58results about How to "Eliminate cumbersome steps" patented technology

The present invention provides methods, compositions and kits for isolating and purifying at least one nucleic acid directly from a biological sample, or for preparing a biological sample for subsequent isolation and purification of at least one nucleic acid, without removal of the biological sample's cell culture medium or cellular fluid.

The invention relates to a laser detection system for oxygen concentration in tail gas of a vehicle-mounted type motor vehicle. The laser detection system comprises an optical absorption tank and a tunable semiconductor laser absorption spectrum detection device, and is characterized in that the optical absorption tank is a diffuse reflection optical absorption tank; laser beams output by the tunable semiconductor laser absorption spectrum detection device are transmitted to an optical fiber flange through optical fibers and penetrate through a window mirror to be reflected into a diffuse reflection tank; in the diffuse reflection tank, the laser beams are diffusedly reflected to a focusing lens through a diffuse reflection mirror and then are transmitted into the tunable semiconductor laser absorption spectrum detection device through a photoelectric detector to carry out optical intensity normalization treatment on a second harmonic signal by a direct-current component; then an L-M algorithm is used for carrying out least square fit on a fitting function model; and a fitted second harmonic signal sequence is subjected to temperature correction to directly calculate the oxygen concentration in the tail gas of the vehicle-mounted type motor vehicle.

Techniques for measuring client-side performance include intercepting an item that is to be sent to a client process prior to the arrival of the item at the client process. The item is modified to produce a modified item that includes code. The code causes a processor on the client device to measure performance related to a service associated with the item. The code also causes the processor to perform one or more acts based on a measurement resulting from measuring performance. The modified item is sent to the client process. These techniques allow a service provider to automatically modify an item each time the item is sent to a client process, dispelling cumbersome steps in installing and maintaining software on a client device. The modifications may be made transparent to a designer of the server application and thus easily applied to both new and existing server applications. These techniques also allow the service provider to obtain measurements of actual performance experienced by the real users and without placing a spurious load on the website. The code in the modified item can be tailored to provide any response desired, from reporting those measurements to the service provider for analysis by the service provider, to automatically notifying the user of the client process, to automatically diagnosing the cause of unfavorable performance such as by correlating performance problems with particular components of the client process.

The invention discloses a method for reproducing Bracon adoxophyesi Mimanikawa indoor. Under the conditions that the temperature is 25 DEG C, and the ratio of light to darkness is 14 h to 10 h, the Bracon adoxophyesi Mimanikawa is placed into an insect rearing cage with a sleeve, wherein the specification is 55 cm*55 cm*55 cm, the Bracon adoxophyesi Mimanikawa is fed by 15% of hydromel, fodder with insects is taken out from a feeding plate with 5-6-instar rice moth larvae every 3 days to be smoothly laid at the bottom of a plastic box to enable the height of the fodder with the insects to be approximately 1.5 cm, the plastic box without a cover is placed in a parasitoid wasp insect rearing cage, and the parasitoid wasp utilizes the rice moth as the host. The plastic box is taken out 3 days later, and the plastic box is covered by the cover, the emergence of the parasitoid wasp of the next generation begins to occur after 12 days, and the emergence time lasts more than 7 days. The method is simple in operation and high in efficiency, and the mass reproduction of the Bracon adoxophyesi Mimanikawa can be achieved easily.

The invention relates to a method for designing, amplifying and sequencing twelve pairs of floccularia luteovirens microsatellite primers. The method comprises the following steps: (1) extracting genome DNA of three floccularia luteovirens populations among which the geographic interval is more than 300km by using an improved CTAB method; (2) randomly selecting an individual genome DNA respectively from the three populations, mixing, detecting the quality of total DNA, preparing a gene library, and performing Illumina HiSeq<TM>2500 sequencing after the gene library is qualified in examination in depot; (3) splicing sequenced data, detecting simple sequence repeats (SSR) in the total DNA sequence by using SR search software, and performing primer design by applying primer3; (4) preparing an SSR primer having an annealing temperature of 50-60 DEG C by adopting a temperature gradient method; (5) respectively performing PCR amplification on the genome DNA of the three floccularia luteovirens populations, and sequencing and verifying to obtain 12 pairs of primers with polymorphism; and (6) calculating the number N of allelic genes, haplotype diversity H<d> genetic differentiation coefficient F<ST>, nucleotide diversity P, G<ST> and the value of pi. The method is beneficial to large-scale research.

An interior permanent magnet (IPM) rotary machine comprises a rotor comprising a rotor yoke having bores and a plurality of permanent magnet segments disposed in the bores of the rotor yoke, each permanent magnet segment consisting of a plurality of magnet pieces. The rotor is assembled by inserting the plurality of unbound magnet pieces in each bore for stacking the magnet pieces, and fixedly securing the stacked magnet pieces in the bore.

The invention discloses a humanized single-chain antibody 8B of clostridium perfringens alpha-toxin. The humanized single-chain antibody 8B is screened from a phage antibody library Source bioscience, is a full-humanized antibody of the clostridium perfringens alpha-toxin and can be used for achieving a toxin neutralization treating effect, overcoming various side effects of a heterologous antibody and eliminating complex steps and high cost of humanization modification on the heterologous antibody; and the single-chain antibody is small in molecular weight, strong in in-vivo penetrability and capable of rapidly reaching to damaged tissues and cells to exert an antitoxin effect, so that the double aims of economy and high efficiency are achieved. The alpha-toxin has a certain homology with other types of toxins of clostridium perfringens, so that the antibody drug possibly generates inhibiting and treating effects for other types of toxins and can also be used as a detecting reagent to detect the clostridium perfringens alpha-toxin.

A method according to the present invention is a method for producing an ionic liquid from a molten salt solution containing a molten salt constituted by combining the cation portion and the anion portion, the ionic liquid containing the molten salt as a main component, the method comprising: removing water from the molten salt solution, the step of removing the water including adding a compound in the molten salt solution, the compound being represented by General Formula (1):where at least two of R1 to R4 are a C1 to C8 alkoxy group(s), the rest of R1 to R4 are a hydrogen atom(s) or a C1 to C8 alkyl group(s). By this, it is possible to remove water from the molten salt solution regardless of whether the molten salt solution is hydrophilic or hydrophobic. The present invention provides a method of producing an ionic liquid, which method can remove water from any kind of ionic liquid economically, easily, and quickly.

An interior permanent magnet (IPM) rotary machine comprises a rotor comprising a rotor yoke having bores and a plurality of permanent magnet segments disposed in the bores of the rotor yoke, each permanent magnet segment consisting of a plurality of magnet pieces. The rotor is assembled by inserting the plurality of unbound magnet pieces in each bore for stacking the magnet pieces, and fixedly securing the stacked magnet pieces in the bore.

The invention relates to a cell extraction method in the technical field of bioengineering, in particular to an extraction method for chick embryo myoblasts. The method includes the following steps that firstly, chick embryos are sterilized, and pectoralis major is taken and put into a D-Hank's culture dish; secondly, the pectoralis major is trimmed; thirdly, the pectoralis major is oscillated and suspended through a vortex oscillator; fourthly, filtration and centrifugation are carried out; fifthly, cell plate laying and differential attachment are carried out; sixthly, digestion is carried out to obtain the myoblasts; seventhly, counting and plate planting are carried out. By means of the extraction method for the chick embryo myoblasts, namely, the mechanical oscillation extraction method for the chick embryo myoblasts, it is guaranteed that the purity, number and vitality of the extracted myoblasts are higher than those of myoblasts extracted through other methods, and a foundation is laid for further development of poultry muscle development regulation and control mechanism experiments.

The invention provides a server cabinet, which comprises a cabinet body, a cabinet door, an evaporator, an evaporator fan, a condenser, a condenser fan, a compressor, an expansion valve and a shock absorbing device, and is characterized in that the cabinet body is provided with a first cavity, the first cavity is provided with a return air inlet and an air outlet, the cabinet door is hinged on the cabinet body, the cabinet door is provided with a second cavity and a third cavity, the evaporator is installed in the second cavity, the evaporator is located at the air outlet, the evaporator fan is installed in the second cavity, the evaporator fan is located at the return air inlet, the condenser is fixedly installed in the third cavity, the condenser fan is fixedly installed in the third cavity, the compressor is installed in the third cavity, a suction end of the compressor is connected with the evaporator, a discharge end of the compressor is connected with the condenser, the expansion valve is connected between the evaporator and the condenser, and the shock absorbing device is connected between the compressor and the cabinet door. The server cabinet has the advantages of good heat exchange effect, low energy consumption, low vibration and possession of an independent refrigerating system.

Provided herein is a single component sealant formulation (e.g. in a liquid form), methods for its preparation, and use. The formulation includes fibrinogen; vitamin K-dependent clotting zymogens comprising at least Factor II (FII) and Factor X (FX).

The invention provides a radar mounting structure and a vehicle with same. The radar mounting structure is used for fixedly configuring a vehicle radar; the radar mounting structure comprises a wave absorbing sheet, a radar fixing bracket and a vehicle body fixing bracket; the vehicle radar is arranged in the radar fixing bracket; the wave absorbing sheet is made of a wave absorbing material, arranged around the vehicle radar and fixedly clamped to the radar fixing bracket so as to reduce the interference of electromagnetic waves on the vehicle radar; the vehicle body fixing bracket is fixed on the other side, opposite to the vehicle radar, of the radar fixing bracket; and the vehicle body fixing bracket is used for fixedly assembling the radar mounting bracket on a vehicle body. Through adoption of the radar mounting structure, the production cost can be reduced while the vehicle radar is prevented from being interfered by surrounding electromagnetic waves; and platform sharing of different vehicle body environments is realized.

The invention discloses an elastic push-and-press type high-voltage fuse tube base. The elastic push-and-press type high-voltage fuse tube base comprises a cylindrical hollow guide tube, a guide tubeend cap and a contact slide plate, wherein the cylindrical hollow guide tube is used for installing a fuse tube; the guide tube end cap is fixed onto a fixing frame; the hollow guide tube is in threaded connection with the guide tube end cap; a push spring is arranged between the contact slide plate and the guide tube end cap; and the contact slide plate is slidingly connected onto the inner wallof the hollow guide tube. With the elastic push-and-press type high-voltage fuse tube base of the invention adopted, problems such as the problem of damage to a high-voltage fuse tube due to the disengagement of the high-voltage fuse tube which is caused by the vibration of the body of a voltage transformer handcart or the problem of heat emission and discharge which is caused by poor contact during the push and pull operation process of the high-voltage fuse tube of the voltage transformer handcart can be fundamentally eliminated; the reliability of the fixation and contact of the high-voltage fuse tube can be improved; and the use service life of the high-voltage fuse tube fixing structure can be ensured. The elastic push-and-press type high-voltage fuse tube base is convenient to use, simple to manufacture, low in cost and high in applicability.

The invention discloses a U disc capable of being encrypted. The U disc comprises a U disc control module used for data monitoring and processing, a storage module used for data storage, a virtual file distribution and sector remapping module used for generating a disc distribution list, and a data encryption module used for encryption processing of sent files, the disc distribution list comprises a common catalog and a preset interface catalog, and when it is detected that a host has write-in operation aiming at the preset interface catalog, the files are sent to the data encryption module. According to the U disc capable of being encrypted, the defect that the operation of special application software is needed during file copying is overcome, the input of passwords is not required, complicated and inconvenient operation of mounting an encryption partition etc. is not needed, and encryption storage of the files can be realized.

The invention discloses a system and method for authentication of dynamic voice ciphers in combination with any gestures. The system comprises a gesture registration terminal, a voiceprint registration terminal, a server and a client, wherein the gesture registration terminal comprises a gesture registration terminal network communication module, a display module and a gesture recording module, and the voiceprint registration terminal comprises a voiceprint registration terminal network communication module, a recording module and a display module. During an authentication process, the required voice steps may not be increased, and the identification performance is even higher, and the entire authentication process can be completed in 2-3s. User experience is good.

The invention provides a mechanical processing method of a wire arranging hole of a large-size mold cavity board, and belongs to the field of mechanical processing methods of wire arranging holes of large-size combined welding mold cavity boards. The method is a new method for re-determining core machining parameters of a trapezoidal removing area based on Y-direction coordinates of a central vertical surface of an acute-angle semicircular groove; the actual processing position parameters, re-determined based on the coordinates of the central vertical surface of an inherent acute-angle semicircular groove of the mold cavity board, of four boundary lines of a rectangle corresponding to the Z-direction lower end surface S of a general trapezoidal quadrangular removing area or the actual processing position parameters of four boundary lines of a rectangle corresponding to the Z-direction lower end surface S' of a right trapezoid quadrangular removing area are treated as new processing size control parameters of numerical control milling machine. Therefore, the side edges of a determined processing area avoid each vertical rib, and as a result, the vertical ribs are prevented from damage.

The invention discloses a method for implementing an email agent system based on a Reply-to mail header. The method comprises the following steps: creating a mailbox group in a post office system database, enabling a post office system to randomly deliver a mail to a mailbox of any one mailbox group member in the mailbox group, and adding 1 to the number of received mails of the member; and when the mailbox group member replies to the mail, adding 1 to the number of replied mails of the mailbox group member, and changing a sender of the mail into a service number, so that the mail is displayedto a user at a client by a service number email address. According to the method for implementing the email agent system based on the Reply-to mail header provided by the invention, a mail group concept is completely transparent, and the post office system can also automatically calculate the number of the mails received by the mailbox group member and the number of the replied mails, which facilitates the management work of the mail processing of a worker; and meanwhile, the mails of the user can be efficiently processed in time, and good experience is provided for the user.

The invention discloses a medical digital X-ray system capable of realizing intelligent positioning. The medical digital X-ray system comprises an intelligent positioning processing unit and X-ray shooting equipment; the intelligent positioning processing unit comprises a computer, a database server, a human body feature processing device, a controller and an analysis module, the computer is connected with the database server through the Internet, the computer is in input connection with the human body feature processing device, the computer is in bidirectional connection with the analysis module, and the computer is in output connection with the controller; the intelligent positioning processing unit is connected with the X-ray shooting equipment through the controller; and the X-ray shooting equipment comprises a bulb tube suspension device, a chest radiography frame and a lifting bed, wherein the bulb tube suspension device is matched with the chest radiography frame for use or the bulb tube suspension device is matched with the lifting bed for use. According to the invention, intelligent positioning can be realized on the suspension type medical digital X-ray system, and the intelligent positioning does not bring redundant operation steps to an operator or a patient.

The invention discloses a humanized single-chain antibody 7D of clostridium perfringens alpha-toxin. The humanized single-chain antibody 7D is screened from a phage antibody library Source bioscience, is a full-humanized antibody of the clostridium perfringens alpha-toxin and can be used for achieving a toxin neutralization treating effect, overcoming various side effects of a heterologous antibody and eliminating complex steps and high cost of humanization modification on the heterologous antibody; and the single-chain antibody is small in molecular weight, strong in in-vivo penetrability and capable of rapidly reaching to damaged tissues and cells to exert an antitoxin effect, so that the double aims of economy and high efficiency are achieved. The alpha-toxin has a certain homology with other types of toxins of clostridium perfringens, so that the antibody drug possibly generates inhibiting and treating effects for other types of toxins and can also be used as a detecting reagent to detect the clostridium perfringens alpha-toxin.

The reusable multi-index microfluidic detection system comprises a microfluidic mixer, an ultrasonic transducer and a microfluidic detector which are arranged on a chip table, an electromagnet is arranged on the lower portion of the microfluidic detector, an optical detection unit is arranged on the upper portion of the microfluidic detector, and the optical detection unit is arranged on the chip table. The micro-fluidic mixer and the micro-fluidic detector are formed by bonding a PDMS (Polydimethylsiloxane) micro-channel and a glass substrate. Multi-index nucleic acid sequence / immunodetection can be carried out, tedious operation and large-scale detection equipment in a laboratory are replaced, the required sample amount is small, the detection time is short, magnetic beads marked by probe molecules are utilized, tedious steps of chip internal modification are omitted, the reaction speed and flexibility are improved, liquid phase reaction in a micro-channel is achieved, repeatability is good, precision is high, and the method is suitable for large-scale industrial production. Quantitative detection can be realized, and the chip can be reused.

The invention discloses a medical assistance method and system based on a data identification technology. The method is applied to the medical assistance system based on the data identification technology. The medical assistance system based on the data recognition technology comprises an electronic stethoscope used for collecting heart and lung sounds of a patient, a main control device used for processing data and terminal equipment used for displaying a result. The method comprises the following steps: collecting heart and lung sounds of a patient through an electronic stethoscope; and processing the heart and lung sound through the main control device, and displaying a processing result to a doctor through the terminal equipment to provide a diagnosis basis for the doctor. The electronic stethoscope of the system transmits collected cardiopulmonary sound to the terminal equipment after the cardiopulmonary sound is processed by the main control device in a wireless signal transmission mode, and the doctor can obtain information of the cardiopulmonary sound of a patient through the terminal equipment. Moreover, the electronic stethoscope is worn on the body of the patient, so that continuous and long-term heart and lung sound of the patient can be obtained.

The invention discloses a method for determining organochlorine pesticide residues in farmland soil through combination of ultrasound and gas chromatography. The method comprises the following steps:S1, sampling; S2, sample uniform mixing treatment; S3, extraction and salting-out; S4, oscillation mixing; S5, primary ultrasonic extraction; S6, secondary ultrasonic extraction; S7, primary concentration and purification; S8, secondary concentration and purification; S9, to-be-determined object making; S10, determination. The method has the advantages of being accurate in determination, high in sensitivity, simple and convenient to operate and the like. The chromatographic behavior study on DDT and metabolites thereof on a non-polar column HP-5 are also performed in the test, so that the accuracy is improved.

The invention discloses a special detection mask and method for a Raman immune probe for respiratory virus collection and detection. An area or an accessory sprayed with a Raman immune probe is attached to the melt-blown cloth layer on the inner side of the mask, and the area or the accessory is located at the position right corresponding to the mouth and the nose; the Raman immune probe adsorbs aRaman marker and a monoclonal antibody or a polyclonal antibody aiming at respiratory viruses, a solution of the Raman immune probe is directly sprayed on a melt-blown cloth layer or an accessory inthe middle of the mask, and an openable position is arranged on the inner side of the mask. The accessory sprayed with the Raman immune probe is detachably arranged at the openable position through anon-woven fabric combined with a magic tape, and the Raman immune probe is obtained by a special preparation method.

The invention discloses a fruit origin traceability detection method, device and equipment and a medium, and the method comprises the steps: detecting the origin of a to-be-detected fruit through employing a prediction model, and obtaining the origin information of the to-be-detected fruit, wherein the establishment process of the prediction model comprises the following steps: acquiring originalspectral data of target fruits of different producing areas and the same type by utilizing a near infrared spectrum analysis technology, and preprocessing the original spectral data to obtain preprocessed spectral data; setting a corresponding production place label for the preprocessed spectral data according to geographic position information in the preprocessed spectral data to obtain reconstructed spectral data; selecting training set data and test set data from the reconstructed spectral data; training the training set data based on a classification algorithm to obtain a training model; and if over-fitting and under-fitting do not exist in the training model, judging the training model as a prediction model. According to the method, the detection cost and the detection time required for tracing detection of the fruit production place can be remarkably reduced.

The invention discloses a robot and take-out cabinet interaction system and method, the robot and take-out cabinet interaction system comprises a take-out cabinet, two cargo boxes and a robot, two cabinet cavities and a box changing bin are arranged in the take-out cabinet, the box changing bin is located between the two cabinet cavities and is communicated with the two cabinet cavities, and a transfer seat is slidably arranged in the take-out cabinet; a locking mechanism which is locked with the container to achieve synchronous movement is arranged on the transfer seat, the container is a container with goods when the container contains goods, the container is an unloaded container when the container does not contain goods, and when the robot carries the unloaded container to move into the container changing bin, the locking mechanism is locked with the container to achieve synchronous movement. According to the system and the interaction method provided by the invention, the loading and unloading operations of the containers on the robot can be synchronously carried out, so that the cumbersome step of carrying out the two steps step by step in the prior art is eliminated, the loading and unloading efficiency of the containers is improved, the labor intensity of workers is reduced, and the working efficiency of the workers is improved. Meanwhile, the overall structure of the take-out cabinet is simplified, and the occupied space is reduced.

The invention relates to a cell extraction method in the technical field of bioengineering, in particular to an extraction method for chick embryo myoblasts. The method includes the following steps that firstly, chick embryos are sterilized, and pectoralis major is taken and put into a D-Hank's culture dish; secondly, the pectoralis major is trimmed; thirdly, the pectoralis major is oscillated and suspended through a vortex oscillator; fourthly, filtration and centrifugation are carried out; fifthly, cell plate laying and differential attachment are carried out; sixthly, digestion is carried out to obtain the myoblasts; seventhly, counting and plate planting are carried out. By means of the extraction method for the chick embryo myoblasts, namely, the mechanical oscillation extraction method for the chick embryo myoblasts, it is guaranteed that the purity, number and vitality of the extracted myoblasts are higher than those of myoblasts extracted through other methods, and a foundation is laid for further development of poultry muscle development regulation and control mechanism experiments.

The invention belongs to the field of TOPTIG welding technique methods for a butt pipe fitting of an antenna beam of a high-speed electric multiple unit (EMU), and provides a TOPTIG welding gun posture parameter value verification method for multi-layer welding of a butt joint of thick-walled pipes. The method is characterized in that requisite value ranges of a welding gun posture f (theta, d) for different welding types and value ranges of a corresponding welding gun height h are firstly provided according to work experience; and for any given new group of a welding posture included angle theta value and a welding gun displacement d value, whether the welding gun posture parameters are well selected or not is verified. The method provided by the invention has the advantages that welding technicians can be helped to rapidly evaluate whether the current set welding gun posture parameters are reasonable or not, so that a welding parameter designing method for the multi-layer welding of the butt joint of the thick-walled pipes through the TOPTIG technique is improved and optimized, the welding technique parameter designing efficiency is greatly improved, the rejection rate is reduced, and economic benefits are produced and improved.