185results about How to "Enable high-throughput detection" patented technology

The invention relates to inspection and quarantine systems for biomedicine, agriculture, food hygiene and the like and particularly relates to an absolute quantification type digital nucleic acid analytic system based on an efficient liquid drop microreactor. The absolute quantification type digital nucleic acid analytic system is mainly used for detecting the molecular structure of nucleic acid so as to analyze attributes of organisms and belongs to the fields of biological and medical detection. The absolute quantification type digital nucleic acid analytic system comprises a confocal fluorescence image collecting system, a micro-fluidic chip, a temperature circulating heating system, an electric loading platform, an automatic sample feeding device, a controller and a computer. The formation and PCR amplification of micro-droplets are finished by virtue of the same micro-fluidic chip, namely the micro-fluidic chip has a droplet formation function and a PCR amplification (microreactor) function. According to the absolute quantification type digital nucleic acid analytic system, the complexity of the absolute quantification type nucleic acid analysis process is reduced, the nucleic acid analysis process is finished in a one-button manner, and culture and bacteria-proliferating processes are omitted, so that the rapid detection is realized, the sensitivity of the absolute quantification type nucleic acid detection is greatly improved, meanwhile, the sample consumption of the absolute quantification type nucleic acid analysis is substantially reduced, and the high throughput detection is realized.

The invention, which belongs to the biological field, particularly relates to a method for identifying and evaluating the toxigenic capability of a toxigenic strain of aflatoxin. A ratio of the aflatoxin yield to the Nor-1 gene transcription amount is determined and the high relative stability is realized. An aflatoxin strain toxigenic capability identification model is established and thus a regression equation between the aspergillus flavus toxigenic capability and the AFT / Nor-1 ratio is obtained. With determination of the AFT / Nor-1 ratio, the toxigenic capability of the aspergillus flavus strain is identified and evaluated rapidly and accurately, so that the method has the important significance in aflatoxin pollution early warning and prevention controlling. Besides, a synchronous detection RT-PCR method for the aflatoxin yield and the nor-1 gene transcription amount is also established; and the AFT / Nor-1 ratio obtained based on the synchronous detection RT-PCR method is reliable and accurate and can be used as the identification index for determining the toxigenic capability of the aspergillus flavus strain.

The invention relates to a high-flux micro-fluidic chip nucleic acid amplification analysis detection system which is characterized by comprising a revolution-rotation composite movement platform, micro-fluidic chips, a multi-axis movement control system, a multi-way PID temperature control system, an optical detection system and a data acquisition processing and display system, wherein more than two revolution movement positioning shafts are arranged at circumference graduation intervals on the revolution-rotation composite movement platform; each micro-fluidic chip is fixedly arranged on the revolution movement positioning shaft of the revolution-rotation composite movement platform; the multi-axis movement control system is used for controlling movement of the revolution-rotation composite movement platform; the multi-way PID temperature control system is used for adjusting and controlling the temperature of each micro-fluidic chip; the optical detection system is used for detecting the high sensitivity of a trace sample placed inside each micro-fluidic chip in real time, and used for transmitting the detection result to the data acquisition processing and display system in real time. By adopting the high-flux micro-fluidic chip nucleic acid amplification analysis detection system, the high-flux nucleic acid amplification detection application requirement of dozens to two hundred of samples every day can be met.

The invention provides a primer, a probe, a reagent kit and a method for detecting mutation of BRAF gene V600E on the basis of real-time fluorescent quantitative PCR (polymerase chain reaction) technical platform. The primer, the probe, the reagent kit and the method are quick and low in cost and have clinic detection popularization value.

The invention discloses a porcine circovirus type 2 antigen capture ELISA kit. The kit internally comprises an enzyme labeled monoclonal antibody which is secreted by a hybridoma cell strain with the preservation number of CGMCC NO.10205. The invention also discloses a capture ELISA method for rapidly detecting the porcine circovirus type 2 and established by utilizing the monoclonal antibody. A porcine circovirus type 2 polyclonal antibody and a porcine circovirus type 2 cap protein monoclonal antibody respectively act as a capture antibody and a detecting antibody. The method can be used for detecting multiple gene type PCV2 viruses; the detection sensibility is 400 TICD50 / ml; the porcine circovirus type 2 has no crossed reaction with other swine viruses, and the toxic value determination method coincidence rate is 88%. The result shows that the method has the advantages of operation simplicity, good specificity, high sensitiveness, short time consumption and the like, can be used for estimating the toxic value of viruses, and can conveniently and rapidly control the quality of PCV2 inactivated vaccine semi-finished products.

The invention relates to a method for measuring antigen protein in serum by chemiluminescence protein chips and a kit, which belongs to the medical detection technology. The method is characterized in that a chemiluminescence technology, a standard curve and a protein chip technology are combined for performing quantitative determination of certain antigen protein substance in serum, the high specialty and sensitivity of the chemiluminescence technology and the standard curve are employed for realizing the quantitative determination; the protein chip technology can realize simultaneous detection and analysis of several samples, so that the detection time consumption of a whole set ofe sample is greatly reduced, and the method has the characteristics of reliability, accuracy and low cost; and the method is suitable for general analysis of common antigen protein level in serum and quantitative analysis of great disease antigen marks, and the method is an medical auxiliary detection method having great meaning. The invention also provides a matched kit based on the method.

The invention discloses an EGFR (epidermal growth factor receptor) gene mutation site detection kit which detects EGFR gene mutation sites by combining the ARMS (amplification refractory mutation system) specific mutant enrichment technology with the Taqman-MGB (minor groove binder) probe specific detection technology. The kit comprises ARMS primer pairs and corresponding Taqman-MGB probes, and the ARMS primer pairs and the corresponding Taqman-MGB probes are used for detecting the EGFR gene mutation sites. The EGFR gene mutation site detection kit is high in sensitivity, capable of detecting multiple samples simultaneously and low in cost, provides guidance for pharmacy of clinicians, realizes individualized treatment of patients suffering from tumors and is capable of reducing treatment risk and burden of the patients and wide in application prospect.

The invention discloses a matrix-assisted excitation spectrum detection system adopting plasma surface sample injection. The matrix-assisted excitation spectrum detection system adopting plasma surface sample injection comprises a carrier gas system, a microwave source system, a sample injection system, a microwave induced plasma excitation source, a detection system for carrying out collection, transmission, beam splitting and detection on the detection light emitted by the microwave induced plasma excitation source, and a data processing system for carrying out analysis processing on the output signal of the detection signal, wherein the carrier gas system is connected with the gas discharge tube inlet of the microwave induced plasma excitation source, the signal input end of the data processing system is connected with the signal output end of the detection system; the microwave induced plasma excitation source is connected with the microwave source system through a microwave transmission line. According to the matrix-assisted excitation spectrum detection system adopting plasma surface sample injection, disclosed by the invention, elemental analysis is carried out in a manner of surface sample injection; the system is miniaturized in composition, good in production stability, high in sensitivity, simple in device, simple and convenient to operate, low in sample consumption, high in analysis speed, low in energy consumption, and low in gas consumption.

The invention provides a primer, probe, fluorescent PCR kit and method for detecting nucleotide polymorphisms of two sites of c.665 and c.1286 in an MTHFR (Methylene Tetrahydrofolate Reductase) gene. The nucleotide types of the two polymorphic sites of c.665 and c.1286 in the MTHFR gene are detected on a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) technology platform according to the principle of 'allelic specific PCR'.

The invention relates to a composition and method for detecting drug resistance of staphylococcus aureus, in particular to a method for using DHPLC for carrying out detection on a multiplex PCR amplification product after multiplex PCR amplification. The determination of the drug resistance of the staphylococcus aureus can be easy, convenient, rapid, and high in sensitivity and specificity through the composition and method for detecting the drug resistance of the staphylococcus aureus.

The invention provides primers, a probe, a fluorescent PCR kit and a detection method for detecting a human HLA-B*1301 allele. According to the theory of 'allele specific PCR', a human leucocyte antigen HLA-B*1301 allele can be detected on a real-time fluorescent quantitative PCR technical platform.

The invention relates to a chemiluminescent protein chip, kit and detection method for detection of the fucose index of seroglycoid, belonging to the field of protein detection technology. The chemiluminescent protein chip is characterized in that a substrate slide of the protein chip at least comprises a detection subdomain; one detection subdomain is used for detection of one serum sample; each detection subdomain is provided with two detection spot areas and one row of contrast spot areas; one of the detection spot areas has detection spots formed by specific antibodies used for immobilizing alpha fetoprotein, and the other of the detection spot areas has detection spots formed by immobilization of Lens culinaris agglutinin; the contrast spot areas have contrast spots formed by immobilization of bovine serum albumin; and concentrations of substances on the detection spots in a same detection spot area are identical. The protein chip, kit and method provided by the invention can realize accurate and high flux detection of the fucose index of seroglycoid and have the advantages of high sensitivity, saving of time, convenience, economic performance and the like in clinical application.

The invention relates to a kit for building a library for detecting hereditary deafness genes and its application. The kit includes an amplification primer set for amplifying hereditary deafness genes; Primers designed for the polymorphism of the gene and the mutation site of the 12s rRNA gene. The present invention adopts multiplex PCR technology, which can detect more than 20 mutation sites of deafness genes in a single reaction; in conjunction with the "double label" system, the DNA amplification product of each sample has two sets of independent label sequences, so The amplification products of all samples can be mixed and sequenced simultaneously to achieve high-throughput detection, thereby greatly reducing the detection cost of a single sample.

The invention provides a complete premixed multiple primer-specific PCR detection kit and method for detecting nucleotide polymorphisms at three sites of c.677 and c.1298 of the folic acid metabolic pathway and c.1298 of the MTRR gene. "Allele-specific PCR" principle, design multiple primer-specific PCR, realize two-tube reaction on the real-time fluorescent quantitative PCR technology platform, and simultaneously detect three folic acid metabolic pathway MTHFR genes c.677 and c.1298 and MTRR gene c.66 The nucleotide type of the site. In addition, modified Taq DNA polymerase and disaccharide PCR protective agent are added to the reaction system to increase the stability of the reaction system and realize the long-term stability of the pre-mixed detection system.

The invention relates to a high throughput test method for a tomato bacterial disease, and more particularly relates to a high throughput test method which can utilize a locking probe to carry out reverse dot blot hybridization on tomato bacterial wilt original bacteria, tomato ulcer pathogenic bacteria and a tomato spot disease, and belongs to the crop preventing and curing disease and exit and entry plant quarantine range. The high throughput test method provided by the invention is based on a rolling circle amplification technology of the locking probe, a pair of universal primers are utilized for amplifying multiple pathogens, then, the high throughput test method and a reverse dot blot are combined together, a result is judged through a chromogenic reaction, the testing time of the traditional tomato bacterial disease is shortened, the specificity and the sensitivity of the detection are guaranteed, and the high throughput test is realized. According to the method, the testing cycle of the traditional pathogenic bacteria can be shortened, the cargo clearance can be increased, the intercept and capture relevance ratio of epidemic situations of imported bulk farm-product is improved, the pest invasion capacity is improved, the cargo clearance of cargoes imported and exported at ports in China is accelerated, and the enterprise cost is reduced and the high throughput test method has the important meanings.

A flow cytometry detection device and method are disclosed. The device includes a sample quantification module, an incubation reaction module, a flow cytometry detection module and a cleaning and waste liquid collecting module. The sample quantification module, the incubation reaction module, the flow cytometry detection module and the cleaning and waste liquid collecting module share a sample injection pump used for suctioning, injecting and pushing a sample, a sheath fluid injection pump used for injection of a sheath fluid and a diluting fluid, and agent injection pumps used for injection of agents. According to the technical scheme, conventional sample pushing and sample suctioning and injecting pumps are combined, and sheath fluid and diluting fluid injection pumps are combined, and therefore the number of injection pumps is effectively reduced, functions of sample suctioning, dilution, sample preparation and sample flow formation are achieved, apparatus redundancy is reduced, thecost is reduced, increased detection periods caused by a serial working mode of apparatuses is reduced to the utmost, and a high throughput test is achieved.

The invention relates to a protein chip and a kit for detecting abnormal DCP (Des--Carboxy-Prothrombin) in serum and a preparation method thereof, and belongs to the technology of protein detection. The protein chip for detecting the abnormal DCP in the serum is characterized in that substrate slide glass of the protein chip at least comprises a detecting subarea, and one detecting subarea detectsone serum sample; a detecting spot area and a control spot area are formed in the detecting subarea; the detecting spot area has detecting spots formed by fixing a DCP specific antibody; the controlspot area has control spots formed by fixing bovine serum albumin; the concentrations of the substances on all detecting spots in the same detecting spot area are the same; the total amount of the DCPspecific antibody fixed on each of the detecting spot is 3nl; each detecting spot is formed by spraying 10 times, and 300pl is spotted each time. The protein chip and the kit disclosed by the invention can accurately detect the abnormal DCP; in clinical use, the protein chip and the kit have the advantages of high sensitivity, time conservation, convenience and quickness, economy and the like.

The invention discloses a bridge-PCR-based high-flux chip detection method for DNA hydroxymethylation. . The detection method comprises: firstly fixing different forward primers for amplyfying DNA hydroxymethylation sites to a chip or a microballon; secondly using beta-glucanotransferase to glycosylating all 5hmC on DNA, then using MspI enzyme for enzyme digestion, wherein hydroxymethylated CCGG cannot be cleaved and non-hydroxymethylated CCGG can be cleaved; thirdly hybridizing genome DNA subjected to enzyme digestion with the chip, and performing bridge PCR, wherein the hydroxymethylated CCGG sites can have amplification because the hydroxymethylated CCGG cannot be cleaved, and conversely the non-hydroxymethylated CCGG sites cannot have amplification because the non-hydroxymethylated CCGG can be cleaved; and finally, according to presence or absence of fluorescence at different matrix points of the chip, determining whether CCGC at different positions of the genome DNA is subjected to hydroxymethylization. The beneficial effects comprises: parallelism detection on different DNA hydroxymethylization sites is realized, and the detection results have the characteristics of high flux and high sensitivity.

The invention provides a liquid crystal biosensing method for detecting UO2<2+>. The liquid crystal biosensing method comprises the steps of firstly modifying a TEA / DMOAP mixed self-assembling film on the surface of a sensing substrate, fixing a capture probe to the surface of a lower slide substrate modified by virtue of a chemical sensitive film, then dropwise adding a mixed solution obtained through reaction of UO2<2+> and a DNA enzyme double chain compound, assembling an upper slide modified with a DMOAP sensitive film and a lower slide modified with a TEA / DMOAP mixed sensitive film and linked with DNA face to face so as to form a liquid crystal box, and applying the liquid crystal box to a polarizing microscope so as to observe optical signal response. According to the liquid crystal biosensing method for detectinf UO2<2+>, a simple and visual liquid crystal biosensing method is developed, and the detection of UO2<2+> is realized. The method has the advantages that the operation is simple, the specificity is good, the marking is not required, and the like.

The invention relates to a hepatitis B virus (HBV) nucleic acid quantitative detection method and a kit. The method includes: employing a magnetic bead technique to extract HBV and internal reference nucleic acid from a sample to serve as a template, and finishing HBV nucleic acid quantitative detection based on a fluorescent PCR technology. The introduced internal reference can be used for monitoring the loss and misoperation of the nucleic acid template or existent PCR inhibitors during operation, thus avoiding a false-negative result. An adopted dUTP-UNG enzyme system can reduce cross contamination of amplification products and prevents a false-positive result. The kit includes a nucleic acid extraction reagent, an amplification reagent, quantitative calibration substances, reference substances and the internal reference. The method and the kit solve the shortcomings of poor detection accuracy and low sensitivity in the prior art, and are suitable for application in clinical HBV nucleic acid quantitative detection.

The invention provides a detection kit. The kit adopts UGT1A1*60(-3279T / G), UGT1A1*93(-3156G / A), UGT1A1*28(-536 / 7) and UGT1A1*6(211G / A) four loci as the detection objects, and takes molecular beacon probe fluorescent quantitative PCR as the basis to carry out genotyping detection. The kit provided by the invention can detect four loci at one time, greatly saves cost, and provides a reliable method for clinical diagnosis and scientific research of relevant fields.

The invention relates to the field of molecular detection, and in particular relates to a primer pair, a kit and a detection method for judging abamectin resistance of tetranychus urticae. The primer pair comprises (1) upstream primers for detecting resistant and susceptible populations, which are RF:5'-AAGCTGTTGACATTTGGACGA-3' and SF:5'-AAGCTGTTGACATTTGGACGG-3', and (2) a common downstream primer which is paired with the upstream primers for use. According to the primer pair, the kit and the detection method, the resistance or susceptibility of tetranychus urticae to abamectin is judged by detecting GluCl (Glutamate-gated chloride channel) gene mutation in tetranychus urticae. The primer pair, the kit and the detection method have the advantages of high speed, effectiveness, high sensitivity, capability of realizing high-flux detection of a large number of samples and the like, and have the effects of monitoring the resistance gene frequency and resistance development trend of the field population of tetranychus urticae to abamectin, timely adjusting a prevention and control strategy for tetranychus urticae, guiding rational drug use and delaying the resistance development.

The invention relates to a device, a system and a method for real-time fluorescence quantitative nucleic acid amplification detection. The device comprises a micro-fluidic unit, a sample injection unit, a temperature control unit and a driving unit; the micro-fluidic unit is provided with one or more parallel annular flow channels; the annular flow channels are provided with a liquid inlet and a liquid outlet; the sample injection unit is arranged at the liquid inlet of the annular flow channels and is used for injecting samples into the annular flow channels; the temperature control unit is matched with the micro-fluidic unit; the driving unit is matched with the micro-fluidic unit and is used for driving a micro-fluid in the annular flow channels to move; at least one section of the annular flow channels is made of a transparent material, so that fluorescence detection is facilitated; and different flow sections of the annular flow channel have different preset temperatures through configuration of the temperature control unit, and through moving in the flow sections with different temperatures in the annular flow channels, the micro-fluid realizes temperature change operation. The invention simplifies nucleic acid amplification and detection operation, is flexible to control, reduces detection time and material cost, and improves detection efficiency.

The invention provides a primer, a probe, a fluorescence PCR kit and a method for detecting nucleotide polymorphism at *6-type and *28-type loci of the UGT1A1 gene. According to the allele specific PCR principle, the nucleotide type at *6-type and *28-type polymorphism loci of the methylenetetrahydrofolate reductase UGT1A1 gene is detected on a real-time fluorescence quantitative PCR technical platform.

The invention relates to an optical fiber sensing micro-fluidic chip nucleic acid amplification in-situ real-time detection system and method. The system comprises a white light source, a detecting light path, a micro-fluidic chip and a spectrum collecting, processing and display module which are sequentially connected, wherein the detecting light path is used for transmitting white light generated by the white light source to the micro-fluidic chip and transmitting optical signals passing the micro-fluidic chip to the spectrum collecting, processing and display module; the micro-fluidic chipis used for performing biochemical reaction; the spectrum collecting, processing and display module is used for collecting the optical signals passing the micro-fluidic chip, analyzing the signals andgenerating a visual biochemical reaction real-time dynamic-change signal curve. The system has the advantages that a method using white light to interfere hyper-spectrum is used to detect nucleic acid amplification information, both a fluorescently-labeled to-be-detected object and a to-be-detected object which is not fluorescently labeled can be detected, and the problems that a fluorescent labeling method affects biological reaction activity, and fluorescence decay cancellation is instable are solved.

Disclosed is a transcription factor protein detection method through exonuclease III digestion FRET-dsDNA micro array chips, wherein the expression and activation levels of the transcription factor are analyzed through the following steps: (1) preparing FRET-dsDNA micro array chips, (2) reacting the transcription factor with FRET-dsDNA micro array chips, (2) reacting the exonuclease III with FRET-dsDNA micro array chips, (1) proceeding detection and analysis to the FRET-dsDNA micro array chips.

The invention discloses a neural photoelectrode and a preparation method thereof, and belongs to the field of preparation of neural photoelectrodes. The neural photoelectrode comprises a sapphire light array device, a wire electrode and ultraviolet glue. The wire electrode is fixed to the surface of the sapphire light array device through the ultraviolet glue. The sapphire light array is attachedto the wire electrode to isolate a stimulation channel and a recording channel in a spatial range, and effectively reduce electrical stimulation noise. The neural photoelectrode is convenient to assemble, and the position and the quantity of the wire electrode can be determined according to the requirements. The light array mode adopted by a light source facilitates stimulation and recording of multiple brain regions, and achieves high-throughput detection of neural activity.

The invention relates to a trapping method, an extraction method and a determination method for carbonyl compounds and tobacco-specific nitrosamines in mainstream cigarette smoke and belongs to the technical field of physical and chemical inspection of mainstream cigarette smoke. The trapping method for the carbonyl compounds and tobacco-specific nitrosamines in the mainstream cigarette smoke disclosed by the invention comprises the following steps: preparing a pretreatment solution from 0.20mol / L hydrochloric acid and absolute ethyl alcohol according to a volume ratio of 1:1; balancing the pretreatment solution on a standard filter for 2 hours so as to obtain a pretreated filter; and taking the standard filter as an auxiliary filter, enabling the mainstream cigarette smoke to sequentially pass through the pretreated filter and the auxiliary filter, and trapping the carbonyl compounds and tobacco-specific nitrosamines in the mainstream cigarette smoke. According to the trapping method disclosed by the invention, twelve harmful components in the carbonyl compounds and tobacco-specific nitrosamines of the mainstream cigarette smoke can be simultaneously trapped due to one-time smoking, so that the working efficiency is doubled. Moreover, the influence that large dead volume is caused during solution trapping can be eliminated.

The invention provides a primer, a probe, a fluorescence PCR (Polymerase Chain Reaction) kit and a method for detecting HLA (Human Leucocyte Antigen)-B*5801 alleles. Human leucocyte antigen (HLA)-B*5801 alleles are detected on a real-time fluorescent quantitative PCR technical platform according to the principle of 'allele specific PCR'.

The invention relates to a method for quantitatively detecting a SiO2-FOtargeted nano-drug carrier on the basis of direct competitionfluorescence immunoassay. SiO2-FO nano particles are subjected to quantitative detection and analysis through combination with specificity of antigen and antibody reactions and sensitivity of fluorescence. The method comprises steps as follows: a SiO2-FOenvelope antigen and animmunogen are prepared, the immunogen is injected into a body of an animal, a high-specificity antibody is obtained and marked with FITC(fluorescein isothiocyanate), a fluorescent antibody is prepared, and content of SiO2-FO is accurately measured through established direct competitionfluorescence immunoassay. The detection method is simple to operate, sensitivity and specificity are high, and high-throughput detection can be realized.