The invention provides a composition for inhibiting food digestion and absorption as well as a preparation method and an application of the composition. The composition disclosed by the invention, which is added with such components as a mulberry leaf extract, a white kidney bean extract and the like which can effectively inhibit human body metabolism on starch, fat and glucose, can take a relatively good curative effect and control effect on obesity and the like, and the composition has the advantages of being green and healthy in raw materials and finished product, rapid to take effects, free from side effects and the like. Meanwhile, the invention also provides a preparation method of the composition; according to the preparation method, corresponding compositions can be prepared by mixing various component raw materials; and the preparation method has the advantages of being simple and convenient and the like. Furthermore, the invention also provides an application of the composition; with the addition of the raw material components which can effectively inhibit amylase, lipase and sugar metabolizing enzymes, the composition provided by the invention can effectively inhibit metabolic absorption of the starch, the fat and the glucose to human body, so as to effectively treat the obesity; and in addition, the composition can be used for effectively controlling blood glucose of patients with hyperglycemia.