694results about "Detection of post translational modifications" patented technology

The invention provides methods and compositions for simultaneously detecting the activation state of a plurality of proteins in single cells using flow cytometry. The invention further provides methods and compositions of screening for bioactive agents capable of coordinately modulating the activity of a plurality of proteins in single cells. The methods and compositions can be used to determine the protein activation profile of a cell for predicting or diagnosing a disease state, and for monitoring treatment of a disease state.

The present invention provides methods for rapid deglycosylation of glycoproteins.

This invention provides methods for determining or predicting response to HER2-directed therapy in an individual.

Compositions and methods of treatment for abnormal bone density are disclosed based upon the finding that sclerostin must be bound to glypican in order to inhibit bone deposition. Methods for identifying agents that inhibit the glypican-sclerostin interaction are disclosed for treatment of bone deposition disorders. Diagnostic methods are also disclosed.

The invention relates to determining the presence of an EZH2 gene mutation in a sample from a subject and inhibition of wild-type and certain mutant forms of human histone methyltransferase EZH2, the catalytic subunit of the PRC2 complex which catalyzes the mono-through tri-methylation of lysine 27 on histone H3 (H3-K27). In one embodiment the inhibition is selective for the mutant form of the EZH2, such that trimethylation of H3-K27, which is associated with certain cancers, is inhibited. The methods can be used to treat cancers including follicular lymphoma and diffuse large B-cell lymphoma (DLBCL). Also provided are methods for identifying small molecule selective inhibitors of the mutant forms of EZH2 and also methods for determining responsiveness to an EZH2 inhibitor in a subject.

The invention relates to the technical field of comparative proteomics, and in particular to a detection and quantification method for post-translational modification proteomics. The method is characterized in that equiponderous tandem mass tag labeling is carried out on a to-be-detected protein sample and an internal standard substance, and tandem mass spectral analysis is carried out on a labeled mixture, wherein the internal standard substance is a mixture which is rich in to-be-detected post-translational modification peptide fragments. According to the method, a signal containing the to-be-detected post-translational modification peptide fragments can be amplified, and therefore under the circumstances that the sensitivity of the mass spectrum is not changed and the post-translationalmodification peptide fragments do not need to be enriched, the probability that the post-translational modification peptide fragments are subjected to mass spectrometry detection and MS / MS analysis can be improved.

The invention relates to a chemiluminescent protein chip, kit and detection method for detection of the fucose index of seroglycoid, belonging to the field of protein detection technology. The chemiluminescent protein chip is characterized in that a substrate slide of the protein chip at least comprises a detection subdomain; one detection subdomain is used for detection of one serum sample; each detection subdomain is provided with two detection spot areas and one row of contrast spot areas; one of the detection spot areas has detection spots formed by specific antibodies used for immobilizing alpha fetoprotein, and the other of the detection spot areas has detection spots formed by immobilization of Lens culinaris agglutinin; the contrast spot areas have contrast spots formed by immobilization of bovine serum albumin; and concentrations of substances on the detection spots in a same detection spot area are identical. The protein chip, kit and method provided by the invention can realize accurate and high flux detection of the fucose index of seroglycoid and have the advantages of high sensitivity, saving of time, convenience, economic performance and the like in clinical application.