylic acid, methyl jasmonate, chitooligo saccharide, stigmasterol, earthworm hydrolysate, a new high fat film, betaine, a wetting agent, a dispersant and water. The preparation method comprises the following steps of preparing the earthworm hydrolysate; adding water into a reaction kettle, heating to 40 to 50 DEG C, adding the salicylic acid and the wetting agent, and stirring to react to obtain a solution A; after the solution is cooled to 30 to 40 DEG C, adding the stigmasterol, the chitooligo saccharide and the dispersant, stirring to react to obtain a solution B; uniformly mixing the earthworm hydrolysate, the solution A, the solution B and the remaining ingredients, sterilizing, and filling, so as to obtain the foliar fertilizer of the invention. According to the foliar fertilizer of the invention, by inducing the resistance of melon vegetables to powdery mildew, the pollution caused by chemical agents can be remarkably reduced, and food safety can be improved, the foliar fertilizer also can be used as an important complement to the application of disease-resistant varieties, can significantly promote the growth of melon vegetables and improve quality and increase yield, and is an effective way to control powdery mildew.

The invention discloses a method for detecting nine nutrients in edible vegetable oil. The method comprises the following steps: (1) dissolving the edible vegetable oil into a mixed solvent of cyclohexane and ethyl acetate, so as to obtain an edible vegetable oil solution, wherein the volume ratio of cyclohexane to ethyl acetate is 1 to 1; (2) injecting the edible vegetable oil solution prepared in the step (1) into a preparation type gel permeation chromatographic instrument, and dissolving residue of the blow-dried effluent, and filtering to obtain a target analyzing solution, wherein the purification conditions are that a gel chromatographic column is 300mm*20mm, the feeding volume is 5mL, the mixed solvent of cyclohexane and ethyl acetate serves as a moving phase, the flow speed is 4.7mL per minute, the collection time of effluent is from seven forty to fifteen o'clock; and (3) injecting the target analyzing solution obtained in the step (2) into a liquid chromatogram-mass spectrometer, measuring, and analyzing the contents of the nine nutrients, namely erythrodiol, arbutin, ergosterol, brassicasterol, campesterol, fucosterol, stigmasterol, cholesterin and beta-sitosterol by virtue of an external standard method.

The invention discloses a synthesis method of 28-homobrassinolide, which comprises the steps of selecting stigmasterol B as an initial raw material, carrying out esterification reaction to obtain a compound C, and heating and refluxing the compound C under an alkaline condition to obtain a compound D; oxidizing the compound D to obtain a compound E; performing oxidation and ring opening on the compound E to obtain a compound F; carrying out a dihydroxylation reaction on the compound F to obtain a compound G; and carrying out oxidation rearrangement reaction on the compound G to obtain a compound A 28-homobrassinolide. The brand new synthesis of 28-homobrassinolide is realized through a simple six-step synthesis process including esterification reaction, ring-closing reaction, oxidation reaction, oxidation ring-opening reaction, dihydroxylation reaction and oxidation rearrangement reaction, and the preparation method has the advantages of easily available raw materials, short synthesis route, relatively ideal yield, low cost, low toxicity, low environmental pollution and the like, has a high industrial production value and a good agricultural application prospect, and provides a new path for synthesis of a steroid plant growth regulator 28-homobrassinolide substance.

The invention provides a novel industrial method for preparing vitamin D3 by taking stigmasterol as a raw material. The method comprises the following steps: sequentially carrying out hydroxyl acetylation, side chain oxidation, side chain isopentane reduction and hydrogenation on stigmasterol to obtain cholesterol acetate, and then sequentially carrying out oxidation, hydrazone formation, hydrazone removal, hydrolysis, illumination and the like to obtain the vitamin D3. The invention provides a novel method for preparing vitamin D3 from stigmasterol, and the method has the advantages of mild reaction conditions and high yield, and is suitable for industrial production.

The invention discloses a multi-stimulation responsive stigmasterol derivative low-molecular-mass gelator, organic gel and preparation methods of the low-molecular-mass gelator and the organic gel. The stigmasterol-based glycine ferrocene amide gelator is prepared through a three-step synthesis reaction including esterification, BOC removal and amidation for the first time with stigmasterol with arigid framework used as a gelator construction parent, glycine used as a linking arm and ferrocene containing an oxidation activity group as a reaction group, and the problem of lack of research on construction of a super-molecular gel system with stigmasterol and ferrocene derivatives as gelators in the prior art is solved. An organic gel material of the gelator has properties of thixotropy, ultrasonic response, thermal response and alcohol solvent stimulation response besides oxidation stimulation response expected to be obtained through introduction of the ferrocene group, and the gel is expected to have potential value in the aspects of sensors, solvent recognition and the like.

A process for isolating at least one target compound, such as manool, geranyl linalool, ethyl guaiacol, eugenol, veratraldehyde, squalene, terpin, cholesterol, beta-sitosterol, campesterol, stigmasterol, stigmastenol and dehydroabietic acid, from biomass, the process including steps of: obtaining a condensate from a recovery evaporator, a reverse osmosis retentate of a condensate of a pulp and paper mill, or both, the condensate, retentate or both being substantially free of higher molecular weight (approximately >1000 Da) cellulose and / or lignin and / or lignin-derived material; optionally pH adjusting and filtering the condensate to collect insoluble material; extracting the condensate, the collected insoluble material, or both, with solid phase extraction (SPE), liquid-liquid extraction or solid-liquid extraction to produce an extract containing the at least one target compound; and optionally purifying the extract containing the at least one target compound by thermal fractionation, chromatographic separation, recrystallization ion exchange, chelation, adsorption / desorption, lyophilization and sublimation or combinations thereof. The method is particularly useful for isolating the target compounds from wastewaters produced in a kraft pulp and paper mill, especially from recovery evaporator condensates produced during the treatment of black liquor.

The invention relates to a preparation method for a progesterone intermediate. According to the method the progesterone intermediate III is prepared from a compound I through two steps of calcium hypochlorite oxidation and chromic anhydride oxidation. And the product progesterone intermediate III can be synthesized into progesterone through two steps of reaction. The reaction route is shown in the specification. According to the method, stigmasterol is taken as raw material, and the method has the characteristics that the resources are more abundant, the synthesis time is short, the efficiency is high, and the synthesized intermediate is high in purity.

The invention discloses a pharmaceutical composition for treating diabetic retinopathy and a preparation method thereof. The pharmaceutical composition for treating diabetic retinopathy consists of the following components in parts by weight: coarse grain mixed powder, pseudolaric acid, p-chlorophenoxy isobutyric acid, syringin, phycocyanobilin, friedelin, stigmasterol, formononetin, L-alpha-aminoadipic acid, pancreatin, L-malic acid, vitamin and L-glutamic acid. The pharmaceutical composition disclosed by the invention realizes the effects of controlling blood glucose and treating retinopathy by improving glycometabolism, focusing on blood glucose reduction assisted by the medicinal components for nourishing yin and clearing heat and resolving stasis and by improving the human immunity to enhance the tolerance and resistance against lesion; and moreover, the pharmaceutical composition is simple in technology, realizes a good curative effect and can be promoted for application.