Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Liquid crystal biosensing method for detecting UO2<2+>

A biosensing and liquid crystal technology, applied in measuring devices, material analysis through optical means, instruments, etc., can solve the problems of precision and expensive equipment, cumbersome sample pretreatment, etc., and achieve the effect of simple operation and good specificity

Inactive Publication Date: 2017-06-13
HUNAN UNIV
View PDF1 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most methods for detecting U mainly rely on instrumental analysis methods. According to the unique properties of the U element itself, there are mainly atomic absorption spectrometry, inductively coupled plasma method, potentiometric titration, spectrophotometry, fluorescence method and Raman spectroscopy. etc. Most instrumental analysis methods require tedious sample pretreatment, and the required equipment is sophisticated and expensive

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Liquid crystal biosensing method for detecting UO2&lt;2+&gt;
  • Liquid crystal biosensing method for detecting UO2&lt;2+&gt;
  • Liquid crystal biosensing method for detecting UO2&lt;2+&gt;

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of standard solution

[0026] Tris-HCl buffer (pH 8.0): 20mM Tris-HCl, 50mM NaCl, 100mM Mg 2+ , 10mM NaBH 3 CN.

[0027] 6×SSC stock washing solution (pH 7.0): 0.9M NaCl, 0.09M trisodium citrate dihydrate, 0.01% sodium dodecyl sulfate; when used, dilute to 1×SSC with ultrapure water (used to remove non-specific Adsorbed DNA).

[0028] MES buffer (pH 5.5): 50mM MES, 300mM NaCl

[0029] UO 2 2+ Preparation of standard solution: accurately weigh 42.4mgUO 2 (CH 3 COO) 2 Dissolve in sterile water in a 10ml centrifuge tube and store in the dark.

[0030] UO 2 2+ Working solution: Take a certain volume of UO 2 2+ The standard solution was diluted to the required concentration with MES buffer solution.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention provides a liquid crystal biosensing method for detecting UO2<2+>. The liquid crystal biosensing method comprises the steps of firstly modifying a TEA / DMOAP mixed self-assembling film on the surface of a sensing substrate, fixing a capture probe to the surface of a lower slide substrate modified by virtue of a chemical sensitive film, then dropwise adding a mixed solution obtained through reaction of UO2<2+> and a DNA enzyme double chain compound, assembling an upper slide modified with a DMOAP sensitive film and a lower slide modified with a TEA / DMOAP mixed sensitive film and linked with DNA face to face so as to form a liquid crystal box, and applying the liquid crystal box to a polarizing microscope so as to observe optical signal response. According to the liquid crystal biosensing method for detectinf UO2<2+>, a simple and visual liquid crystal biosensing method is developed, and the detection of UO2<2+> is realized. The method has the advantages that the operation is simple, the specificity is good, the marking is not required, and the like.

Description

technical field [0001] The invention belongs to the field of liquid crystal biosensors, in particular to a method for detecting UO 2 2+ liquid crystal biosensing method. Background technique [0002] Liquid crystal (LC) is a special state of matter that is different from solid, liquid, and gaseous states within a certain temperature range. It has both the birefringence characteristic of anisotropic crystals and the fluidity of liquids. , can respond sensitively to the stimuli of external conditions, and can convert interface events into optical responses that can be observed only with a polarizing microscope. Liquid crystals have emerged as a powerful tool for monitoring interfacial phenomena at solid-phase interfaces and liquid crystal-water phase interfaces. The sensing mechanism of the solid phase interface is mainly based on the fact that the self-assembled sensitive film fixed on the surface of the sensing substrate can recognize the changes in spatial conformation a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/23
CPCG01N21/23
Inventor 吴朝阳李萍杨胜园肖福兵
Owner HUNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com