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Preparation method of compound and its application

A technology for compounds and microbial strains, applied in the preparation of compounds and their application fields, can solve problems such as high cost and difficulty

Inactive Publication Date: 2012-06-27
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that this compound is an important parent compound for drug research, and the cost and difficulty of chemical synthesis of this compound are relatively high

Method used

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  • Preparation method of compound and its application
  • Preparation method of compound and its application
  • Preparation method of compound and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Compound 1,13-dihydroxy-6-methyl-6,7,8,9,12,13,14,14a-octanehydro-1H-cyclopentane[ f ][1]Separation and preparation of epoxytridecane-4(11aH)-one

[0028] a. The fungus Phomopsis ( Phomopsis chimonanthi ) activation:

[0029] PDA medium: 200 g of potato, 20 g of glucose, 15 g of agar, 1000 mL of water, make a test tube slope, pick mycelium and inoculate it on the test tube slope, 25 o C was cultured for 7 days; the concentration of spores obtained was 1 × 10 6 Individual / mL bacterial suspension;

[0030] b. The fungus Phomopsis ( Phomopsis chimonanthi ) solid fermentation culture:

[0031] Rice medium (preparation process: add 100 g rice and 100 mL water into a 500 mL Erlenmeyer flask, soak overnight, sterilize and cool for later use).

[0032] The prepared bacterial suspension (spore concentration of 1 × 10 6 individual / mL) 5 mL was inoculated on the rice medium, 25 o C cultured for 40 days;

[0033] c. After the fermentation is complete, add ethyl acetate i...

Embodiment 2

[0038] The test of the anti-Aspergillus fumigatus activity of the compound of the present invention is detected by fungi 96 empty microwell plate method antibacterial method

[0039] 1 Preparation of bacterial suspension

[0040] fungus Aspergillus fumigatus Activated on PDB medium for 48 h, so that the bacteria are in the logarithmic growth phase. Take 1–2 single colonies and add them to the corresponding liquid medium, adjust the bacterial concentration so that the bacterial concentration of the fungus is 0.5–2.5×10 4 cfu / mL.

[0041] 2 Sample preparation

[0042] Dissolve the sample with DMSO or water as solvent. If DMSO is used as the solvent, since high concentrations of DMSO may have an inhibitory effect on the test bacteria, it should be diluted with water (generally, the concentration of DMSO should be controlled within 5%).

[0043] 3 add sample

[0044]Take a clean and sterile 96-well cell culture plate. Generally, column A is used as a blank control, and 20 ...

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Abstract

The invention provides a preparation method of the compound 1, 13-dihydroxy-6-methyl-6, 7, 8, 9, 12, 13, 14, 14a-octyl hydrogen-1H-cyclopentane [f][1] epoxytridecane-4(11aH)-one and its application. The compound is obtained by solid fermentation and extraction separation of a production strain, which is wintersweet Phomopsis chimonanthi that is preserved in China general microbiological culture collection center on December 5, 2011, with a preservation number of CGMCC No 5551. The compound prepared in the invention has inhibiting activity on Aspergillus fumigatus.

Description

technical field [0001] The present invention relates to compound 1,13-dihydroxy-6-methyl-6,7,8,9,12,13,14,14a-octane hydrogen-1H-cyclopentane[ f ][1] Preparation method and application of epoxytridecane-4(11aH)-one. Background technique [0002] Natural products are an important source of lead compounds for the discovery of drugs and pesticides. Natural products are some secondary metabolites produced during the growth and development of microorganisms, plants and animals. Among them, some natural products derived from plants have been used as traditional medicines for thousands of years, and some are still used today, such as the most widely used in the world. The widely used analgesic drug Aspirin (Asprin) is derived from the plants of the genus Salix (Salix) and Populus (Populus); and the first anti-cancer drug (Taxol) with sales exceeding 1 billion US dollars was originally derived from the Pacific plant yew. bark. Scientists such as Fleming and Chain’s research on pe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C07D313/00C12P17/08A01N43/22A01P3/00C12R1/645
Inventor 张永刚刘昌衡贾爱荣夏雪奎袁文鹏刘新张绵松孟秀梅
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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