A kind of bacteriostasis method of camphor leaf essential oil
A technology of camphor leaves and essential oils, applied in the directions of essential oils/spice, botanical equipment and methods, fungicides, etc., to achieve the effects of wide selection, low residual environment, and obvious bacteriostatic effect
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Embodiment 1
[0027] The antibacterial activity of the camphor leaf essential oil extracted by the steam distillation method of embodiment 1
[0028] 1) Extraction of camphor leaf essential oil
[0029] Oil camphor leaves are collected from Ji'an, Jiangxi. The essential oil of fresh leaves of camphor camphora was extracted by steam distillation, and the ratio of water to material was 12.5∶1mL g -1 , the extraction power is 1200W, the extraction time is 65min, and the oil yield is 1.64%. It is dried with anhydrous sodium sulfate and stored in a brown bottle, and refrigerated at 4°C for later use.
[0030] 2) Determination of the components of camphor leaf essential oil
[0031] The chromatographic column is Elite-5MS (30m×0.25mm×0.25μm) quartz capillary column. The split ratio was 10:1, and the injection volume was 0.5 μL. Injection port temperature is 280°C, column temperature is 50°C for 2min, at 3°C.min -1 Rise to 180°C, hold for 2min, then increase at 8°C·min -1 Rise to 240°C and k...
Embodiment 2
[0045] Example 2 Simultaneous Distillation and Extraction of Camphor Leaf Essential Oil Antibacterial Activity
[0046] 1) Extraction of camphor leaf essential oil
[0047] Simultaneous distillation and extraction: Weigh 30g of the treated camphor leaf sample, put it in a 500mL round bottom flask, add 300mL of distilled water, install it at one end of the simultaneous distillation extraction device, and heat to a slight boil; the other end of the simultaneous distillation extraction device is filled with 60mL Anhydrous diethyl ether was placed in a 250mL round-bottomed flask, heated with a 40°C constant temperature water bath, and distilled and extracted for 3.5h at the same time, and the obtained extract was washed with anhydrous Na 2SO 4 After drying, the anhydrous ether was removed by a rotary evaporator to obtain the light yellow camphor leaf essential oil with an aromatic smell, and the oil yield was 1.95%. And refrigerated at 4°C for later use.
[0048] 2) Determinati...
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