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Method for simultaneously determining phytosterol and squalene in vegetable oil

A technology of vegetable oil and squalene, applied in the direction of measuring devices, instruments, scientific instruments, etc.

Active Publication Date: 2014-04-09
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Because the structures of various phytosterol components are very similar, such as β-sitosterol, stigmasterol, and campesterol, the only difference is that there is a double bond and a CH in the branched chain. 2 group, so it is difficult to separate and measure various components by general chemical methods, especially stigmasterol and campesterol, which are difficult to completely separate the two by high performance liquid phase method in general literature reports

Method used

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  • Method for simultaneously determining phytosterol and squalene in vegetable oil
  • Method for simultaneously determining phytosterol and squalene in vegetable oil
  • Method for simultaneously determining phytosterol and squalene in vegetable oil

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Embodiment 1

[0020] Embodiment 1, the high-performance liquid chromatography method of simultaneous determination of β-sitosterol, stigmasterol, campesterol and squalene in peanut oil

[0021] 1. Sample preparation

[0022] 1. Extraction of peanut oil

[0023] The peanuts were taken from the peanuts planted in Shandong in 2010. After peeling and crushing, the peanuts were shaken and extracted with n-hexane with a solid-to-liquid ratio of 1:6 (g / mL) (at a speed of 155 r / min) for 6 hours; The organic phase is separated from the precipitate by high-speed centrifugation at a speed of 4000r / min; the collected organic phase is recovered by rotary evaporation (temperature 35°C-40°C) in a RE-52AA rotary evaporator produced by "Shanghai Yarong Biochemical Instrument Factory" n-Hexane to obtain a light yellow, clear and translucent peanut crude oil.

[0024] 2. Saponification

[0025] Weigh about 5g of the sample, put it in a 250mL flask, add 30mL of absolute ethanol, 10mL of potassium hydroxide ...

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Abstract

The invention discloses a method for simultaneously determining stigmasterol, campesterol, beta-sitosterol and squalene in vegetable oil. The method comprises the following steps of: 1) saponifying the vegetable oil, and extracting an unsaponifiable matter in a saponification solution by using an organic solvent; and 2) dissolving the unsaponifiable matter in the organic solvent, and determining by using high-performance liquid chromatography, wherein the high-performance liquid chromatography has the chromatographic conditions that a chromatographic column is a C18 column, a mobile phase is a solvent of which the volume ratio of acetonitrile to water is (90:10) to (100:0), an ultraviolet detection wavelength is 205 to 210 nm, a sample size is 10 to 20 mu L, flow speed is 1.0 to 1.5 mL / min and column temperature is 25 to 30 DEG C. By adoption of the method, effective separation of the campesterol, the stigmasterol, the beta-sitosterol and the squalene in the vegetable oil can be simultaneously realized, consumed time for separation is about 40 min, and components in a chromatogram are eluted in a sequence of the campesterol, the stigmasterol, the beta-sitosterol and the squalene.

Description

technical field [0001] The invention relates to a method for simultaneously determining phytosterol and squalene in vegetable oil. Background technique [0002] Phytosterol is a natural active substance that widely exists in plants. There are many kinds of natural phytosterols, mainly including β-sitosterol, stigmasterol, and campesterol. Phytosterols are widely found in various vegetable oils, nuts and plant seeds, and also in other plant foods such as vegetables and fruits. The content of sterols in different plant species is different, such as wheat germ oil, corn germ oil, rice bran oil, The content in flower oil is relatively high, and in the daily intake, phytosterols from cereals, vegetables, and fruits can account for more than 20% of the total intake. The human body cannot synthesize phytosterols freely and can only be ingested from food. Phytosterols have high safety and can be used as new food additives. The chemical structure of phytosterol is similar to that ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 王强张建书刘红芝刘丽
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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