The invention discloses a kit for detecting
methylation of a
lung cancer-associated
gene SHOX2 (short stature homebox2). Methylated loci of target genes in a
free DNA (deoxyribonucleic acid) are detected, and the kit comprises primers corresponding to three methylated target genes and an internal
reference gene primer, and preferably further comprises MGB (
minor groove binder) probes corresponding to methylated loci of the three target genes, an
MGB probe corresponding to an internal
reference gene and MGB probes corresponding to the three methylated target genes. The detection loci comprise
promoter areas of the genes and coding areas of the genes, and multi-area detection is favorable for improving the
methylation accuracy and specificity of the SHOX2. The methylated loci are preferably detected more accurately by specifically combining the MGB probes and a
methylation sequence. The kit is convenient to operate and easy to read, and apparatus requirements are low; a complete sealing form is adopted for the whole PCR (
polymerase chain reaction) process, so that the probability of
cross infection is avoided, and a result is more accurate. The kit with high detection sensitivity is applied to early screening of
lung cancer.