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Method for purifying campesterol from mixed plant sterol

A technology of campesterol and phytosterol, which is applied in the direction of steroids and organic chemistry, can solve the problems of being unable to meet the requirements of physiological activity research and commercial production, and is expensive, and achieves the advantages of simple operation, low cost and increased yield Effect

Inactive Publication Date: 2012-08-15
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on campesterol with the same abundant content, and there are even fewer reports about the separation and refining technology of campesterol. Moreover, the purity of campesterol monomers sold on the market can only reach 60%, and the price is expensive, far from Meet the research and commercial production requirements for its related physiological activities

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Weigh 20 grams of phytosterol (purity 97%, of which campesterol content is 32.9%), add 400 ml of analytical grade acetone to the Erlenmeyer flask with the mass volume ratio of phytosterol and reagent at 1:20, and place at 50 °C Dissolve in a water bath. After all the phytosterols are dissolved, the water bath cools down to 15°C, and white crystals slowly precipitate.

[0029] 2. Put it on the platform and keep it at a constant temperature for 12 hours.

[0030] 3. Heat preservation and vacuum filtration, collect the filter cake and filtrate, the filter cake is air-dried naturally, and the filtrate is evaporated under reduced pressure in a water bath at 40°C to recover sterols and remove organic solvents. The content of campesterol in the sterol recovered from the filter cake and the filtrate was detected by gas chromatography, the gas chromatography column was a phenyl-methyl stationary phase column, nitrogen was used as the carrier gas, and the temperature was progr...

Embodiment 2

[0033] 1. Weigh 10 grams of phytosterol (purity: 97%, campesterol content: 32.9%), add 100 ml of acetone of analytical grade to a Erlenmeyer flask at a mass volume ratio of phytosterol to reagent of 1:10, and place in a 55°C water bath Dissolve, after all the phytosterols are dissolved, the temperature of the water bath is lowered to 10°C, and white crystals slowly precipitate out.

[0034] 2. Put it on the platform and keep it at a constant temperature for 18 hours.

[0035] 3. Heat preservation and vacuum filtration to collect the filter cake and filtrate. The filter cake was air-dried naturally, and the filtrate was rotary-evaporated under reduced pressure in a water bath at 50°C to recover sterols and remove organic solvents. High-performance liquid chromatography was used to detect the content of campesterol in the sterols recovered from the filter cake and filtrate respectively. The chromatographic column type used was an analytical ODS C18 column, the mobile phase was ...

Embodiment 3

[0038] 1. Weigh 5 grams of phytosterol (purity 97%, campesterol content 32.9%), add 75 ml of acetone of analytical grade to a conical flask with a mass volume ratio of phytosterol and reagent of 1:15, and place in a 60°C water bath Dissolve, after all the phytosterols are dissolved, the temperature of the water bath is lowered to 20°C, and white crystals slowly precipitate out.

[0039] 2. Put it on the platform and keep it at a constant temperature for 24 hours.

[0040] 3. Heat preservation and vacuum filtration, collect the filter cake and filtrate, and air-dry the filter cake naturally. The filtrate is evaporated under reduced pressure in a water bath at 45°C to recover sterols and remove organic solvents. Use high-performance liquid chromatography to detect the content of campesterol in the sterols recovered from the filter cake and filtrate respectively. The chromatographic column type used is an analytical C8 column, and the mobile phase is acetonitrile / isopropanol / wate...

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Abstract

The invention discloses a method for purifying campesterol from mixed plant sterol. According to the method disclosed by the invention, different plant sterol components have different solubilities and different degrees of crystallinity in a specific organic solvent, acetone is taken as an organic solvent to perform recrystallization on a plant sterol mixture for multiple times, the contents of all plant sterol monomers are respectively detected by chromatography, the purity of the campesterol in all-level crystals can be determined, and the campesterol with higher purity can be finally extracted. The invention provides the method for purifying the sterol monomers from the mixed plant sterol by only using one organic solvent under large-scale production conditions. The method disclosed by the invention has important theoretical and practical significance in the aspect of extraction of natural and pure products including the plant sterol, can play an important role in fine chemistry industry and analytical chemistry of plants, and has broad application prospects.

Description

technical field [0001] The invention relates to the field of biochemical industry or fine chemical industry, in particular to a method for purifying campesterol from mixed phytosterols. Background technique [0002] Mixed phytosterols are a class of steroidal compounds with similar structures and small differences in physical properties. They are mainly derived from wood pulp tall oil and vegetable oil refining deodorization distillates. The main components are β-sitosterol (β-sitosterol), campesterol ( campesterol), stigmasterol (stigmasterol) and brassicasterol (avenasterol) and so on. [0003] Phytosterols have very important physiological functions, which can effectively reduce low-density cholesterol, reduce the incidence of heart disease, anti-cancer and anti-inflammatory effects. The various physiological functions of phytosterols make them widely used in various fields such as medicine, cosmetics, animal growth agents, food, textile, printing and paper processing in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00
Inventor 高俊兰张欣岳秋林程备久
Owner ANHUI AGRICULTURAL UNIVERSITY
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