Phomopsis rhzomorph compound and its preparation method and application
A technology of Phomopsis and compounds, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problem of less symbiotic fungi in seaweed
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Embodiment 1
[0038] A. Medium formula: slant medium formula: take 200g potatoes, peeled, cut into small pieces, add water to boil for 30min, filter with 4 layers of gauze, add 20g glucose, 20g agar to the filtrate, use 50% seawater (seawater and tap water by volume) than mixing) to 1000mL. Seed culture medium formula, add 20g of glucose and 20g of agar to each liter of potato filtrate (preparation method is the same as above), and use 50% seawater (mixture of seawater and tap water by volume) to make up to 1000mL. Fermentation medium formula: same as seed medium.
[0039] B. Fermentation process: slant culture: prepare culture medium according to the above slant culture medium formula, divide into test tubes, sterilize at 121° C. for 20 minutes, and prepare slant culture medium. Pick the Phomopsis sp. hzla01-1 (Phomopsis sp.hzla01-1) and transfer it to the slant medium, cultivate at 28°C for 3 days; prepare the medium according to the formula of the seed medium, sterilize (121°C, 30min), ...
Embodiment 2
[0044] A. Medium formula: slant medium formula: take 300g potatoes, peeled, cut into small pieces, add water to boil for 30min, filter with 4 layers of gauze, add 30g glucose, 30g agar to the filtrate, use 100% seawater (seawater and tap water by volume) than mixing) to 1000mL. Seed culture medium formula, add 30g of glucose and 30g of agar to each liter of potato filtrate (preparation method is the same as above), and use 100% seawater (mixed with tap water by volume) to make the volume to 1000mL. Fermentation medium formula: same as seed medium.
[0045] B. Fermentation process: slant culture: prepare culture medium according to the above slant culture medium formula, divide into test tubes, sterilize at 121° C. for 20 minutes, and prepare slant culture medium. Pick the Phomopsis sp. hzla01-1 (Phomopsis sp.hzla01-1) and transfer it to the slant medium, and cultivate it at 28°C for 5 days; prepare the medium according to the formula of the seed medium, sterilize (121°C, 30mi...
Embodiment 3
[0050] A. Medium formula: slant medium formula: take 150g potatoes, peeled, cut into small pieces, add water to boil for 30min, filter with 4 layers of gauze, add 15g glucose, 15g agar to the filtrate, use 30% seawater (seawater and tap water by volume) than mixing) to 1000mL. Seed medium formula, add 15g glucose and 15g agar to each liter of potato filtrate (preparation method is the same as above), and dilute to 1000mL with 30% seawater (mixed by volume of seawater and tap water). Fermentation medium formula: same as seed medium.
[0051] B. Fermentation process: slant culture: prepare culture medium according to the above slant culture medium formula, divide into test tubes, sterilize at 121° C. for 20 minutes, and prepare slant culture medium. Pick the Phomopsis hzla01-1, homopsis sp.hzla01-1) and transfer it to the slant medium, cultivate at 28°C for 7 days; prepare the medium according to the seed medium formula, sterilize (121°C, 30min), and put the seeds The medium i...
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