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Streptomyces with dual functions of disease prevention and insecticide, application thereof, cultivation method, and biocontrol agent

A dual-action, cultivation method technology, applied in biocides, fungicides, insecticides, etc., can solve problems such as inability to suppress plant pests

Active Publication Date: 2021-04-30
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although some actinomycetes that have antagonistic effects on plant pathogenic bacteria have been reported in the above-mentioned literature, these actinomycetes generally can only antagonize a few kinds of pathogenic bacteria, and cannot inhibit plant pests.

Method used

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  • Streptomyces with dual functions of disease prevention and insecticide, application thereof, cultivation method, and biocontrol agent
  • Streptomyces with dual functions of disease prevention and insecticide, application thereof, cultivation method, and biocontrol agent
  • Streptomyces with dual functions of disease prevention and insecticide, application thereof, cultivation method, and biocontrol agent

Examples

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Embodiment 1

[0040]In this embodiment, the bacterial colony has a double effect of the anti-inwali insecticidosis, and the strain is Streptomycessp.) MS001, the preservation number is CCTCC NO: M 2019266; Warehouse Date: April 17, 2019, Waitsu: China is typical Culture Safe Center, Waitsu, Wuhan University, Wuhan University, Wuhan, Wuchang, Wuchang, Wuhan, Hubei Province, Wuhan University Waition Center).

[0041]Example 1 for the application of Streptomymnasis in antagonistic bacteria with anti-disease insecticidosis

[0042]The strain used in this embodiment is Streptomyces Sp.) MS001, the preservation number is CCTCNO: M2019266. Plant pathogenesis is a rac porphosis, aperture spieropronis, and / or apple stem.

Embodiment 2

[0044]The strain used in this embodiment is Streptomyces Sp.) MS001, the preservation number is CCTCNO: M2019266. Plant pathogen is a wheat ripple, wheat full-etched pathogen, tomato gray bacteria, wheat biblia bacteria, gentlemannoid rotio, and / or South Tianshu anthracis.

[0045]Example 1 for the use of acendomymnasis in inhibiting phytoplasm

[0046]The strain used in this embodiment is Streptomyces Sp.) MS001, the preservation number is CCTCNO: M2019266. Plant pests are cotton bollworms and / or loose brown larvae.

[0047]Example 1 of the cultivation of streptavidic bacteria with anti-disease insecticidosis

[0048]In this example, the culture method having a phenotropic insecticidal double action is used, and the medium used is a high-level medium; cultured conditions: temperature 20-32 ° C, pH = 5-9. More preferably, the culture temperature is 23 ° C, pH = 7.

[0049]Since the cultivated streptomae seed liquid capacity is considered, the amount of fermentation liquid in strain MS001 is 4%...

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Abstract

The invention relates to a streptomycete with dual functions of disease prevention and insecticide, its application, a cultivation method and a biocontrol agent, and belongs to the technical field of plant disease and insect pest control. In the present invention, an endophytic streptomyces MS001 with antibacterial activity is isolated; Botrytis cinerea, wheat scab, clivia stem rot, and nandina anthracnose all have strong antibacterial activity, and the antibacterial objects have a broad spectrum; and other plant pests. Therefore, Streptomyces MS001 can prevent and control some diseases and pests on apple trees, garden plants, vegetables, and food crops.

Description

Technical field[0001]The present invention relates to streptomae and its application, culture method, and biopharmac artifacts having anti-inactive insecticidality, and biopharmaceuticals belong to the field of prevention and treatment of plant pests and diseases.Background technique[0002]Endose bacteria are widely distributed in roots of plants, stems, leaves, fruits and seeds, and a large number of studies have shown great application value from the dispensers separated from plants.[0003]The Australian Research Group has been removed from the wheat root part from 60 strains of the strain, and the strains of Gaeumannomyces graminis var.tritici were screened. The temperature of the greenhouse test was reduced by 70% (Coombs JT, Franco). CM.ISolation and Identification of Actinobacteria fromsurface-Sterilized Wheat Roots [J] .applied and Environmental Microbiology, 2003, 69 (9): 5603 ~ 5608.). Liang Yaping and others separated from wild plants to obtain 29 endogenous amorphous bacter...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/28A01P3/00A01P7/04C12R1/465
CPCC12N1/20A01N63/00C12R2001/465C12N1/205A01N63/10
Inventor 周洲李永丽曲良建王亚红张梦珂常乐
Owner HENAN UNIV OF SCI & TECH
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