30 results about "Antibody microarray" patented technology

The present invention provides a monoclonal antibody recognizing modification after translation of p53 in a manner specific to a modification site, an antibody microarray comprising the antibody immobilized on a substrate, etc. Disclosed is a monoclonal antibody which reacts specifically with a peptide consisting of an amino acid sequence of at least 6 consecutive amino acids containing a predetermined amino acid residue of the amino acid sequence represented by SEQ ID NO: 1, wherein the amino acid residue is phosphorylated or acetylated, or with a peptide having one to several arbitrary amino acids added to the above peptide, but does not react with the above peptide which is not phosphorylated or acetylated.

The invention provides a method for detecting one or more biological ligands, where the method generally uses two arrays of biological reagents. The two arrays have two different functionalities: the first array captures the ligands on the array; and the second array delivers detecting reagents to the captured ligands. Use of arrays affords the method high-throughput detection capability; and the use of two different arrays ensures high specificity. The first array has a support structure fixed with a first set of reagents, each at a pre-determined position on the support. The second array has a support structure fixed with a second set of reagents, each at a pre-determined position on the support. The first array contains reagent(s) binding to a ligand, while the second array contains second reagent(s) binding to the same ligand. In preferred embodiments, antibodies are used as reagents, and used in detecting proteins in protein samples.

The invention discloses a fish lymphocystis disease virus (LCDV) immunity detection chip, and the structure of the chip comprises a chip carrier, an agarose gel layer covered on the chip carrier and a plurality of antibody microarrays fixed on the agarose gel layer; wherein, special fences for chips or Super PAP Pen rulings are arranged between the microarrays for separating the microarrays. In the invention, a sandwich method is adopted to detect antigen, pathogenic antibody is fixed on a film support of the chip, the to-be-detected sample solution is prepared by using virus target organ of a sample to be detected, the to-be-detected sample solution and the chip fixed with pathogenic antibody are directly incubated, a specific monoclonal antibody (McAb) probe with a fluorescent mark is added, and then a CCD scanner is used for reading the result. The invention has the advantages of detecting lymphocystis disease virus in various samples or different tissues in the same sample simultaneously, which can be used for rapid, sensitive, and accurate detection on LCDV in marine-culturing fishes and quarantine on LCDV in imported-exported fishes.

A method for constructing an antibody microarray based on a double-antibody sandwich immunoassay, characterized in that the construction steps include: preparation of a carrier; activation of the carrier; spotting and fixing of a capture antibody; and immunoassay based on the microarray. The antibody microarray constructed according to this optimized operation procedure has good reproducibility (intra-group and inter-group variation <10%) and quantitative analysis ability (standard curve correlation between antigen concentration and signal intensity>0.95 ), providing a reliable technical platform for parallelism and multi-factor protein quantitative detection. In addition, the chip is stable after spotting, and the activity can be maintained for at least 2 months, which provides important conditions for the industrialization and scale of the chip.

A genetic polymorphism associated with myocardial infarction is provided. More particularly, provided are a polynucleotide including a single nucleotide polymorphism (SNP) or a haplotype associated with myocardial infarction, a polynucleotide hybridized with the polynucleotide, a polypeptide encoded by one of the polynucleotides, an antibody bound to the polypeptide, a microarray and a kit including one of the polynucleotides, a myocardial infarction diagnosis method, a SNP detecting method and a method of screening pharmaceutical compositions for myocardial infarction.

The invention provides a method for identifying heterophilic antibody interference in an antibody microarray system, which is mainly characterized in that: a heterophilic antibody identification point is increased during manufacturing an antibody microarray chip. The antibody of the identification point consists of analogues of solid phase antibodies of various indexes. The method comprises the following steps of: after samples with statistical significance quantity are detected, establishing judgment criteria of heterophilic antibody positive interference; and judging whether the later clinical samples of the same indexes have the heterophilic antibody positive interference according to the judgment criteria. The method identifies the heterophilic antibodies without increasing the conventional workload and reagents; the method does not need to add a blocking agent into each sample so as to save the consumption of many blocking agents, reduce the cost and reduce unknown interference; and meanwhile, automatic identification and objective judgment can be performed through a biochip reader, and analysis on the clinical symptoms of patients is not needed.

The invention discloses an antibody microarray kit for detecting the residue of aminoglycoside antibiotics in food. The antibody microarray kit comprises a microarray, an antibody, a secondary antibody marked by a Cy3, and an extraction reagent, wherein the antibody consists of a neomycin monoclonal antibody and a gentamycin monoclonal antibody, wherein the neomycin monoclonal antibody is secreted by a hybridoma cell EDC / 5G04 of which the preservation number is CCTCC NO:C201144; the gentamycin monoclonal antibody is secreted by a hybridoma cell EDC / 2D5 of which the preservation number is CCTCC NO:C201145, and the microarray fixes an amikacin coating antigen and a sisomicin coating antigen. The invention further discloses an antibody microarray method for detecting the residue of the aminoglycoside antibiotics in the food. The method can be used for detecting 5 aminoglycoside antibiotics at the same time, so that the method which is quick, is sensitive and is high in flux is provided for detecting the residue of the aminoglycoside antibiotics in the food, and the method has the characteristics of high accuracy, high precision, high efficiency and the like.

The invention discloses an antibody chip kit for simultaneous quantitative detection of multiple receptors. The kit comprises antibody chips, a receptor standards mixture, a biotin labeled detection antibodies mixture and Cy3 fluorochrome labeled streptavidin. A standard tissue slide which is coated by reactive amino groups is chosen as a surface carrier of the antibody chips, and various antigen specific antibodies are adsorbed firmly on the surface of the slide by a non-contact spotting robot and sixteen microarrays are formed. The sixteen microarrays are then separated in non-interfering small areas by the use of a plastic frame with 2*8 holes and a latex closed frame which match each other and are demountable. Each capture antibody has four repeated spottings in each antibody microarray. The antibody chip kit can realize simultaneous quantitative detection of multiple receptors. Dynamic detection range of receptors is wider. Quadruple ELISA repeated data can be obtained from a single sample experiment. The antibody chip kit has advantages of high sensitivity, high throughput, small required use amount of samples, low cost, easy popularization and the like.

The invention discloses an immunity detection chip of prawn white spot syndrome virus (WSSV), comprising a chip carrier and an agarose gel layer which is paved on the chip carrier, wherein, the agarose gel layer is fixed with a plurality of antibody microarrays of 4*4, and chip-dedicated fences or Super PAP Pen scribings are used for separating the microarrays from each other. The invention comprises the following steps: adopting the sandwich method to detect the antigen; fixing the pathogenic polyclonal antibody (PcAb) on the chip slice base; taking the target organ tissue of the individual to be detected to prepare to-be-detected sample liquid; incubating directly the to-be-detected sample liquid with the chip which is fixed with PcAb to capture the antigen and lead the antigen to combine on the chip; adding a specific monoclonal antibody probe marked by fluorescence; and reading results through a CCD chip scanner. The invention has the advantage of detecting white spot syndrome virus (WSSV) in multiple samples simultaneously, and is applicable to the rapid and accurate detection of white spot syndrome virus (WSSV) of prawns / crabs in breeding production and the quarantine inspection of WSSV in import and export prawns / crabs.

The invention provides a micro-array chip based on a sphere-brush double layer nano-structure substrate and a preparation method thereof. The preparation method of the micro-array chip has versatility, and has the advantages of convenient process and less equipment requirement, and is suitable for large batch production; the micro-array chip can realize the analysis detection of interacting between nucleotide and nucleotide, sugar and protein, and protein and protein, and has the advantages of simpleness, less sample consumption and high sensitivity. The experiment result shows that the sugar micro-array chip prepared by the method has the detection limit on biotin-modified castor-oil plant agglutinin-120 and biotin-modified concanavalin agglutinin being 1 ng / mL, and the DNA micro-array chip has the detection limit on target DNA being 0.1 nmol / L; the glycoprotein micro-array chip has the detection limit on the biotin-modified castor-oil plant agglutinin-120 being 0.3 ng / ml, and the antibody micro-array chip has the detection limit on the CY5-modified rabbit-anti-human antibody being 10 pg / mL.

The invention provides a method for detecting one or more biological ligands, where the method generally uses two arrays of biological reagents. The two arrays have two different functionalities: the first array captures the ligands on the array; and the second array delivers detecting reagents to the captured ligands. Use of arrays affords the method high-throughput detection capability; and the use of two different arrays ensures high specificity. The first array has a support structure fixed with a first set of reagents, each at a pre-determined position on the support. The second array has a support structure fixed with a second set of reagents, each at a pre-determined position on the support. The first array contains reagent(s) binding to a ligand, while the second array contains second reagent(s) binding to the same ligand. In preferred embodiments, antibodies are used as reagents, and used in detecting proteins in protein samples.

The invention relates to a method and a device for detecting free epithelial cell organ sources in blood. The method includes the following steps: capturing epithelial cells from a blood sample; cracking the epithelial cells to release protein therein; specifically recognizing at least four protein markers with organ specificity; acquiring the organ sources of the epithelial cells by detecting expression quantity of the protein markers. The device comprises a microgroove chip and an antibody loading glass piece, 100-10000 microgrooves are formed in the microgroove chip and used for containing the cells, the antibody loading glass piece is used for loading an antibody micro-array which comprises at least four mutually-independent antibody strips, and the microgrooves and the antibody micro-array are arranged in a matched manner to enable at least one part of each antibody strip to be contained at corresponding positions of the microgrooves. The problem of detecting the epithelial cell organ sources in blood is solved by quickly and effectively detecting multiple proteins in single cells.

The invention discloses an improved antibody chip kit capable of quantitatively detecting multiple cell factors synchronously. The kit includes a standard tissue slide which is coated with activated amino groups and is used as a surface carrier. Various antigen-specificity antibodies are spotted on a surface of the slide to form a microarray through a non-contacting sample spotting instrument. The slide is divided into sixteen small zones, which are not interfered with each other, through a detachable 2*8-hole plastic frame, wherein each small zone contains an antibody microarray. Each captured antibody is repeated by four times in each antibody microarray. The slide and the frame are tightly clamped with each other through plastic clamping plates which are arranged at the two sides to form an antibody chip. Experimental reagents of a multiple sandwich ELISA reaction are completed on the surface of the slide. By means of the antibody chip kit, multiple cell factors can be quantitatively detected synchronously. A sensitivity of the kit is similar to that of single ELISA but a dynamic detection range of the cell factors is wider. Repeated data, which is as four times as that of ELISA, can be obtained from a single-sample experiment. The kit is high in sensitivity, is high in flux, is little in sample usage amount, is low in cost and is easy to popularize.