The invention discloses a test strip for detecting an anti-cyclic citrullinated peptide antibody by colloidal gold chromatography and a preparation method thereof. The test strip comprises a baseplate (7) and a nitrocellulose membrane (3), a conjugate pad (2), a sample pad (1) and a water-absorbing pad (6), which are sequentially stuck on the baseplate (7) by mutual lap joint. A detection region (4) and a quality control region (5) are arranged on the nitrocellulose membrane, the detection region is used for coating a cyclic citrullinated peptide antigen, the quality control region is used for coating the antibody which is used as a quality control material, and the conjugate pad is used for coating two different colloidal gold labeled antibodies. The test strip introduces the branch cyclic citrullinated peptide and carries out process improvement on pretreatment of the sample pad and the conjugate pad, and the invention provides the anti-cyclic citrullinated peptide antibody test strip with high sensitivity and strong specificity, which can quickly screen positive samples and further play an important role for early auxiliary diagnosis of RA and disease monitoring.