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Human tumor-related methylation site as well as screening method and use thereof

A tumor-related, screening method technology, applied in the field of endometrial cancer diagnosis and detection, can solve the problem that the potential role of endometrial cancer has not yet been studied.

Active Publication Date: 2017-09-22
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since changes in DNA methylation are involved in the occurrence and development of tumors, early diagnosis and prevention of tumors can be performed by detecting changes in DNA methylation caused by BMI. Potential role in the development and progression of endometrial cancer has not been investigated

Method used

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  • Human tumor-related methylation site as well as screening method and use thereof
  • Human tumor-related methylation site as well as screening method and use thereof
  • Human tumor-related methylation site as well as screening method and use thereof

Examples

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Embodiment 1

[0044] This embodiment provides a method for screening human tumor-associated methylation sites, comprising the following steps,

[0045] (1) Endometrial cancer samples are selected as tumor samples, and the BMI clinical information and DNA methylation data of the endometrial cancer samples are provided by the TCGA database, and the BMI is 25kg / m 2 As a threshold for grouping endometrial cancer samples, a BMI of less than 25 kg / m 2 Endometrial cancer samples were used as the control BMI group, and the BMI was greater than or equal to 25kg / m 2 The endometrial cancer samples were taken as the high-level BMI group;

[0046] (2) Using the limma software package to correct the three confounding factors of age, race and tumor sampling point in the control BMI group and the high-level BMI group of endometrial cancer samples in step (1), and calculate the significant differential methylation site, FDR Figure 1-3 as shown, figure 1 The differences in methylation sites between the en...

Embodiment 2

[0053] This embodiment provides a prognostic marker for endometrial cancer, including at least one of cg12645852, cg0962147 and cg14044785 obtained in embodiment 1. Further, the nucleotide sequence of the methylation site cg12645852 is shown in SEQ ID NO.1, and the nucleotide sequence of the methylation site cg09621472 is shown in SEQ ID NO.2 , the nucleotide sequence of the methylation site cg14044785 is shown in SEQ ID NO.3.

Embodiment 3

[0055] This embodiment provides a primer for the prognosis and diagnosis of endometrial cancer, which is designed based on the markers for the prognosis and diagnosis of endometrial cancer described in Example 2.

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Abstract

The invention provides a human tumor-related methylation site as well as a screening method and use thereof. The human tumor-related methylation site is selected from at least one methylation site on a human 18th chromosome, a human third chromosome and a human second chromosome; the change of methylation site, caused by BMI, on chromosomes is related to a human endometrial cancer; prognosis on a human tumor can be performed by detecting the methylation site changes on the chromosomes; the human tumor-related methylation site can be used as a potential prognosis marker of the tumor, and especially can be used as a prognosis marker of the human endometrial cancer.

Description

technical field [0001] The invention belongs to the technical field of diagnosis and detection of endometrial cancer, and in particular relates to a human tumor-related methylation site and its screening method and application. Background technique [0002] Endometrial cancer is a group of epithelial malignant tumors that occur in the endometrium, and it is more common in perimenopausal and postmenopausal women. Endometrial cancer is one of the most common female reproductive system tumors, with nearly 200,000 new cases each year, and is the third most common gynecological malignancy leading to death. Due to the complex pathogenic factors and unknown pathogenesis, early diagnosis and detection of endometrial cancer are very difficult, and endometrial cancer is often found to be in the middle and advanced stages, causing patients to miss the best opportunity for endometrial cancer treatment. [0003] In recent years, studies have shown that DNA methylation can be used as a b...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/154
Inventor 高山顾殷敏张卫红
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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