Phomopsis fungus strain E41 and applications thereof
A technology of phosphimase and fungus, which is applied to the fungal strain E41 of the genus Phomopsis and its application field in degrading the herbicide dimethyltetrachloride, and can solve the problem that pregnant women are prone to miscarriage, the content exceeds a safe value, and the natural environment is damaged. and other issues, to achieve the effect of important industrial application value, good market prospects and easy industrialization
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Embodiment 1
[0020] Example 1 : Isolation, screening and identification of Phomopsis sp. E41
[0021] 1.1 Experimental materials
[0022] Inorganic salt medium: 1 g of ammonium nitrate, 1.5 g of dipotassium hydrogen phosphate, 0.5 g of potassium dihydrogen phosphate, 0.1 g of crystalline magnesium sulfate, 45.72 mg of ferrous sulfate (7 water), 1 L of water, 40 g of agar.
[0023] LB medium: 10g tryptone, 5g yeast powder, 10g sodium chloride, 1L water, 40g agar.
[0024] PDA medium: 200g potatoes, 20g glucose, 500-600mL water, boiled for about half an hour, passed through gauze, dilute to 1 liter, add 40g agar.
[0025] The degradation solution is a mixture of inorganic salt medium and dimethyltetrachloride.
[0026] 1.2 Separation and identification methods
[0027] Phomopsis sp. E41 of the present invention is obtained from sampling, isolation and screening in Mingyang, Guangxi, and is preserved by the Guangdong Microbial Culture Collection Center with a preservation number of GDMCC...
Embodiment 2
[0041] Example 2 : Study on the effect of Phomopsis sp. E41 on the degradation of dimethyltetrachloride
[0042] 2.1 Cultivation of degrading bacteria
[0043] Prepare PDB medium, sterilize it, and put it in sterilized 30mL vials with caps, 10mL per bottle, scrape the purified fungal hyphae in PDA medium with an inoculation loop, and insert them into PDA medium vials respectively, To obtain expanded cultured cells, the culture condition is to cultivate at 28°C for 3-4 days and wait for use.
[0044] 2.2 Screening of degrading bacteria
[0045] Prepare the degradation test medium of degrading bacteria, and distribute it in sterile 30mL vials with caps, 10mL per bottle. Add 0.55g / L (by dry weight) to expand the cultured cells and mix with the subpackaged degrading bacteria degradation test medium. Before mixing, wash with the degradation bacteria degradation test medium for 3 times and then mix with the degradation test medium , three parallel treatments and a blank control...
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