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Mycorrhiza helper bacterium Bacillus spp and its application in populus growth promotion

A technology of bacterial spores and bacillus, applied in application, bacteria, plant growth regulators, etc., can solve problems such as large nutrient consumption, decline in soil fertility, and decline in forest productivity.

Active Publication Date: 2013-09-25
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because poplar consumes a lot of nutrients, it is easy to cause soil fertility to decline, so fertilization has become an indispensable technical measure for poplar production.
However, with the increase in the use of chemical fertilizers, the negative impacts of environmental pollution, forestland productivity decline, and soil biodiversity reduction are becoming more and more obvious.

Method used

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  • Mycorrhiza helper bacterium Bacillus spp and its application in populus growth promotion
  • Mycorrhiza helper bacterium Bacillus spp and its application in populus growth promotion
  • Mycorrhiza helper bacterium Bacillus spp and its application in populus growth promotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Growth-promoting effect of Bacillus MY-DZ18 on colored soybean puffball (Pt2) and bad-tasting milk mushroom (Li)

[0023] After activating the test bacterium MY-DZ18 isolated from the soil sample, a small amount was inoculated into a Erlenmeyer flask containing 50 mL of TSA liquid medium with an inoculation loop, and cultured with shaking at 28 °C and 160 r / min for 72 h. Centrifuge at 4°C and 5000r / min for 5min, discard the supernatant after centrifugation, and obtain bacterial cells. Rinse the bacteria twice with normal saline, and finally make a bacterial suspension with normal saline, which is the bacterial inoculum.

[0024] (1) Determination of the growth-promoting effect of Bacillus MY-DZ18 suspension on colored bean puffballs and bad-tasting milk mushrooms.

[0025] The test was carried out by the dry dish resistance method. Cultivate colored bean puffballs and bad-tasting milk mushrooms on the MMN medium. When the mycorrhizal fungi grow to the vigoro...

Embodiment 2

[0034] Example 2 The growth-promoting effect of Bacillus MY-DZ18 interacting with colored bean puffballs and milk mushrooms with bad taste on poplar.

[0035] Poplar seedlings were used as test plants. Poplar seeds were collected from Siyang, Jiangsu in June 2011, and the parent tree species was Populus deltoides. Put the soil matrix into pots after being sterilized by high temperature and damp heat method, and the poplar seeds are surface-sterilized with 1‰ concentration of potassium permanganate and then sowed into the pots, with 3 to 4 seeds per pot. When the poplar saplings grow to a certain height, select the one with the best growth and keep it, and remove the rest. Cultivate under natural conditions, keep the watering amount, light, ventilation, temperature and other conditions the same.

[0036] Preparation of Bacillus MY-DZ18 cell suspension: After activating each strain, use an inoculation loop to inoculate a small amount into a 100mL Erlenmeyer flask containing 50...

Embodiment 3

[0043] Embodiment 3 bacillus MY-DZ18 is to the plate bacteriostasis of three kinds of phytopathogenic fungi

[0044] The pathogenic bacteria cake was inoculated in the center of the ZPD plate, and the purified MHB strain MY-DZ18 was spotted on the four corners 25 mm away from the bacteria cake. The control plate was only inoculated with the pathogenic fungus cake, and the test was repeated 3 times. Place the finished plate in an incubator at 25°C for 3 to 5 days, and observe whether the strain has antagonism against the three pathogenic bacteria. The result is as image 3 , it can be seen from the figure that the MY-DZ18 strain has a certain inhibitory effect on the three pathogenic bacteria.

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Abstract

The invention discloses a mycorrhiza helper bacterium Bacillus spp and its application in populus growth promotion. The above mycorrhiza helper bacterium is preserved in the China Center for Type Culture Collection in Wuhan University in Wuhan, China on April 6, 2013 as Bacillus spp. MY-DZ18, and has a preservation number of CCTCC NO:M2013125. The Bacillus spp. MY-DZ18 is a mycorrhiza helper bacterium, can promote the mycelia growth of two ectomycorrhizal fungi comprising Pisolithus tinctorius and Lactarius insulsus; pot experiment results show that the respective double inoculation of the MY-DZ18 strain with the two ectomycorrhizal fungi substantially improves the mycorrhiza formation rate of the root of the populus, and promotes the growth of the populus; and the MY-DZ18 strain has an antagonistic effect on Botryosphaeria dothidea Phomopsis sp., Cytospora chrysosperma and seedling damping-off pathogen Fusarium oxysporum.

Description

technical field [0001] The invention belongs to the field of microbial fertilizers, in particular to an ectomycorrhizal auxiliary bacterium bacillus and its application in promoting the growth of poplars. Background technique [0002] Poplar (Populus) is mainly distributed in the northern hemisphere, especially in Asia and North America, and is the main tree species for afforestation in these areas. Poplar has many kinds and grows rapidly, which can solve the problem of timber shortage in a short period of time, and occupies a very important position in forestry production. However, because poplar consumes a lot of nutrients, it is easy to cause soil fertility to decline, so fertilization has become an indispensable technical measure for poplar production. However, with the increase in the use of chemical fertilizers, the negative impacts such as environmental pollution, decline in forest productivity, and reduction in soil biodiversity are becoming more and more obvious. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/07
Inventor 吴小芹赵柳叶建仁李桂娥
Owner NANJING FORESTRY UNIV
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