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A flow cytometry detection device and method

A flow cytometry and detection device technology, applied in the field of biomedical detection, can solve the problems of complex structure, high cost, and high failure rate of flow cytometry detection devices, and achieve the effects of reducing the length of detection cycle, reducing quantity and reducing cost

Inactive Publication Date: 2018-06-15
SHANDONG EXCELLENCE BIOTECHNOLOGY CO LTD
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Problems solved by technology

[0008] The present invention aims to provide a flow cytometry detection device and method to solve the technical problems of complex structure, high failure rate or high cost of the flow cytometry detection device in the prior art

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  • A flow cytometry detection device and method

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Embodiment Construction

[0029] It should be noted that, in the case of no conflict, the embodiments in the present application and the features in the embodiments can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and examples.

[0030] According to a typical embodiment of the present invention, a flow cytometry detection device is provided. The flow cytometric detection device includes a sample quantitative module, an incubation reaction module, a flow cytometric detection module, and a cleaning and waste liquid collection module. The sample quantitative module, the incubation reaction module, the flow cytometric detection module, and the cleaning and waste liquid collection module share: The sample injection pump is used for drawing and injecting and pushing the sample; the sheath liquid injection pump is used for the injection of the sheath liquid and diluent; and the reagent injection pump is used for the injection ...

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Abstract

A flow cytometry detection device and method are disclosed. The device includes a sample quantification module, an incubation reaction module, a flow cytometry detection module and a cleaning and waste liquid collecting module. The sample quantification module, the incubation reaction module, the flow cytometry detection module and the cleaning and waste liquid collecting module share a sample injection pump used for suctioning, injecting and pushing a sample, a sheath fluid injection pump used for injection of a sheath fluid and a diluting fluid, and agent injection pumps used for injection of agents. According to the technical scheme, conventional sample pushing and sample suctioning and injecting pumps are combined, and sheath fluid and diluting fluid injection pumps are combined, and therefore the number of injection pumps is effectively reduced, functions of sample suctioning, dilution, sample preparation and sample flow formation are achieved, apparatus redundancy is reduced, thecost is reduced, increased detection periods caused by a serial working mode of apparatuses is reduced to the utmost, and a high throughput test is achieved.

Description

technical field [0001] The invention relates to the field of biomedical detection, in particular to a flow cytometry detection device and method. Background technique [0002] In the field of blood cell / cell analysis, flow cytometry is generally used, that is, the sample cells are constrained into a single cell flow, and after passing through the sensor, pulses of different amplitudes and numbers are generated by laser irradiation detection or changes in the electric field impedance between electrodes, so that the cells are analyzed. Analysis of information such as count and size. [0003] The core principle of flow focusing is the principle of laminar flow in the pipeline. The fluid is composed of two parts: sample flow and sheath flow. In the pipeline, the sheath flow wraps the sample flow and passes through the sensor. Conforms to the velocity gradient distribution of the measured flow. [0004] The mainstream models on the market have little difference in the core tech...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14
CPCG01N15/14G01N15/1404
Inventor 马永波张国联
Owner SHANDONG EXCELLENCE BIOTECHNOLOGY CO LTD
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