Application and preparation of bacillus amyloliquefaciens subsp. plantarum and bacterial agent thereof

Abstract

The invention relates to application and preparation of bacillus amyloliquefaciens subsp. plantarum and a bacterial agent thereof. An XH-9 bacterial strain is identified as bacillus amyloliquefaciens subsp. plantarum according to mycelial morphology, colony characteristics, physiological and biochemical indexes and 16S rDNA sequence analysis and is preserved in the China General Microbiological Culture Collection Center, and a preservation number is CGMCC NO.13151. The strain is high in antagonistic effect on pathogenic fungi such as fusarium oxysporum, bipolaris sorokiniana, fusarium pseudograminearum, colletotrichum gloeosporioides, botryosphaeria dothidea and alternaria alternata and is capable of generating heteroauxin and 1-aminocyclopropanecarboxylic acid (ACC) deaminase to stimulate plant growth and capable of degrading celluloses and can be stably colonized at rhizospheres of crops such as wheat, corn, peppers and the like. The bacterial agent prepared from the bacterial strain can be applied to prevention and treatment of root rot diseases or other soil-borne fungal diseases of crops such as wheat, corn, peppers and the like and has dual functions of disease prevention and growth promotion. The bacterial agent is simple in preparation process, short in fermentation period, low in cost and beneficial to industrial production and transport.

Description

technical field [0001] The invention provides a plant subspecies of Bacillus amyloliquefaciens and the preparation and application of the bacterial agent thereof, belonging to the technical field of agricultural microorganisms. Background technique [0002] Root rot is a general term for root and stem rot diseases caused by fungi, bacteria and nematodes, which can harm hundreds of host plants such as grain, vegetables, cotton, grass and oil crops. In recent years, with the large-scale implementation of continuous cropping, straw returning and no-tillage, the occurrence of root rot has become more and more serious. [0003] Root rot is a soil-borne disease that mainly harms seedlings, and can also occur at the adult plant stage, making it difficult to control. The reasons include: (1) The pathogen is complex. my country has a vast territory, with different crop types, planting seasons and planting systems, as well as different pathogenic types and infection patterns, making...

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Problems solved by technology

my country has a vast territory, with different crop types, planting seasons and planting systems, as well as different pathogen types and infection patterns, making it difficult to control; (2) Lack of resistant varieties

Most of the crop varieties promoted in production are susceptible varieties, with few resistant varieties and difficult breeding; (3) The pathogen is difficult to eradicate

The pathogen can overwinter in the soil or on crop residues, and can survive in the soil for many years. The short-term crop rotation control effect is poor, and long-term crop rotation and fallow are difficult to implement in most areas of my country; (4) It is difficult to identify and diagnose

This kind of disease mainly harms the roots and stem bases of plants, the symptoms are hidden, it is difficult to identify early plant disease, and it is difficult to effectively prevent and control; (5) The research on biological control and ecological regulation mechanism is insufficient, and the development of related products lags behind; (6) Chemical fungicides prevent The effective cycle is short, and the control effect is generally better in the early stage, and the effect is weakened until it disappears in the middle and late stages, and the control effect is not ideal.

[0004] At present, the prevention and control of root rot mainly depends on chemical agents. Long-term use of chemical agents leads to increased resistance of pathogens, poor control effect, and pollutes the environment.

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