Method for purifying campesterol from mixed plant sterol
A technology of campesterol and phytosterol, which is applied in the direction of steroids and organic chemistry, can solve the problems of being unable to meet the requirements of physiological activity research and commercial production, and is expensive, and achieves the advantages of simple operation, low cost and increased yield Effect
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Embodiment 1
[0028] 1. Weigh 20 grams of phytosterol (purity 97%, of which campesterol content is 32.9%), add 400 ml of analytical grade acetone to the Erlenmeyer flask with the mass volume ratio of phytosterol and reagent at 1:20, and place at 50 °C Dissolve in a water bath. After all the phytosterols are dissolved, the water bath cools down to 15°C, and white crystals slowly precipitate.
[0029] 2. Put it on the platform and keep it at a constant temperature for 12 hours.
[0030] 3. Heat preservation and vacuum filtration, collect the filter cake and filtrate, the filter cake is air-dried naturally, and the filtrate is evaporated under reduced pressure in a water bath at 40°C to recover sterols and remove organic solvents. The content of campesterol in the sterol recovered from the filter cake and the filtrate was detected by gas chromatography, the gas chromatography column was a phenyl-methyl stationary phase column, nitrogen was used as the carrier gas, and the temperature was progr...
Embodiment 2
[0033] 1. Weigh 10 grams of phytosterol (purity: 97%, campesterol content: 32.9%), add 100 ml of acetone of analytical grade to a Erlenmeyer flask at a mass volume ratio of phytosterol to reagent of 1:10, and place in a 55°C water bath Dissolve, after all the phytosterols are dissolved, the temperature of the water bath is lowered to 10°C, and white crystals slowly precipitate out.
[0034] 2. Put it on the platform and keep it at a constant temperature for 18 hours.
[0035] 3. Heat preservation and vacuum filtration to collect the filter cake and filtrate. The filter cake was air-dried naturally, and the filtrate was rotary-evaporated under reduced pressure in a water bath at 50°C to recover sterols and remove organic solvents. High-performance liquid chromatography was used to detect the content of campesterol in the sterols recovered from the filter cake and filtrate respectively. The chromatographic column type used was an analytical ODS C18 column, the mobile phase was ...
Embodiment 3
[0038] 1. Weigh 5 grams of phytosterol (purity 97%, campesterol content 32.9%), add 75 ml of acetone of analytical grade to a conical flask with a mass volume ratio of phytosterol and reagent of 1:15, and place in a 60°C water bath Dissolve, after all the phytosterols are dissolved, the temperature of the water bath is lowered to 20°C, and white crystals slowly precipitate out.
[0039] 2. Put it on the platform and keep it at a constant temperature for 24 hours.
[0040] 3. Heat preservation and vacuum filtration, collect the filter cake and filtrate, and air-dry the filter cake naturally. The filtrate is evaporated under reduced pressure in a water bath at 45°C to recover sterols and remove organic solvents. Use high-performance liquid chromatography to detect the content of campesterol in the sterols recovered from the filter cake and filtrate respectively. The chromatographic column type used is an analytical C8 column, and the mobile phase is acetonitrile / isopropanol / wate...
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