Optical fiber sensing micro-fluidic chip nucleic acid amplification in-situ real-time detection system and method

Abstract

The invention relates to an optical fiber sensing micro-fluidic chip nucleic acid amplification in-situ real-time detection system and method. The system comprises a white light source, a detecting light path, a micro-fluidic chip and a spectrum collecting, processing and display module which are sequentially connected, wherein the detecting light path is used for transmitting white light generated by the white light source to the micro-fluidic chip and transmitting optical signals passing the micro-fluidic chip to the spectrum collecting, processing and display module; the micro-fluidic chipis used for performing biochemical reaction; the spectrum collecting, processing and display module is used for collecting the optical signals passing the micro-fluidic chip, analyzing the signals andgenerating a visual biochemical reaction real-time dynamic-change signal curve. The system has the advantages that a method using white light to interfere hyper-spectrum is used to detect nucleic acid amplification information, both a fluorescently-labeled to-be-detected object and a to-be-detected object which is not fluorescently labeled can be detected, and the problems that a fluorescent labeling method affects biological reaction activity, and fluorescence decay cancellation is instable are solved.

Description

technical field [0001] The invention relates to an in-situ real-time detection system and method for nucleic acid amplification of an optical fiber sensing microfluidic chip, and belongs to the technical field of biological detection. Background technique [0002] Nucleic acid amplification is an advanced biomedical analysis technology in the 20th century, which can amplify specific sequence nucleic acid fragments to 10 within 120 minutes 9 copy number for highly sensitive molecular diagnostics. Nucleic acid amplification techniques mainly include variable temperature amplification and isothermal amplification. [0003] Variable temperature amplification is represented by PCR (Polymerase chain reaction), which is the earliest nucleic acid amplification technology. The nucleic acid amplification reaction is started by the complete matching of the positive and negative specific primers. Each cycle of amplification cycle is about 90 seconds, including denaturation, Three stag...

AI Technical Summary

Problems solved by technology

The actual sensitivity of fluorescence detection is 10 3 More than one nucleic acid copy, the instrument is expensive, the consumption of sample reagents is large, and the detection cost is high, which is not conducive to the low-cost precision medical analysis and detection application of multi-indicator combination

In addition, there are also a small number of nucleic acid amplification instruments that use turbidity measurement methods for detection, but their detection sensitivity is not high, and the detection results are not as accurate as fluorescence detectors, so they can only be used in occasions that do not require high experimental accuracy, such as qualitative analysis of the end point

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