43 results about "Pestalotiopsis sp." patented technology

The invention discloses a method for manually inducing Aquilaria sinensis to generate agilawood. The method comprises the following steps of: injecting a methanoic acid or acetic acid solution with pH value of 1.5-3 or a mixed solution of methanoic acid and acetic acid into xylem of Aquilaria sinensis [Aquilaria sinensis (Lour.) Gilg]; respectively injecting the solution together with fungi, suchas Botryosphaeria rhodina, Hypocrea jecorina, Fusarium sp., Colletotrichum gloeosporioides, Ampelomyces sp, Khuskia sp, Fungal endophyte sp.A 16, Pestalotiopsis sp., Hypocrea lixii and Chaetomium sp.or injecting the solution and the fungi in a mixed way; and inducing the Aquilaria sinensis to generate the agilawood. According to the invention, the methanoic acid or acetic acid solution or the mixed solution of methanoic acid and acetic acid is injected into the xylem of the Aquilaria sinensis to induce the Aquilaria sinensis to generate defense reaction so as to generate the agilawood; and alarge amount of agilawood are generated by using the method. The method for manually inducing the Aquilaria sinensis to generate the agilawood, disclosed by the invention, has the advantages of simplicity and convenience in operation, low cost and suitability for manually making agilawood on a large scale by using Aquilaria sinensis trees; and a large amount of agilawood can be effectively generated by the Aquilaria sinensis. According to the invention, the agilawood producing period of the Aquilaria sinensis is largely reduced, the agilawood can be generally obtained after 6-24 months, the production cost is reduced, the market requirements are satisfied, and important economic, social and ecological benefits are obtained in aspects of protection and sustainable utilization of the Aquilaria sinensis, economic development of mountainous regions and the like.

The invention provides streptomycete for producing volatile antibacterial substances and application thereof. The streptomycete is named as Streptomycesmicionensis TF78; the streptomycete was collected to Guangdong Microbiological Culture Collection Center on October 25th, 2017, and the collection number is GDMCC NO:60254. The streptomycete has the inhibiting functions on the pathogenic bacteria of cucumber blight, tomato gray mold, teak canker, banana fungal stem rot, rice blast fungi, mango phomopsis leaf spot, mango pestalotiopsis leaf spot, mango botryosphaeria branch blight, mango anthracnose and the like.

The invention discloses a paenibacillus polymyxa bacterial strain and an application thereof. The paenibacillus polymyxa bacterial strain is PBSZ123, the preservation name is paenibacillus polymyxa (paenibacillus polymyxa), the paenibacillus polymyxa bacterial strain is preserved in China General Microbiological Culture Collection Center, CGMCC, the preservation work address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is August 14, 2019, and the preservation No. is CGMCC No.18386. The paenibacillus polymyxa is obtained by separation from parispolyphyllavar.yunnanensis (Franch.)Hand endophyte, and has favorable inhibition effects on main pathogenic bacteria of parispolyphyllavar.yunnanensis (Franch.)Hand endophyte diseases of botrytis cinerea, phoma sp. and pestalotiopsis sp.

The invention discloses dendrobium officinale Kimura et Migo endophytic fungus. The dendrobium officinale Kimura et Migo endophytic fungus is obtained by separating from the living body of dendrobium officinale Kimura et Migo belonging to dendrobium species, orchidaceae by adopting an endophytic fungus separation and purification technology; and identified by the microbial taxonomy, the dendrobium officinale Kimura et Migo endophytic fungus is Pestalotiopsis sp.DO14; the strain is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number CCTCC M 2015180. Through co-culture with dendrobium officinale Kimura et Migo tissue culture seedlings, the steam thickening and lengthening, and the stem and leaf color deepening of the dendrobium officinale Kimura et Migo tissue culture seedlings can be promoted, the improvement of the polysaccharide content in the stem can be promoted, the change of alcohol soluble ingredients in the stem and the flavonoid ingredients in the leaves can be promoted; the dendrobium officinale Kimura et Migo endophytic fungus is an important microbe closely related to the growth and metabolites of dendrobium officinale Kimura et Migo, and has the application potential of promoting the growth of dendrobium officinale Kimura et Migo and the change of the metabolic components.

The invention discloses a liquid fermentation method for producing laccase by using marine endogenous pestalotia, which comprises the following steps of: (1) screening a strain: screening soil in the depth of 10-20m on the offshore of the East China Sea to obtain a novel marine fungi; (2) preserving the strain: inoculating the strain in a modified potato dextrose agar (PDA) medium for culture, and preserving on a slope at the temperature of 4DEG C; (3) activating and propagating the strain: inoculating a spore of the strain in a PDA medium added with special components, and inoculating the activated spore in a seed culture medium for propagation; (4) preparing a marine endogenous pestalotia liquid fermentation medium; and (5) fermenting: performing liquid fermentation and enzyme production by using the newly prepared medium, and determining the optimized enzyme production condition. In the method for producing the laccase, the novel marine endogenous pestalotia is taken as a production strain; and the method has the characteristic that the media are coarse, wide in sources, low in prices and the like.

The invention discloses an alkaloid dimer, a preparation method thereof and application of the alkaloid dimer as an antiviral agent. The method comprises the following steps: firstly, carrying out strain culture on fungus Pestalotiopsis sp.(ZJ-2009-7-6) during preparation, and carrying out fermental cultivation on the fungus; leaching the obtained mycelium with a chloroform-methanol mixed liquid (1 to 1) for three times, carrying out vacuum concentration, and extracting with ethyl acetate for three times, so as to obtain crude extractum; and sequentially carrying out positive slica column chromatography, Sephadex LH-20 gel column chromatography and HPLC (high-performance liquid chromatography) on ethyl acetate-phase crude extractum to obtain a compound shown in a formula I. The antiviral agent provided by the method has the characteristics that the compound shown in the formula I or a pharmaceutically acceptable salt is taken as an effective component for preventing and / or treating diseases caused by herpes simplex virus I (HSV-1) and / or enterovirus 71 (EV71).

The invention relates to the discovery of a compound with strong inhibitory activity on the growth of fusarium moniliforme and a preparation method, which belong to the technical field of biological pesticide. The compound is obtained through production strain smoky pestalotiopsis sp SN17 by adopting a solid fermentation and extraction separation method, and the structure of the compound is determined through using the technologies such as mass spectrum, nuclear magnetic resonance spectrum, infrared spectrum and the like. The compound of the invention is restalachloride A, which can effectively inhabit the growth of the fusarium moniliforme and can be singly used as the natural antagonist of the fusarium moniliforme.

The invention discloses muscodor sp.W-S-41, the preservation number of which is CCTCC NO: M2016759. The muscodor sp.W-S-41 can inhibit plant pathogenic fungi comprising Botrytis cinerea ZAU10, Pestalotiopsis sp. ZAU20, Fusarium sp. ZAU 28, Penicillium digitatum KH08, Pythium ultimum ZAU22 and the like.

The invention discloses a chloro benzophenone compound which is prepared by the following steps: firstly carrying out strain culture of Pestalotiopsis sp.(HM486429) in a strain culture medium; secondly, carrying out fermentation culture of the fungus in a fermentation culture medium, then extracting the obtained mycelium three times by using ethyl acetate and extracting the obtained mycelium three times by using a chloroform-methanol mixed solution (1:1), carrying out vacuum concentration so as to obtain a crude extract, carrying out chromatographic separation, and respectively concentrating the eluant so as to obtain white powder, which is pestalachloride D, wherein the structural identification is shown in the specification. The invention provides an antibacterial agent which has optical inhibitory activities for Vibrio parahaemolyticus, Vibrio anguillarum and Escherichia coli, can be used for developing optical antibacterial agents, and is wide in application prospect as the raw materials can be produced in a large scale through fungus fermentation and no resource limit exists.

The invention provides trichoderma asperellum TZ312, application thereof, a volatile metabolite thereof and application of the volatile metabolite, and relates to the technical field of microorganisms. The trichoderma asperellum TZ312 is preserved in Guangdong Microbiological Culture Collection Center and has a preservation number of GDMCC NO:60253. The trichoderma asperellum TZ312 is capable of completely inhibiting and covering growth of hyphae of banana wilt disease germs on a panel; and the trichoderma asperellum TZ312 is capable of obviously decreasing the occurrence rate of a banana wiltdisease in a greenhouse pot experiment, and the prevention effect is 65% or above. The trichoderma asperellum TZ312 is further capable of inhibiting fusarium oxysporum f.sp.cucumerinum, botrytis cinerea, botrytis cinerea, fusarium solani, fusarium proliferatum, magnaporthe oryzae, phomopsis sp., pestalotiopsis sp., botryosphaeria dothidea or colletotrichum gloeosporioides and is wide in antibacterial spectrum. The volatile metabolite of the trichoderma asperellum TZ312 can be used for inhibiting preservation diseases.

The invention discloses a penicillium brefeldianum strain and application thereof. The penicillium brefeldianum strain is PFLJ04, the preservation name of the strain is penicillium brefeldianum, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, and the preservation date is August 15, 2019; and the preservation number is CGMCC No. 18119. Penicillium is separated from paris polyphylla var. yunnanensis endophyte, and has a good inhibiting effect on panax notoginseng root rot fungi (fusarium oxysporum, fusarium solani and phytophthoracactorum), paris polyphylla leaf spot fungi and paris polyphylla botrytis cinerea (botrytis cinerea, phoma sp. and pestalotiopsis sp.) simultaneously.

The invention discloses an alkaloid compound as well as a preparation method and an application of the alkaloid compound. The alkaloid compound has a molecular formula of C15H21NO4. The preparation method of the alkaloid compound comprises the steps that activated Pestalotiopsis sp. fungi Z233 are inoculated into culture liquid, and the fermentation culture is carried out for 10 to 40 days at 20 to 30 DEG C; mycelia and fermentation liquid are obtained through separation after the fermentation culture; after the mycelia are leached, extracted and concentrated by organic solvents, mycelium extractives are obtained; or the fermentation liquid is extracted and concentrated to obtain fermentation liquid extractives; and the chromatographic separation and the purification treatment are carried out on the mycelium extractives or the fermentation liquid extractives, and the alkaloid compound is prepared. The alkaloid compound with a novel structure is extracted and separated from fermentation culture substances of Sargassum horneri epiphytic Pestalotiopsis sp. fungi Z233, and the alkaloid compound has better antitumor activity, can be used for preparing antitumor medicine and tumor prevention health-care food and has good development prospects.

The invention provides streptomyces murinus and application thereof. The streptomyces murinus is named streptomyces murinus FXQ-5B, is preserved in the China Center for Type Culture Collection on October 16, 2014, and has a preservation number of CCTCC M2014487. The streptomyces murinus FXQ-5B can be used for effectively inhibiting growth and spore germination of camellia oleifera leaf blight pathogen, has the advantages of remarkable prevention effect, no pollution and good environment safety, and has an excellent effect for inhibiting other bacteria, such as fusarium oxysporum, lasiodiplodia theobromae, diaporthe phaseolorum, bipolaris setariae, pestalotiopsis menezesiana and the like. The streptomyces murinus has broad-spectrum anti-disease, and has an important and profound significance for prevention and treatment of camellia oleifera and other diseases.

The invention discloses a method for manually inducing Aquilaria sinensis to generate agilawood. The method comprises the following steps of: injecting a methanoic acid or acetic acid solution with pH value of 1.5-3 or a mixed solution of methanoic acid and acetic acid into xylem of Aquilaria sinensis [Aquilaria sinensis (Lour.) Gilg]; respectively injecting the solution together with fungi, suchas Botryosphaeria rhodina, Hypocrea jecorina, Fusarium sp., Colletotrichum gloeosporioides, Ampelomyces sp, Khuskia sp, Fungal endophyte sp.A 16, Pestalotiopsis sp., Hypocrea lixii and Chaetomium sp.or injecting the solution and the fungi in a mixed way; and inducing the Aquilaria sinensis to generate the agilawood. According to the invention, the methanoic acid or acetic acid solution or the mixed solution of methanoic acid and acetic acid is injected into the xylem of the Aquilaria sinensis to induce the Aquilaria sinensis to generate defense reaction so as to generate the agilawood; and alarge amount of agilawood are generated by using the method. The method for manually inducing the Aquilaria sinensis to generate the agilawood, disclosed by the invention, has the advantages of simplicity and convenience in operation, low cost and suitability for manually making agilawood on a large scale by using Aquilaria sinensis trees; and a large amount of agilawood can be effectively generated by the Aquilaria sinensis. According to the invention, the agilawood producing period of the Aquilaria sinensis is largely reduced, the agilawood can be generally obtained after 6-24 months, the production cost is reduced, the market requirements are satisfied, and important economic, social and ecological benefits are obtained in aspects of protection and sustainable utilization of the Aquilaria sinensis, economic development of mountainous regions and the like.

The invention relates to a paenibacillus polymyxa DX32 strain. The preservation number of the paenibacillus polymyxa DX32 strain is CCTCC No: M 2020639. The invention also discloses an application of the paenibacillus polymyxa DX32 in inhibition of pathogenic fungi of a pestalotiopsis plant. The paenibacillus polymyxa DX32 disclosed by the invention can be used for effectively inhibiting pathogenic fungi of pestalotiopsis plants, such as pestalotiopsis microspora, pestalotiopsis theae, pestalotiopsis chamaeropis, pestalotiopsis versicolor and pestalotiopsis paeoniae, and has a relatively good inhibition effect on hypha and spore germination.

The invention discloses a method for extracting taxol from a Hainan Pestalotiopsis sp. F metabolite. The method comprises the steps of thallus culturing and fermentation; secondary metabolite extraction; and column chromatography purification. Taxol produced by the extracted Hainan Pestalotiopsis sp. F has a same structure with standard taxol, and the output is 1466.87[mu]g / L. The taxol output of the above fungus in panda scurf is substantially higher than that of the fungus from other animals. Production of taxol from the fungus may be a potential candidate method for industrial large-scale production of taxol.

The invention belongs to the field of the microbial technology, and discloses a pestalotipsis fungus and applications of the pestalotipsis fungus. Through the analysis on the separation and character variation of endophytic fungi of cordyceps sinensis, culture, isolation and purification are carried out on the mole cricket insect body and claws by adopting different culture media, then the pestalotipsis fungus is separated and screened, and the fungus is preserved in the Guangdong Microbiological Culture Collection Center on Dec 26, 2016, and is assigned with the accession number of GDMCC No: 60135. The fungus is in good growth conditions under the conditions that the rice culture medium is adopted, the temperature is 20 DEG C and no illumination is available, nucleoside with high content can be produced, the discovery of the fungus can realize the abundant fermentation and production of nucleoside in vitro, and thus the fungus has the wide application value.

The invention relates to the technical field of microorganisms, the artemisia japonica endophytic fungi is isolated from artemisia japonica plant living body which is collected from suburb of Guiyang City Guizhou Province by endophytic fungi separation technology, by microbial taxonomic identification, the artemisia japonica endophytic fungi is named as pestalotiopsis uvicola GMH31, the strain is preserved in China Center For Type Culture Collection (CCTCC), the preservation date is June 30, 2014, and the preservation registration number is CCTCC NO:M2014303. The most striking feature of the Pestalotiopsis uvicola GMH31 is that the fermentation liquor of the strain can produce active metabolites with inhibiting effects on kiwi fruit sclerotinia sclerotiorum, phytophthora capsici and other pathogenic fungi of plant fungal diseases, and the Pestalotiopsis uvicola is an important biological resource in agriculture.

The invention discloses a pestalotiopsis fungus chitin deacetylase and a preparation method and application thereof. According to codon preference of pichia pastoris, an optimized nucleic acid sequence is shown as SEQ ID NO.2, and a chitin deacetylase encoding gene sequence of the pestalotiopsis fungus is obtained by using a whole-gene synthesis method. Furthermore, a pichia pastoris expression system is used for carrying out secretory expression on the optimized chitin deacetylase encoding gene to obtain chitin deacetylase, and the amino acid sequence of the chitin deacetylase is shown as SEQID NO.1. The chitin deacetylase obtained by the invention can be used for removing acetyl in chitin, chitosan and chitosan oligosaccharide, so that chitosan or chitosan oligosaccharide with a specific structure is obtained. These chitosan or chitosan oligosaccharides of specific structures may have a new or higher biological activity. Therefore, the pestalotiopsis fungus chitin deacetylase and the deacetylation product thereof have good industrial application prospects.

The invention discloses application of a melatonin derivative to prevention and treatment of plant fungal diseases, and belongs to the field of pesticides. According to the present invention, the compound has characteristics of strong inhibition activity on the pathogenicity of Magnaporthe oryzae, good broad spectrum, and good inhibition effect on Fusarium graminearum, Botrytis cinerea, Sclerotinia sclerotiorum, Pestalotiopsis, Monilinia fruticosa, Bipolaris maydis, and Rhizoctonia solani, and is the potential broad spectrum pesticide antibacterial molecule, and can be used for the preparation of the antibacterial agent, such that the antibacterial agent can be used for the preparation of the antibacterial agent, and can be used for the preparation of the antibacterial agent, such that the antibacterial agent can be used for the preparation of the antibacterial agent, and the antibacterial agent can be used for the preparation of the antibacterial agent, and can be used for the preparation of the antibacterial agent, and can be used for the preparation of the antibacterial agent.

The invention discloses dendrobium officinale Kimura et Migo endophytic fungus. The dendrobium officinale Kimura et Migo endophytic fungus is obtained by separating from the living body of dendrobium officinale Kimura et Migo belonging to dendrobium species, orchidaceae by adopting an endophytic fungus separation and purification technology; and identified by the microbial taxonomy, the dendrobium officinale Kimura et Migo endophytic fungus is Pestalotiopsis sp.DO14; the strain is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number CCTCC M 2015180. Through co-culture with dendrobium officinale Kimura et Migo tissue culture seedlings, the steam thickening and lengthening, and the stem and leaf color deepening of the dendrobium officinale Kimura et Migo tissue culture seedlings can be promoted, the improvement of the polysaccharide content in the stem can be promoted, the change of alcohol soluble ingredients in the stem and the flavonoid ingredients in the leaves can be promoted; the dendrobium officinale Kimura et Migo endophytic fungus is an important microbe closely related to the growth and metabolites of dendrobium officinale Kimura et Migo, and has the application potential of promoting the growth of dendrobium officinale Kimura et Migo and the change of the metabolic components.

The invention discloses a Metschnikowia pulcherrima strain for preventing and treating grey speck disease after loquat harvest and an application thereof, which belongs to the technical field of postharvest biological prevention and treatment of fruits. The biocontrol saccharomycetes is identified as Metschnikowia pulcherrima, and a preservation number is CCTCC M 2019256. The preparation method comprises the following steps: firstly, preparing a Pestalotiopsis vismiae P2 conidiospore suspension and a Metschnikowia pulcherrima E1 bacterial suspension for later use; punching a hole (3mm*3mm) inan equator part of the loquat fruit, adding the E1 bacterial suspension, and adding P2 conidiospore suspension after 2 hours; and airing, putting the material into a plastic basket, sealing with a preservative film, and storing the material at room temperature. The morbidity and rotting direct drop of the loquat grey speck disease treated by the bacterial suspension are obviously reduced. Therefore, the Metschnikowia pulcherrima can be used for preventing and treating the grey speck disease after loquat harvest, and the harm to human health and the pollution to the environment caused by the use of chemical sterilization are reduced.

The invention discloses a bacillus polymyxa strain and application thereof. The Bacillus polymyxa strain of the present invention, the Bacillus polymyxa strain is PBSZ123, the preserved name is Bacillus polymyxa (Paenibacillus polymyxa), and it is preserved in the General Microorganism Center (CGMCC) of China Microorganism Culture Preservation Management Committee, the depository unit The address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. Date of deposit: August 14, 2019; Collection number: CGMCC No.18386. The bacillus polymyxa of the present invention is isolated from the endophyte of the polyphylla, and has a good inhibitory effect on the main pathogenic bacteria of the polyphylla, Botrytis cinerea, Phoma leaf spot, and Polychaete spp.

The invention discloses a strain of Penicillium breenii and its application. The Penicillium brefeldianum strain of the present invention, the Penicillium brefeldianum strain is PFLJ04, and the preservation name is Penicillium brefeldianum, which is preserved in the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee, and the depository address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, date of deposit: August 15, 2019; deposit number: CGMCC No.18119. The penicillium of the present invention is isolated from the endophytic bacteria of P. diangi, and can simultaneously treat notoginseng root rot pathogens (Fusarium oxysporum, Fusarium solani, Phytophthora spp.) The pathogens (Botrytis cinerea, Phoma leaf spot, and Polychaete spp.) have good inhibitory effect.

The invention relates to the technical field of microorganisms. An artemisia japonica endophytic fungus is collected from living bodies of artemisia japonica from a suburb of Guiyang city in Guiyang province through endophytic fungus separation technology. The artemisia japonica endophytic fungus is named pestalotiopsis uvicola GMH 31 through authentication by microbial taxonomy. The strain is deposited in China center for type culture collection with a deposition date being June-30-2014 and an accession number being CCTCC No: M2014303. The invention has following most significant characteristics: (1) A fermentation liquid of the strain is an active metabolite which has inhibiting effects of sclerotinia sclerotiorum, phytophthora capsici and the like and is an important microbial resource; and (2) A fermentation mycelium of the strain can secret various natural active components, has medicinal effects of brad-spectrum tumor resistance and the like, and is an important microbial resource of a novel natural active drug.

The invention relates to a fungus pestalotiopsis extract and an application thereof and belongs to the field of applied microbiology. The fungus pestalotiopsis extract is obtained through the steps of fungus strain selection and preparation of a pestalotiopsis (Pestalotiopsis sp.) GLGS-5 extract. The fungus strain is pestalotiopsis (Pestalotiopsis sp.) GLGS-5; the strain GLGS-5 is preserved in a Common Microorganism Center of the China Committee for Culture Collection of Microorganisms on January 9, 2017; the preservation unit address is the Institute No.3, of Microbiology of Chinese Academy No.1 of Sciences, West Beichen Road, Chaoyang District of Beijing; and the preservation number is CGMCC No.13577. The fungus pestalotiopsis extract is applied to preparation of an acetylcholin esterase inhibitor. The fungus pestalotiopsis extract has the beneficial effects that the pestalotiopsis (Pestalotiopsis sp.) GLGS-5 solid fermentation extract is good in activity, the inhibition rate on AChE reaches 61.8% and the fungus pestalotiopsis extract can be applied to preparation of the acetylcholin esterase inhibitor.

The invention relates to a heavy metal resistant pestalotiopsis neohirsuta microorganism MCT-13, which is a pestalotiopsis sp. MCT-13 (Neopestalotiopsis sp.MCT-13) and preserved in the China Center for Type Culture Collection and has the preservation number of CCTCC NO M 2020543. The Neopestalotiopsis sp.MCT-13 disclosed by the invention is easy to culture, large in biomass and environment-friendly, and has composite heavy metal resistance and adsorption capacity. Under the condition of heavy metal ions Cr < 6 + >, Cd < 2 + > and Pb < 2 + >, after Neopestalotiopsis sp.MCT-13 and plant straw are co-cultured, a culture product is collected and pyrolyzed to prepare the biochar, and the curing of the heavy metal ions Cr < 6 + >, Cd < 2 + > and Pb < 2 + > can be effectively realized. Neopestalotiopsis sp.MCT-13 and plant straws under the conditions of nitrogen, phosphorus and metal ions Fe < 3 + >, Fe < 2 + >, Mn < 2 + >, Mg < 2 + > and Ca < 2 + >, collecting a culture product, and preparing the biochar composite material by combining a hydrothermal method with a high-temperature pyrolysis method, and the biochar composite material can be applied to heavy metal pollution remediation of water and soil.