Primer, probe, fluorescent PCR kit and method for detecting polymorphism of human MTHFR (Methylene Tetrahydrofolate Reductase) gene

Abstract

The invention provides a primer, probe, fluorescent PCR kit and method for detecting nucleotide polymorphisms of two sites of c.665 and c.1286 in an MTHFR (Methylene Tetrahydrofolate Reductase) gene. The nucleotide types of the two polymorphic sites of c.665 and c.1286 in the MTHFR gene are detected on a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) technology platform according to the principle of 'allelic specific PCR'.

Description

technical field [0001] The invention belongs to the field of biomedical clinical molecular detection, and specifically relates to a primer, a probe, a fluorescent PCR kit and a detection method for detection of nucleotide polymorphisms at two sites c.665 and c.1286 of the MTHFR gene. Background technique [0002] Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in the methionine-folate metabolic system, which can reduce 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, On the one hand, it can participate in the synthesis of purine and pyrimidine and the methylation of DNA, RNA and protein as an indirect donor of methyl group; on the other hand, under the catalysis of methionine synthetase, vitamin B12 is used as coenzyme to make Homocysteine ​​in the blood undergoes methylation to produce methionine, thereby maintaining normal homocysteine ​​levels in the body. Mutations in the MTHFR gene can reduce the activity of key enzymes encoding folic acid metabo...

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies such as high price, long period, low efficiency, cumbersome data analysis and the like when detecting the MTHFR gene polymorphism in the prior art, the present invention provides a kind of primer and fluorescence detection method based on the real-time fluorescence quantitative PCR technology platform. The probe, kit and detection method are used to detect the c.665 site (C / T) polymorphism and / or the c.1286 site (A / C) polymorphism of the human MTHFR gene. Low, with extensive promotion value

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