The invention provides a method for promoting accumulation of
triterpene in
betula platyphylla suk. Suspension
cell by utilizing endophytic fungi
elicitor. The method utilizes endophytic fungi separated from betula latyphylla suk.
bark, the endophytic fungi is prepared into fungi
elicitor, appropriate concentration of fungi
elicitor is added in appropriate stage of
betula platyphylla suk. suspension
cell growth, after induction is carried out for a period of time, cells are collected, and endophytic fungi capable of effectively improving accumulation of
triterpene is screened out as
phomopsis sp. On the basis,
induction effect of
polysaccharide elicitor and
protein elicitor prepared by the fungi is further optimized; 40Mug / ml of
polysaccharide elicitor is added on the eighth day of
betula platyphylla suk.
cell culture,
triterpene content in cell after induction for one day reaches up to 29.47mg / g, being 1.78 times that of the control group; 80Mug / ml of
fungus protein elicitor is added on the eighth day of betula platyphylla suk.
cell culture, induction culture is carried out for four days, and triterpene content in cell reaches up to 34.49mg / g, being 2.47 times that of the control group. In the invention, the type and concentration of the added elicitor, growth stage of betula platyphylla suk. suspension cell added with appropriate elicitor and
induction time of elicitor are determined, induction technology of effectively improving accumulation of triterpene in betula platyphylla suk. suspension cell is formed, and important theory and technical basis are laid for betula platyphylla suk. triterpene production by utilizing
cell engineering technology, thus the invention has wide application potential and promotion prospect.