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Method for extracting stigmasterol from coix stems

A technology of stigmasterol and coix seed, applied in the field of production and preparation of phytosterol, can solve the problems of many production wastes, low sterol yield, large solvent consumption, etc., and achieves the effects of low cost, high extraction efficiency and shortened extraction time.

Active Publication Date: 2017-02-08
西安飞达生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method consumes a lot of solvent, produces a lot of waste, consumes a lot of energy, and has a low yield of sterols.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for extracting stigmasterol from coix stems, comprising the following steps:

[0028] (1) Enzymatic hydrolysis: put coix stem into a pulverizer to obtain coix stem coarse powder, add cellulase and pectinase to the coix stem coarse powder, then dissolve in water of 1 times the weight of the coarse powder, at 50°C Under enzymolysis for 8h, filter, and dry to obtain enzymolysis Coix stem powder; wherein the weight of cellulase and pectinase added is 0.1% of the weight of Coix stem meal weight, and the weight ratio of cellulase and pectinase is 2: 1;

[0029] (2) Ultrasonic extraction: add enzymatic coix stem powder and absolute ethanol to ultrasonic equipment for double ultrasonic extraction, the ratio of solid to liquid between enzymatic coix stem powder and absolute ethanol is 1:10, and the frequency of the two ultrasonic waves is 20kHz and 40kHz, the power is 50W, 100W, and the extraction time is 20min; filter to obtain the extract, put the extract into a rota...

Embodiment 2

[0035] A method for extracting stigmasterol from coix stems, comprising the following steps:

[0036] (1) Enzymatic hydrolysis: put coix stem into a pulverizer to get coix stem coarse powder, add cellulase and pectinase to the coix stem coarse powder, then dissolve in water three times the weight of the coarse powder, at 30°C Under enzymatic hydrolysis for 5h, filter, and dry to obtain enzymolysis coix stem powder; wherein the weight of cellulase and pectinase added is 1% of the weight of coix stem meal weight, and the weight ratio of cellulase and pectinase is 2: 1;

[0037] (2) Ultrasonic extraction: Add enzymatic coix stem powder and absolute ethanol to ultrasonic equipment for triple-frequency ultrasonic extraction, the ratio of solid to liquid between enzymatic coix stem powder and absolute ethanol is 1:15, and the frequency of the three ultrasonic waves is 40kHz , 60kHz, 1000kHz, the power is 200W, 500W, 2000W, and the extraction time is 10min; filter to obtain the extr...

Embodiment 3

[0043] A method for extracting stigmasterol from coix stems, comprising the following steps:

[0044] (1) Enzymatic hydrolysis: put coix stem into a pulverizer to get coix stem coarse powder, add cellulase and pectinase to the coix stem coarse powder, then dissolve in water twice the weight of the coarse powder, at 40°C Under enzymolysis for 6h, filter, and dry to obtain enzymolysis Coix stem powder; wherein the weight of cellulase and pectinase added is 0.5% of the weight of Coix stem meal weight, and the weight ratio of cellulase and pectinase is 1: 1;

[0045] (2) Ultrasonic extraction: add enzymatic coix stem powder and absolute ethanol to ultrasonic equipment for dual-frequency ultrasonic extraction, the ratio of solid to liquid between enzymatic coix stem powder and absolute ethanol is 1:12, and the frequency of the two ultrasonic waves is 35kHz and 55kHz, the power is 100W, 200W, and the extraction time is 30min; filter to obtain the extract, put the extract into a rot...

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PUM

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Abstract

The invention discloses a method for extracting stigmasterol from coix stems. The method comprises the following steps: enzymatic hydrolysis; ultrasonic extraction; saponification; urea column chromatography; crystallization; etc. The method provided by the invention can retain the natural characteristics of stigmasterol and has the advantages of few process steps, mild operation conditions, low solvent toxicity, safe production process and high extraction efficiency; and the extracted stigmasterol has a purity as high as 99%.

Description

technical field [0001] The invention relates to the field of production and preparation of phytosterols, in particular to a method for extracting stigmasterol from coix stems. Background technique [0002] Stigmasterol (stigmaster01), C 29 h 450 . The molecular weight is 412, also known as stigmaster-5,22-diene-3B-alcohol or 24R-ethylcholesteric-5,22-diene-3B-alcohol, which is a kind of phytosterol widely present in various in plants. Stigmasterol has significant anti-oxidation, anti-tumor, anti-cardiovascular disease and other pharmacological activities. It is also the starting material for the synthesis of steroid drugs and has applications in medicine, food and chemical industries. In recent years, with the discovery of new steroidal drugs, the development of production, and the expansion of production scale, the demand for raw materials has gradually increased; There is a contradiction between supply and demand, how to obtain more and higher purity stigmasterol is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00
CPCC07J9/00
Inventor 黄锁义秦建鲜廖献就王露瑶黄上峰梁凌玲潘乔丹喻巧容
Owner 西安飞达生物技术有限公司
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