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Method for identifying heterophilic antibody interference in antibody microarray system and antibody microarray chip using same for detecting target antigen

A heterophilic antibody and microarray technology, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the interference of heterophilic antibodies, cannot effectively identify heterophilic antibodies, and cannot completely eliminate the influence of heterophilic antibodies, etc. problem, to achieve the effect of large amount of information, reducing unknown interference, and low cost

Active Publication Date: 2014-03-12
上海昌润生物科技有限公司
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Problems solved by technology

[0006] An object of the present invention is to overcome the defects that the above-mentioned existing methods cannot completely eliminate the influence of heterophilic antibodies or cannot effectively identify heterophilic antibodies, and provide a method for identifying the interference of heterophilic antibodies in an antibody microarray system

Method used

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  • Method for identifying heterophilic antibody interference in antibody microarray system and antibody microarray chip using same for detecting target antigen
  • Method for identifying heterophilic antibody interference in antibody microarray system and antibody microarray chip using same for detecting target antigen
  • Method for identifying heterophilic antibody interference in antibody microarray system and antibody microarray chip using same for detecting target antigen

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Embodiment

[0066] Antibody microarray detection system for detecting 11 tumor markers

[0067] 1. Main raw materials:

[0068] The sources of antibodies and antigens for 11 tumor markers are shown in Table 1:

[0069] Table 1 Sources of antibodies and antigens for 11 tumor markers

[0070] Tumor markers product code manufacturers CA19-9 primary antibody M8073022 Meridian., US CA153 primary antibody M37901M Meridian., US CA242 primary antibody 101-01 Fujirebio Diagnostics CA125 primary antibody M86306M Meridian., US SCC primary antibody SCC113 Fujirebio Diagnostics CK19 primary antibody 120-01 Fujirebio Diagnostics NSE primary antibody 405-01 Fujirebio Diagnostics CEA primary antibody M0911042 Fitzgerald CO., US AFP primary antibody M19301 Fitzgerald CO., US B-HCG primary antibody M15294 Fitzgerald CO., US PSA primary antibody M86506M Meridian., US CA19-9 Secondary ...

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Abstract

The invention provides a method for identifying heterophilic antibody interference in an antibody microarray system, which is mainly characterized in that: a heterophilic antibody identification point is increased during manufacturing an antibody microarray chip. The antibody of the identification point consists of analogues of solid phase antibodies of various indexes. The method comprises the following steps of: after samples with statistical significance quantity are detected, establishing judgment criteria of heterophilic antibody positive interference; and judging whether the later clinical samples of the same indexes have the heterophilic antibody positive interference according to the judgment criteria. The method identifies the heterophilic antibodies without increasing the conventional workload and reagents; the method does not need to add a blocking agent into each sample so as to save the consumption of many blocking agents, reduce the cost and reduce unknown interference; and meanwhile, automatic identification and objective judgment can be performed through a biochip reader, and analysis on the clinical symptoms of patients is not needed.

Description

technical field [0001] The invention relates to a method for identifying heterophilic antibody interference in an antibody microarray system and an antibody microarray chip using the same to detect target antigens. Background technique [0002] Analysis of multiple proteins in patient serum to guide diagnosis, treatment, and prognosis has promoted the development of parallel protein analysis methods (Expert Rev Mol Diag 2007; 7:87-98.). This method of parallel analysis of multiple proteins is called protein chip, protein microarray chip, etc. The current multi-index analysis method is mainly based on the traditional immunological principle, immobilizing the antigen or antibody on the solid phase surface in the form of an array, specifically binding to the corresponding target substance in the serum, and combining with the labeled antibody, The markers can be photographed or scanned by the protein chip reader, and the computer software analyzes the scanned or photographed re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577
Inventor 杨小丽季海鹏林晓琳
Owner 上海昌润生物科技有限公司
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