55 results about "Molecular Technique" patented technology

A process is described for producing hybrid seed using male-sterile plants created by employing molecular techniques to manipulate antisense DNA and other genes that are capable of controlling the production of fertile pollen in plants. Transformation techniques are used to introduce constructs containing antisense DNA and other genes into plants. Said plants are functionally male-sterile and are useful for the production of hybrid seed by the crossing of said male-sterile plants with pollen from male-fertile plants. Hybrid seed production is simplified and improved by this invention and can be extended to plant crop species for which commercially acceptable hybrid seed production methods are not currently available.

The invention relates to feature sequences and a molecular specificity tag primer of a pair of high-specificity feature sequences of carya illinoensis variety Nacono, a molecular specificity tag primer, and a method capable of being used for fast identifying the carya illinoensis variety Nacono. The molecular specificity tag primer has the sequence that a forward primer is 5'-GTCACTTCCCTTTCTCGGCA-3', and a reverse primer is 5'-CCCCGGGTGCAGGTATAAAA-3'. The molecular specificity tag primer provided by the invention can be used for performing fast early-stage identification on the carya illinoensis variety Nacono; the method is simple, fast and accurate, and belongs to an irreplaceable molecular technique for distinguishing the carya illinoensis variety through appearance features.

The invention relates to feature sequences and a molecular specificity tag primer of a pair of high-specificity feature sequences of carya illinoensis variety Van Deman, a molecular specificity tag primer, and a method capable of being used for fast identifying the carya illinoensis variety Van Deman. The molecular specificity tag primer has the sequence that a forward primer is 5'-ATGCAAGACGCTTGTGGAGA-3', and a reverse primer is 5'-CCGACCCGACCCGATAAAAT-3'. The molecular specificity tag primer provided by the invention can be used for performing fast early-stage identification on the carya illinoensis variety Van Deman; the method is simple, fast and accurate, and belongs to an irreplaceable molecular technique for distinguishing the carya illinoensis variety through appearance features.

The invention relates a method for identifying a structure of a yeast colony of a Daqu starter or a fermented grain of distilled spirit by using denaturing gradient electrophoresis, which belongs to the field of microbial ecological techniques. The method comprises the following steps: extracting a genome DNA of the Daqu starter or fermented grain sample of the distilled spirit by using a bead milling method; by designing a general DGGE primer of yeasts in a common wine-making microbe, performing PCR amplification on a specific specificity DNA segment in a yeast ribosome; separating corresponding DNA segments of different varieties by DGGE electrophoresis; gel-cutting and recovering straps corresponding to preponderant microbes; performing the PRC application again, connecting a T carrierand selecting a positive clone; and after re-comparison by the DGGE electrophoresis, checking orders and identifying microbe information corresponding to gel-cutting straps. The method adopts a molecular technique without depending on cultivation, and has the characteristics of simplicity, convenience, quick speed, sensitivity and the like; besides, the method provides a detection method for identifying the structure of the yeast colony of the Daqu stater or the fermented grain of the distilled spirit, and further provides a reference frame for developing wine-brewing microbes oriented to distilled spirit flavors with different odor types.

The invention discloses a molecular attribute determination method and device, electronic equipment and a storage medium, belonging to the technical field of molecules. The method comprise the following steps: S1, representing molecular attribute data of each labeled molecule and molecular structure data of each unlabeled molecule by using molecular topological structure diagrams; S2, inputting the molecular topological structure diagrams of all the labeled and unlabeled molecules into a pre-constructed teacher model, and training all the molecular topological structure diagrams by utilizing semi-supervised learning to obtain parameters of a teacher model; S3, migrating the parameters of the teacher model to a pre-constructed student model; S4, adjusting the student model by using labeledmolecules; S5, using the adjusted student model to assign labels to all the unlabeled molecules to obtain a labeled molecule set; S6, feeding back the labeled molecular set to the teacher model; and S7, repeatedly executing the steps S2 to S6 until the teacher model and the student model are both converged, and predicting molecular attributes by utilizing the converged student model. According tothe invention, the attributes of unknown molecules can be accurately predicted.

The invention relates to the field of estimating a microbial count in a sample using molecular techniques. The invention furthermore relates to a kit for performing such estimation and use of the estimation method for providing bacterial cell counts in milk and for diagnosing infectious conditions in animals and human beings. Furthermore, the invention relates to a method for quality control of food (especially milk and dairy products), feed and water samples using molecular techniques. Reproducible and reliable estimates of a microbial count can be obtained using a molecular hybridisation assay between a target nucleic acid sequence and a labelled probe. Proper calibration and quantitative recording of the signal produced by the label can estimate a microbial count. One important feature of the invention is the use of locked nucleic acid (LNA) monomers in the probes.

The invention uses a universal primer for amplification to obtain 66 cardiidae mtCOI (cytochrome c oxidase I) sequences, uses a CLUSTALW software to compare and analyze, uses primer design software primer Premier5 to design 5 pairs of primers in a determined area, uses 216 cardiidae individuals to repeatedly conduct FCR reaction tests in a 10mu m system, finally is detected through agarose gel electrophoresis, and screens out ctockle shellfish mitochondria COI gene sequence efficient amplification primer. The cockle shellfish mitochondria COI gene amplification primer is characterized in that NGF5'-ACAAATCATAAAGATATTGG-3'NGR5'-TTCAGGGTGTCCGAAGAATCA-3' adopts the cockle shellfish mitochondria COI primer, objective sections of different species of cockle shellfish are effectively amplified, requirements of genetic diversity survey of cockles can be met, simultaneously reliable molecular technique instruments for germplasm resource protection and breeding industry development are provided.

The present invention discloses a method for constructing a yeast expression vector and preparing cellobiohydrolase by the yeast expression vector and an application thereof. A modern molecular technology successfully optimizes a gene sequence encoding the shii-take cellobiohydrolase, constructs the expression vector pPICZ alpha A-Cel7A of the cellobiohydrolase by expressed by yeasts in vitro, amdthe vector and pichia pastoris are utilized to prepare the cellobiohydrolase and thus provide technical supports for an application of the cellobiohydrolase in production. The recombinant pichia pastoris obtained by the method is high in enzyme yield, high in activity and excellent in effects.

The invention discloses a primer probe combination for identifying mycobacterium abscessus and mycobacterium massiliense. The primer probe combination provided by the invention consists of a single chain DNA shown as sequences 1 to 4 in a sequence table. The invention provides an identification method of mycobacterium abscessus and mycobacterium massiliense; the distinguishing on the mycobacteriumabscessus and the mycobacterium massiliense can be fast completed in the molecular level; the characteristics of simplicity, convenience, high speed, high specificity and high sensitivity are realized, so that the problem that the mycobacterium abscessus composite groups cannot be accurately and fast identified by the existing molecular technology is solved.

The invention belongs to the technical field of molecules, and particularly relates to a method for detecting environment DNA of giant salamander distribution. According to the specific technical scheme, according to the method for detecting the environment DNA of giant salamander distribution, at least one pair of primers is used for conducting PCR amplification detection on a to-be-detected sample, if 158-224 bp positive bands appear in an electrophoresis result, it is considered that giant salamanders are distributed in the environment where the to-be-detected sample is sourced, the primer pair is AdCytbE1, and the sequences are SEQ ID NO: 1 and SEQ ID NO: 2. According to the method, environmental DNA of excrement, skin exfoliates, corpses and the like of Chinese giant salamanders in residual water is utilized, a molecular biological technology is combined, a primer with high specificity, high sensitivity and strong universality is developed, and a method capable of carrying out rapid and standardized detection aiming at the distribution of different species of giant salamanders is established.

The invention provides a primer probe set for rapidly and efficiently detecting candida aurantiaca based on fluorescent PCR technology as well as a kit and application thereof, and belongs to the technical field of pathogenic microorganism in-vitro molecules. The invention relates to a specific DNA fragment for detecting the candida aurantiaca, wherein the DNA fragment has a nucleotide sequence shown as SEQ ID NO: 1. The primer probe set for rapidly and efficiently detecting the candida aurantiaca based on the fluorescent PCR technology comprises a primer pair, and a probe; the primer pair consists of a forward primer with a nucleotide sequence as shown in SEQ ID NO: 2, and a reverse primer with a nucleotide sequence as shown in SEQ ID NO: 3; and the probe has a nucleotide sequence as shown in SEQ ID NO: 4. The primer probe set and the kit thereof are capable of rapidly and efficiently detecting the candida aurantiaca on basis of the fluorescent PCR technology; and thus, high detectionsensitivity, short detection time and simple operation can be realized.

The invention relates to a directed molecule generation method based on a graph neural network, and relates to the technical field of material molecules. Comprising the following steps: converting an organic molecular structure graph into a molecular graph in a topological mapping mode, and taking embedded representation of the molecular graph as input of a graph neural network model; through a graph neural network model, learning the molecular graphs based on a message propagation process, including representations of nodes and edges in the molecular graphs; the generated representations are learned through a graph neural network so that various decisions can be made in the graph generation process; in the decision-making process, the new structure is added to the existing graph in a form conforming to the organic molecule chemical rule, and the probability of the addition event depends on the historical graph derivation process of the graph. The finally generated novel molecules are confirmed through chemical valence constraint, and the chemical effectiveness of the generated molecules can be ensured. According to the method, effective novel molecular structures with chemical properties similar to those of original molecules can be generated aiming at an organic molecule database.

The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping.

The invention discloses a cancer marker detection method combining DNA coding technology and nanopore technology. The cancer marker detection method comprises the following steps: firstly, synthesizing a DNA coding molecule, and modifying the group and the length of DNA by virtue of chemical modification changing the DNA base, so as to generate a characteristic current signal with higher discrimination when the DNA coding molecule passes through nanopores; in combination with immunoassay technology, adding cancer markers to form a sandwich structure with magnetic beads and nano gold; and thenreleasing the encoded DNA on the surface of nanogold, so as to detect the cancer markers separately and simultaneously. A high-throughput multi-component detection platform based on nanopore single molecule technology outputs corresponding characteristic current signals with different DNA coding molecules, so as to eliminate background interference as well as signal interference between the DNA coding molecules, and overcome the defects of fluorescent bleaching, short fluorescence lifetime, background interference, fluorescence interference between encoded microspheres and the like of fluorescence encoding of a traditional method.

The invention discloses an antibody detection method for combining a three-chain DNA molecular beacon with a nanopore technology for combination. The method comprises the steps that the three-chain DNA molecular beacon used for detecting an antibody is designed and synthesized, when the antibody is combined with the three-chain DNA molecular beacon, the molecular beacon structure is destroyed, anda probe is released; an antibody-antibody capture DAN molecular complex cannot penetrate through a nanopore due to too large volume, the probe passes through the nanopore and can generate a characteristic current signal, the signal specificity is extremely high, molecular recognition can be accurately achieved, and the background interference problem is avoided. In addition, the unique thermodynamics and kinetic properties of the molecular beacon are combined with the nanopore single molecular technique, and high sensitivity detection on the variable antibody is achieved. In addition, the method has the advantages that implementation is easy, reagent consumption is extremely low, and large-scale equipment is not needed.