Nymphaea tetragona DNA fingerprint, primer to acquire same and construction method of Nymphaea tetragona DNA fingerprint

Abstract

The invention relates to Nymphaea tetragona DNA fingerprint, a primer to acquire the same and a construction method of the Nymphaea tetragona DNA fingerprint and belongs to the technical field of biology. The construction method of the Nymphaea tetragona DNA fingerprint comprises the specific steps of 1) extracting Nymphaea tetragona DNA; 2) performing ISSR-PCR (inter-simple sequence repeat-polymerase chain reaction) amplification reaction and electrophoresis detection, to be specific, amplifying Nymphaea tetragona DNA with an ISSR primer, and electrophoretically detecting the ISSR-PCR productwith 1.8% agarose gel, wherein the ISSR primer is from one or more of UBC840, UBC841, UBC811, UBC834, UBC835, UBC843, UBC844, UBC845, UBC873, and UBC880; 3) constructing DNA fingerprint. A stripe amplified with a Nymphaea tetragona ISSR-PCR system established herein has good repeatability and high polymorphism; the defects of domestic studies on Nymphaea tetragona germplasm resource genetic diversity and molecular mapping are overcome. The Nymphaea tetragona DNA fingerprint constructed via the method herein can act as basis to identify and classify corresponding primary germplasm resources ofNymphaea tetragona, and also guarantee the classification and identification of new germplasms and new varieties and the protection of intellectual properties at molecular technical level.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to water lily DNA fingerprints, primers and construction methods thereof. Background technique [0002] Water lilies are perennial herbaceous perennial flowers of the genus Nymphaea L. in the family Nymphaeaceae. Water lily germplasm resources are the material basis for the development of fresh cut flowers and edible production of water lilies, and are also the basis for the field of water lily research, especially the breeding of new water lily varieties and biotechnology research. At present, the production and management of flower variety resources are not standardized in our country, and the phenomenon of variety introduction confusion or variety fraud occurs from time to time. Therefore, it is particularly important to carry out identification of water lily variety resources. The traditional water lily species resource identification method mainly relies on phenotypic ...

AI Technical Summary

Problems solved by technology

At present, the production and management of flower variety resources in my country is not yet standardized, and the phenomenon of variety introduction confusion or variety fraud occurs from time to time. Therefore, it is particularly important to carry out identification of water lily variety resources

The traditional water lily species resource identification method mainly relies on phenotypic characteristics. Although it is fast and convenient, the phenotypic traits are greatly affected by the environment, and the identification error rate is high. making it more and more difficult to pass traditional phenotyping methods

[0003] Although the research on water lilies in my country is in its infancy, it is developing rapidly. At present, the research on water lilies mainly focuses on the introduction and cultivation, reproduction and breeding, functional activity, physiological and biochemical analysis, and selection of new varieties. The genetic diversity and molecular maps of water lily germplasm resources There are few studies reported

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