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82 results about "Campesterol" patented technology

Campesterol is a phytosterol whose chemical structure is similar to that of cholesterol, and is one of the ingredients for E number E499.

Process for the preparation of high purity phytosterols from deodourizer distillate from vegetable oils

The present invention provides an isolation process of the pure sterols from the SODD by simple acid catalyzed esterification and the separation of the reagents by distillation and water washing followed by the crystallization of phytosterols and purification. Soybean oil deodorizer distillate (SODD) and distillate from other vegetable oil refining contain free sterols, in addition to steryl esters, tocopherols squalene and unknown compounds. The process is also applicable to other vegetable oil distillate containing more than 1% phytosterols in the free form. Phytosterols thus obtained is of high purity (95-99%) and high yield (80-90%) and contains β-sitosterol, Stigmasterol and campesterol.
Owner:COUNCIL OF SCI & IND RES

Food additive for cleaning and fresh keeping

The invention provides a food additive for cleaning and keeping fresh, which comprises the following ingredients in parts by weight: 0.2-18% of ethyl maltol, 3-12% of eucalyptus essential oil, 20-48% of Sapinberry fruit peel extract, sodium bicarbonate 1-6%, 0.01-10% of pothos radix extract, 3-5% of campesterol, 1-4% of edible salt, 5-15 parts of sodium glycinate, 4-8 parts of glutamic acid, 4-8 parts of aloe extract, 4-16 parts of wheat starch, the rest is water, when the food additive used for cleaning and fresh-keeping fruits and vegetables of the present invention cleans and keeps fresh fruits and vegetables, it can remove the insufficiency of the original chemical cleaning agent cleaning, and can effectively avoid food pollution. Its composition is food grade, High safety factor.
Owner:王俊

Recombinant yarrowia lipolytica T30pED with high yield of campesterol as well as construction method and application of recombinant yarrowia lipolytica T30pED

ActiveCN113604470AIncrease reductase activityMicroorganism based processesPeptidesEngineeringBiochemistry
The invention relates to recombinant yarrowia lipolytica T30pED with high yield of campesterol as well as a construction method and application of the recombinant yarrowia lipolytica T30pED. The strong promoter TEF30 provided by the invention can be used for constructing a recombinant strain of the yarrowia lipolytica, and verification shows that the highest yield of the campesterol in shake flask fermentation reaches 664.1 mg / L (33.21 mg / g DCW) and the highest yield of the campesterol in a 5L fermentation tank reaches 1305.2 mg / L (68.73 mg / g DCW), which are obviously higher than the yield of a wild type promoter strain, so that the strong promoter has outstanding industrial application significance.
Owner:SHAANXI HEALTHFUL BIOLOGICAL ENG

Process for refining chemicals from pulp and paper mill wastewaters

A process for isolating at least one target compound, such as manool, geranyl linalool, ethyl guaiacol, eugenol, veratraldehyde, squalene, terpin, cholesterol, beta-sitosterol, campesterol, stigmasterol, stigmastenol and dehydroabietic acid, from biomass, the process including steps of: obtaining a condensate from a recovery evaporator, a reverse osmosis retentate of a condensate of a pulp and paper mill, or both, the condensate, retentate or both being substantially free of higher molecular weight (approximately >1000 Da) cellulose and / or lignin and / or lignin-derived material; optionally pH adjusting and filtering the condensate to collect insoluble material; extracting the condensate, the collected insoluble material, or both, with solid phase extraction (SPE), liquid-liquid extraction or solid-liquid extraction to produce an extract containing the at least one target compound; and optionally purifying the extract containing the at least one target compound by thermal fractionation, chromatographic separation, recrystallization ion exchange, chelation, adsorption / desorption, lyophilization and sublimation or combinations thereof. The method is particularly useful for isolating the target compounds from wastewaters produced in a kraft pulp and paper mill, especially from recovery evaporator condensates produced during the treatment of black liquor.
Owner:HER MAJESTY THE QUEEN & RIGHT OF CANADA

Method for preparing five ferulate single components by taking soapstock containing oryzanol as raw material

The invention provides a method for preparing five ferulate single components by taking soapstock containing oryzanol as a raw material. The method comprises the following steps: 1) carrying out alkali-alcohol hot dissolution and filtering while the soapstock is hot; 2) carrying out acid precipitation and filtration; 3) degreasing and decoloring by using a lipophilic organic solvent; and (4) preparing thin-layer chromatography for separation; wherein the condition is that the content of the oryzanol in a precipitate crude product obtained after decoloring in the step (3) is 70-80wt%. The method is characterized in that soapstock with the oryzanol content of 10-20% is used as a raw material, a product with the oryzanol content of 70-80 wt% is obtained through a specific pretreatment purifying step, and then a monomer substance is separated and prepared through thin-layer chromatography; the purity of the five prepared monomers, namely 24-methylene cycloartanyl ferulate, cycloartenyl ferulate, cycloartanyl ferulate, campesterol ferulate and beta-sitosterol ferulate is greater than 90%; and the ferulate single components can be used as a reference substance for content determination,and can be used for external standard method content calculation and qualitative peak position calibration.
Owner:HUNAN HUACHENG BIOTECH

Camellia oleosa seed oil adulteration detection method based on oleic acid/behenic acid and beta-amyrin/campesterol ratios

PendingCN112305108ARapid adulterated false differential analysisLow in behenic acidComponent separationBiotechnologyVegetable oil
The invention belongs to the field of food inspection and detection, and discloses a camellia oleosa seed oil adulteration detection method based on oleic acid/behenic acid and beta-amyrin/campesterolratios. Camellia oleosa seed oil and different vegetable oils are saponified, saponified substances are subjected to fatty acid content detection, non-saponified substances are subjected to triterpenoid compound detection, and through two ratios of oleic acid/behenic acid to beta-amyrin/campesterol, the content of fatty acid in the saponified substances is detected. Adulteration qualitative identification analysis can be rapidly carried out on rice oil, sunflower seed oil, corn oil, rapeseed oil, soybean oil, peanut oil and novel high-oleic-acid vegetable oil (high-oleic-acid peanut oil, high-oleic-acid sunflower seed oil and high-oleic-acid rapeseed oil) which are common in the market at present. According to the method, the adulteration in the pure tea oil can be judged through comprehensive judgment of two indexes, namely the ratio of methyl cis-oleate to methyl behenate is smaller than 1200 or the ratio of beta-amyrin to campesterol is smaller than 40, the adulteration characteristic of the pure tea seed oil can be visually presented, and the adulteration of the pure tea seed oil can be qualitatively identified.
Owner:江西省食品检验检测研究院
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