118 results about "Botryosphaeria dothidea" patented technology

The invention discloses a burkholderia pyrrocinia JK-SH007 which is classified and named as burkholderia pyrrocinia and preserved in China center for type culture collection (CCTCC) with the preservation number of M 209028 and the preservation date of February 18th, 2009. The invention also discloses the application of the burkholderia pyrrocinia in controlling dothiorella gregaria and promoting poplar to grow. The invention further provides a bactericide for controlling dothiorella gregaria, which is obtained by inoculating the JK-SH007 in a culture medium to be fermented and cultured. The JK-SH007 is plant endophyte which has strong inhibiting effect for botryosphaeria dothidea of the poplar and antagonism for various pathogenic fungis, and has the advantages of simple culture condition, easy storage and commercial process as well as good developing and applying prospect.

The invention relates to application and preparation of bacillus amyloliquefaciens subsp. plantarum and a bacterial agent thereof. An XH-9 bacterial strain is identified as bacillus amyloliquefaciens subsp. plantarum according to mycelial morphology, colony characteristics, physiological and biochemical indexes and 16S rDNA sequence analysis and is preserved in the China General Microbiological Culture Collection Center, and a preservation number is CGMCC NO.13151. The strain is high in antagonistic effect on pathogenic fungi such as fusarium oxysporum, bipolaris sorokiniana, fusarium pseudograminearum, colletotrichum gloeosporioides, botryosphaeria dothidea and alternaria alternata and is capable of generating heteroauxin and 1-aminocyclopropanecarboxylic acid (ACC) deaminase to stimulate plant growth and capable of degrading celluloses and can be stably colonized at rhizospheres of crops such as wheat, corn, peppers and the like. The bacterial agent prepared from the bacterial strain can be applied to prevention and treatment of root rot diseases or other soil-borne fungal diseases of crops such as wheat, corn, peppers and the like and has dual functions of disease prevention and growth promotion. The bacterial agent is simple in preparation process, short in fermentation period, low in cost and beneficial to industrial production and transport.

The invention discloses a bacillus firmus strain. The bacillus firmus strain is bacillus firmus Bf-02 CGMCC No.3129, and can prevent and control a plurality of plant diseases, such as the anthracnose of walnuts, the gray mold of cucumber, wheat sharp eyespots, the ring rot of apples, tobacco brown spots, corn southern leaf blight, Dongzao jujube black patch, cotton fusarium wilt, cotton red rot, wheat scab and the like. Tests prove that the zymotic fluid of the bacillus firmus Bf-02 CGMCC No.3129 has remarkable effect of preventing and controlling vegetables, particularly the gray mold of the cucumber with the healthy growth of the vegetables and without hazards, and also remarkable growth promotion and yield increasing effects on the cucumber with the growth rate of 29.95 percent after one-time application.

The invention relates to bacillus velezensis having various disease preventive effects, application thereof and a biocontrol inoculant and belongs to the technical field of plant disease and pest prevention and control. Bacillus velezensis MS012 having bacteriostatic activity is obtained by separating and screening from healthy branches of malus sieversii. Bacillus amyloliquefaciens has remarkableantagonistic effect on botryosphaeria dothidea, colletotrichum gloeosporioides, phomopsis mali, rhizoctonia cerealis, gaeumannomyces graminis, botrytis cinerea, fusarium graminerum, clivia stem basaldecay pathogen, nandina anthrax pathogen and erwinia amylovora and has broad spectra in bacteriostatic object, and a new microbial resource is provided for biocontrol on disease and pest of plants like apple trees.

The invention discloses a panax notoginseng antimicrobial peptide gene PnSN1. The nucleotide sequence of the PnSN1 gene is shown as SEQ ID NO:1, and the PnSN1 gene is used for coding snakin antimicrobial peptide. The functional genomics correlation technique study proves that the PnSN1 gene has the function of improving the plant fungal infection resistance capability; the antifungal PnSN1 gene is built onto a plant expression vector and is transferred into tobaccos for overexpression; the transgenosis tobacco has high in vitro antifungal activity. The transgenosis tobacco subjected to PnSN1 overexpression has the strong inhibiting effect on the growth of fusarium oxysporum, fusarium solani, verticillium fusarium, botryosphaeria dothidea and beaded gibberella.

The invention discloses a Lilium regale Wilson WRKY transcription factor gene LrWRKY11. The Lilium regale Wilson WRKY transcription factor gene LrWRKY11 has a nucleotide sequence shown as SEQ ID NO: 1, and encodes a protein having an amino acid sequences shown as SEQ ID NO: 2. According to the invention, genomic studies have confirmed that the LrWRKY11 gene can improve anti-fungal functions of plants. Being constructed onto a plant expression vector and transferred into tobacco so as to be subjected to overexpression, the anti-fungal LrWRKY11 gene disclosed by the invention is capable of endowing the transgenic tobacco with very strong resistance to fungal pathogens. Experimental results have shown that transgenic tobacco with overexpression of the LrWRKY11 gene has very high level of resistance to infection of Nigrospora oryzae, Fusarium verticillioides, Botryosphaeria dothidea, Fusarium solani and Alternaria panax.

The invention discloses botryosphaeria dothidea LAMP (Loop-mediated Isothermal Amplification) detection primers and application thereof. A primer composition for detecting botryosphaeria dothidea based on an LAMP technology consists of 6 specific primers, namely F3, B3, FIP, BIP, LF and LB. The invention further provides a botryosphaeria dothidea LAMP detection method. The botryosphaeria dothideaLAMP detection method comprises the following steps: 1), extracting DNA of a sample to be detected; 2), performing loop-mediated isothermal amplification on the DNA of the sample to be detected by using the primer composition provided by the invention; 3), performing result detection, wherein when a chromogenic result is green fluorescence or a trapezoidal electrophoretic band appears, a positiveresult is determined, and when a chromogenic result is orange or orange yellow or no trapezoidal electrophoretic band appears, a negative result is determined. A novel molecular detection method and the novel primer composition are provided for the botryosphaeria dothidea, the botryosphaeria dothidea can be detected quickly, conveniently and efficiently at high specificity and high sensitivity, and a reliable technological and theoretical basis is provided for early diagnosis, detection and rapid import and export inspection of the botryosphaeria dothidea.

The invention discloses a lilium regale WRKY transcription factor gene LrWRKY3 and a preparation method thereof. A nucleotide sequence of the gene is shown as SEQ ID NO: 1, and a protein with an aminoacid sequence shown as shown in SEQ ID NO: 2; according to the invention, functional genomics related technical researches prove that the LrWRKY3 gene has the function of improving the antifungal performance of plants; the antifungal LrWRKY3 gene disclosed by the invention is constructed on a plant expression vector and is transferred into tobacco for overexpression; the transgenic tobacco plantshave very strong fungus infection resistance, and experimental results show that transgenic tobacco leaves with over-expression of LrWRKY3 have very strong resistance to infection of five pathogenicfungi such as alternaria oryzae, Botryosphaeria dothidea, fusarium graminearum, Fusarium rotunda and fusarium solani.

The invention discloses a lilium regale gene Lr14-3-3 with antifungal activity. The gene Lr14-3-3 has the nucleotide sequence as shown in SEQ ID NO:1 and encodes protein 14-3-3. A relevant technology of functional genomics proves that the gene Lr14-3-3 has a function of improving the plant antifungal activity. The antifungal gene Lr14-3-3 is constructed to a plant expression vector and is transferred into tobacco to perform overexpression; the transgenic tobacco plant has strong in vitro antifungal activity; and the transgenic tobacco expressing the Lr14-3-3 has obvious inhibition effects on the growth of botryosphaeria dothidea, phomopsis fungi, fusarium oxysporum and alternaria.

The invention discloses an organic fertilizer used for controlling hickory canker disease. The organic fertilizer is prepared from the raw materials of, by weight, 40-60 parts of traditional Chinese medicine dreg, 50-70 parts of crop straws, 0.5-1.5 parts of a fertilizer synergist, 3-7 parts of urea, 0.5-2 parts of a straw fermentation inoculant, 1-2 parts of cellulase, and 1-2 parts of brown sugar. The invention also provides a preparation method of the organic fertilizer used for controlling hickory canker disease. With the organic fertilizer, botryosphaeria dothidea activity can be substantially reduced, and hickory tree canker resistance can be enhanced. The organic fertilizer also contains organic matters, nitrogen, phosphorous, potassium and various medium and trace elements necessary for plant growth. The nutrition is comprehensive and balanced. With the organic fertilizer, soil physiochemical properties can be substantially improved, plant root system growth can be promoted, soil microbial activity can be enhanced, and soil harmful substance pollution can be reduced.

The invention discloses a specific primer used for rapid detection of a kiwi fruit soft rot pathogenic bacterium, and a detection method of the kiwi fruit soft rot pathogenic bacteriaum. A problem that existing technology is incapable of realizing accurate and rapid detection of the kiwi fruit soft rot pathogenic bacterium is solved. The specific primer comprises BZY-1 and BZY-2, the sequence of BZY-1 is 5'-CCATCAAACTCCAGTCAGTAAACG-3', and the sequence of BZY-2 is 5'-CTGCGCTCCGAAGCGAGATGTATG-3. The detection method of the kiwi fruit soft rot pathogenic bacterium comprises following steps: (1) DNA of samples are extracted; (2) the sample DNA is taken as a template, and primer BZY-1, primer BZY-2, 2*TaqPCRMasterMix and sterile ultrapure water are added for uniform mixing, and then PCR amplification is performed; and (3) an amplification product is subjected to agarose gel electrophoresis analysis, and the existence of the kiwi fruit soft rot pathogenic bacterium is determined based on the existence of amplification bands. Specificity of the primer is extremely high, only the kiwi fruit soft rot pathogenic bacterium band can be obtained by amplification, so that it is just needed to extract genome DNA from kiwi fruit tissue to be detected for PCR amplification, and the existence of the kiwi fruit soft rot pathogenic bacterium is determined based on the existence of amplification bands in electrophoretograms.

The invention provides actinomycetes which is streptomyces samsunensis XJC-2-6, and the preservation number is CCTCC NO:M 2017620. The streptomyces samsunensis XJC-2-6 provided by the invention has anantagonism on fusarium oxysporum f.sp cubense No. 4, curvularia fallax, curvularia lunata, btoryosphaeria dothidea, colletotrichum acutatum, colletotrichum gloeosporioides, colletotrichum fragariae,botrytis cinerea, fusarium oxysporum (schl.) f.sp cucumerinum owen, colletotrichum acutatum, pyricularia oryae cav, fusahum graminearum sehw, botryosphaeria dothidea and the like, has wide developmentspace on prevention and treatment of Panama disease of banana, microscler streak disease of banana, large grey speck disease of banana, tree canker of banana, anthracnose of pepper, anthracnose caused by colletotrichum gloeosporioides, anthracnose of strawberry, disease caused by botrytis cinerea, fusarium wilt of cucumber, anthracnose of mango, rice blast, wheat scab, ring rot of apple and the like, and has very good development and application prospects.

The invention discloses a lilium regale germin-like protein gene LrGLP2 with antifungal activity. The gene LrGLP2 has the nucleotide sequence as shown in SEQ ID:1 and encodes germin-like protein. A relevant technology of functional genomics proves that the gene LrGLP2 has a function of improving the plant antifungal activity. The antifungal gene LrGLP2 is constructed to a plant expression vector and is transferred into tobacco to perform overexpression; and the transgenic tobacco plant has strong in vitro antifungal activity. The LrGLP2 transgenic tobacco protein has different degrees of inhibition effects on the growth of botryosphaeria dothidea, phomopsis fungi, fusarium oxysporum and botrytis cinerea.

The invention relates to a 1,3,4-oxadiazole thioether compound as well as a preparation method and application thereof. The compound has a structure shown as a general formula (I) in the description.The compound has a good inhibition effect on pathogenic bacteria and fungi. By aiming at xanthomonas oryzae pv.oryzae, ralstonia solanacearum, cucumber xanthomonas oryzae, konjac xanthomonas oryzae, xanthomonas axonopodis pv.citri, botryosphaeria dothidea, bacterial canker and wilt of tomato, pseudomonas syringae pv.actinidae, Leucostoma cincta(Fr.:Fr.)Hohn, B.cinerea, F.oxysporum, S.sclerotiorum,G.zeae, P.infestans, P.cinnamomi and the like, good inhibition effects are achieved.

The invention relates to a botryosphaeria dothidea detection method, and in particular relates to a triplex real-time fluorescent polymerase chain reaction (PCR) detection method for botryosphaeria dothidea, belonging to the technical field of inspection and quarantine. According to the method disclosed by the invention, three groups of botryosphaeria dothidea specific primers and probes are designed and a reaction system is optimized, so that a triplex real-time fluorescent quantitative PCR detection method is established, and the detection method can be used for simultaneously detecting and identifying multiple pathogenic fungi and determining a composite invading pathogen. By applying nano magnetic microparticles to the extraction of fungal nucleic acid, the fungal nucleic acid extraction is simpler and more efficient in operation; and meanwhile, in combination with a real-time fluorescent detection technology, the detection method is applicable to the monitoring of disease occurrence in fields as well as the rapid and high-throughput detection and identification of pathogenic bacteria.

The invention discloses dual PCR primer for detecting two types of pathogens of grape canker at the same time and application of the dual PCR primer. The dual PCR primer is composed of nucleotides shown in the first sequence in the sequence list, nucleotides shown in the second sequence in the sequence list, nucleotides shown in the third sequence in the sequence list and nucleotides shown in the fourth sequence in the sequence list. The primer can detect existence of the two types of pathogens of the grape canker, namely, Neofusicoccum parvum and Botryosphaeria dothidea in a dual PCR reaction, it shows that the primer can rapidly, accurately and flexibly determine the two types of pathogens of the grape canker in grape nursery stock and field grape disease samples, and foundation is provided for nursery stock bacterium-carrying detection and specially preventing and controlling the grape canker caused by the two types of pathogens.

The invention discloses an endophytic fungus strain, which is capable of producing paclitaxel and is separated from young stems of taxus chinensis. The endophytic fungus strain is named as Botryosphaeria dothidea J11, is preserved in the China center for type culture collection and has an accession number of CCTCC NO: M2013591. The invention also relates to a method for producing paclitaxel by the preserved endophytic fungus strain. The method comprises the following steps of culturing the preserved endophytic fungus strain by a culture medium so that paclitaxel is produced and gathered in strain hyphae, recovering the paclitaxel in the cells and carrying out purification. The endophytic fungus strain provides a novel strain for microbial fermentation production of paclitaxel. Through the endophytic fungus strain, a paclitaxel fermentation production period is short and a cost is low. A HPLC analysis detection result shows that through the shake flask fermentation of the endophytic fungus strain, a paclitaxel yield has 617.6 microgrammes per liter. The endophytic fungus strain has a potential for use in industrial production.

The invention discloses Streptomyces sp.B5017. The Streptomyces sp.B5017 is characterized in that the preservation number is CCTCC NO: M2019648, and the Streptomyces sp.B5017 is classified and named as Streptomyces sp.B5017. Tests show that the marine-derived streptomyces new strain disclosed by the invention has the effect of antagonizing various phytopathogens, and has an inhibiting effect on gloeosporium musarum, colletotrichum gloeosporioides, colletotrichum pearl plum, botryosphaeria dothidea, fusarium oxysporum f.sp.cubense race 4, fusarium oxysporum f.sp.cubense race 1 microspecies or alternaria alternata. Fermentation liquor prepared from the Streptomyces sp.B5017 disclosed by the invention can effectively avoid withered and yellow withering of leaves caused by the fusarium oxysporum f.sp.cubense race 4 and race 1 in potting experiments, which proves that the Streptomyces sp.B5017 is capable of effectively preventing and treating banana wilt and enhancing the disease resistanceof bananas, has good ecological benefits and social benefits, and has a good application prospect when being developed as a biocontrol agent.

The invention relates to a bacillus subtilis strain WB1. Fermentation products, obtained through fermentation cultivation, of the strain can be used for preventing various forest-crop diseases such as botryosphaeria dothidea, valsa sp., B.dothidea and B.dothidea. The strain is preserved in the general microbiology center of the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.8439. The acquisition of the strain is hopeful to provide a safe, environment-friendly and effective approach for the prevention of the botryosphaeria dothidea and the like, and the application prospect is good.

The invention discloses a pseudo-ginseng Dirigent-like protein gene PnDIR1 and application thereof. The research by correlation techniques of molecular biology and functional genomics proves that the PnDIR1 gene has the antifungal disease function of plants. When the antifungal gene PnDIR1 is constructed onto a plant expression vector and transformed into the tobacco to be overexpressed, the transgenic tobacco plant has very high in-vitro antifungal activity; and the PnDIR1-overexpressed transgenic tobacco has obvious inhibiting actions on growth of Botryosphaeria dothidea, Fusarium solani and Verticillium Fusarium.

The invention discloses a glutathione S-transferase gene LrGSTU5, wherein the nucleotide sequence of the gene is shown as SEQ ID NO: 1; and the gene encodes the glutathione S-transferase. According to the invention, related technology of functional genomics is adopted to prove that the lilium regale LrGSTU5 gene has the function of improving the antifungal ability of plants; the lilium regale glutathione S-transferase gene LrGSTU5 provided by the invention is built on plant expression carriers and then transferred into tobacco for overexpression; the transgenic tobacco has a high in-vitro antifungal activity; and the transgenic tobacco protein expressing LrGSTU5 has an obvious inhibition effect on the growth of various pathogenic fungi such as botryosphaeria dothidea, fusarium oxysporum, alternaria sp and the like.

The invention provides trichoderma asperellum TZ312, application thereof, a volatile metabolite thereof and application of the volatile metabolite, and relates to the technical field of microorganisms. The trichoderma asperellum TZ312 is preserved in Guangdong Microbiological Culture Collection Center and has a preservation number of GDMCC NO:60253. The trichoderma asperellum TZ312 is capable of completely inhibiting and covering growth of hyphae of banana wilt disease germs on a panel; and the trichoderma asperellum TZ312 is capable of obviously decreasing the occurrence rate of a banana wiltdisease in a greenhouse pot experiment, and the prevention effect is 65% or above. The trichoderma asperellum TZ312 is further capable of inhibiting fusarium oxysporum f.sp.cucumerinum, botrytis cinerea, botrytis cinerea, fusarium solani, fusarium proliferatum, magnaporthe oryzae, phomopsis sp., pestalotiopsis sp., botryosphaeria dothidea or colletotrichum gloeosporioides and is wide in antibacterial spectrum. The volatile metabolite of the trichoderma asperellum TZ312 can be used for inhibiting preservation diseases.

The invention discloses a mycorrhiza helper bacterium Bacillus spp and its application in populus growth promotion. The above mycorrhiza helper bacterium is preserved in the China Center for Type Culture Collection in Wuhan University in Wuhan, China on April 6, 2013 as Bacillus spp. MY-DZ18, and has a preservation number of CCTCC NO:M2013125. The Bacillus spp. MY-DZ18 is a mycorrhiza helper bacterium, can promote the mycelia growth of two ectomycorrhizal fungi comprising Pisolithus tinctorius and Lactarius insulsus; pot experiment results show that the respective double inoculation of the MY-DZ18 strain with the two ectomycorrhizal fungi substantially improves the mycorrhiza formation rate of the root of the populus, and promotes the growth of the populus; and the MY-DZ18 strain has an antagonistic effect on Botryosphaeria dothidea Phomopsis sp., Cytospora chrysosperma and seedling damping-off pathogen Fusarium oxysporum.

The invention relates to an antagonistic strain which is finally identified as Bacillus amyloliquefaciens through physiological and biochemical reaction, 16SrDNA and gyrA gene sequencing and sequence alignment; the Bacillus amyloliquefaciens is named Bacillus amyloliquefaciens YTB1407. The Bacillus amyloliquefaciens strain has antagonistic activity to various fruit tree pathogenic fungi, including macrophoma kawatsukai, ahernaria mali, grape anthracnose, coniothyrium diplodiella, plasmopara viticola and the like; the Bacillus amyloliquefaciens strain is antibacterial in broad spectrum, stable in anti-microbial activity and lasting in effect and has the huge potential for developing novel, efficient and natural biological control agents.

The invention belongs to the field of agricultural bactericides and herbicides, and particularly discloses triazole Mannich base compounds containing trifluoromethyl and piperazine, as well as preparation methods and application of the triazole Mannich base compounds. The triazole Mannich base compounds have structural formulas shown in formula I and formula II; in the structural formulas, R1 and R2 are defined as claim 1. The triazole Mannich base compounds have certain in-vitro inhibitory activities on such plant pathogens as cucumber fusarium wilt pathogens, peanut cercospora brown spot pathogens, apple ring spot pathogens, tomato early blight pathogens, wheat sharp eyespot pathogens and wheat scab pathogens, and particularly has relatively high in-vitro inhibitory activities on the apple ring spot pathogens and the wheat sharp eyespot pathogens. The triazole Mannich base compounds with the structural formulas shown in formula I and formula II both have certain weeding activities on oilseed rapes, and have relatively high in-vitro inhibitory activities on ketol-acid reductoisome-rase (KARI) of rice. The triazole Mannich base compounds are applicable to comprehensive control of the damage caused by bacteria and weeds, and inhibition on the KARI activity.

The invention relates to the technical field of functional microorganism screening and application, in particular to novel bacillus mucilaginosus and an application thereof in agricultural production.The preservation number of the bacillus mucilaginosus is CCTCC NO: M2020163. The bacillus mucilaginosus can effectively improve the soil fertility, promote crop growth and increase the yield, has a very good prevention and treatment effect on plant diseases such as apple ring rot, brown spot and cucumber root-knot nematode disease, and has a wide application prospect.

The invention provides a microbial agent for preventing and treating apple ring rot and a preparation method thereof. The microbial agent is obtained by using a gel carrier loaded with compound microbial powder. The compound microbial agent comprises bacillus cereus ACCC03288 powder, trichoderma harzianum ACCC30371 powder, pseudomonas chlororaphis ACCC19853 powder, paecilomyces lilacinus ACCC30639powder and thorn spore streptomyces hygroscopicus ACCC40018 powder. The growth and reproduction of apple ring rot germs are synergistically inhibited, nutrition support is provided for apple trees, and the growth of apples is promoted. The gel carrier is obtained by utilizing a cross-linking reaction of acrylamide and sodium acrylate, cross-linking sodium alginate and calcium chloride and introducing polyvinyl alcohol, can be naturally degraded, has a slow release effect on the compound microbial powder, and provides nutrition support for the microbial growth, so that the compound microbial powder has a better control effect. The microbial agent only needs to be applied once, thereby reducing the labor intensity.

The invention provides a preparation method of penicillium oxalicum. The preparation method comprises the steps: seed culture, amplification culture, liquid membrane extraction and bactericide preparation. The penicillium oxalicum is capable of preventing and controlling various fruit and vegetable diseases by single strain, wide in antibacterial spectrum and capable of effectively reducing the preventing and controlling costs; and the bacterium inhibiting rate for various fruit and vegetable diseases such as kiwifruit bacterial canker, bacterial leaf shot hole, apple ring rot, potato dry rotand peach brown rot reaches up to 90% or above.

The invention discloses notoginseng pathogenesis-related protein gene PnPRlike and application. The nucleotide sequence of the PnPRlike gene is shown in SEQ ID NO: 1, which is an encoding pathogenesis-related protein; the related technical research of functional genomics proves that the PnPRlike gene has the function of improving the resistance of plants to pathogenic fungus, and the antifungal gene PnPRlike is constructed onto a plant expression vector and is converted into tobacco for overexpression, transgenic tobacco plant has extremely high in vitro anti-fungal activity, and experimentalresults show that the over-expression transgenic tobacco for PnPRlike has an obvious inhibiting effect for the growth of Fusarium solani, Botryosphaeria dothidea and Nigrospora oryzae.

The invention discloses a specific primer and a variation interval, wherein the specific primer is used for rapidly detecting pathogenic bacterium lasiodiplodia theobromae causing various plant diseases, and the variation interval is used for designing the specific primer. The variation interval for designing an L.theobromae specific primer is discovered according to transcription elongation factor (TEF) sequences of botryosphaeria dothidea fungi and other fungi comprising the lasiodiplodia theobromae in a GenBank, a high-specificity primer pair ZYLTF1 / ZYLTR1 is designed based on an interval sequence and can specifically amplify a 216bp stripe only from L.theobromae, and any stripes can not be amplified from other species. The invention further provides a rapid detecting method of the primer pair for the lasiodiplodia theobromae. The rapid detecting method has the advantages of good specificity, high sensitivity, economy, rapidness and the like, the method can rapidly and accurately detect whether the L.theobromae exists or not, and the method can play a pivotal role in early prevention and control of plant diseases.