67 results about "Burkholderia sp" patented technology

The invention relates to heavy metal resistance plant growth-promoting bacteria and a preparation applying method thereof, which belongs to the bioremediation field of heavy metal polluted environment. A bacteria strain D54 with a preservation number of CGMCC No. 3223 belongs to the Burkholderia sp. and has higher resistance to a plurality of heavy metals, wherein the resistances to Pb <2+>, Cd <2+>, Cu <2+>, Zn <2+> respectively reach 800 mg/L, 1500 mg/L, 150 mg/L and 2500 mg/L. In addition, the bacteria strain D54 has the plant growth-promoting functions of producing plant growth hormone (IAA), producing 1-amino-1-carboxyl cyclopropane (ACC) deaminase, secreting siderophore, dissolving inorganic phosphate, fixing nitrogen and the like, has the biological prevention functions of antagonizing plant pathogenic bacteria inbreak and the like, and can obviously improve the biomass of the plants applied the invention and improve the resistance to diseases and stresses. The number of the effective viable bacteria in liquid preparation reaches 1-2 billion/ml and the number of the effective viable bacteria in solid preparation reaches 1 billion/g. Soaking seeds for 1-2 hours in the liquidpreparation which is diluted 100 times and irrigating the diluted liquid preparation 1-2 times (10ml/kg) after 2-3 weeks of the sprouting of the seeds can effectively improve plant viable bacteria infection probability.

The invention discloses a burkholderia pyrrocinia JK-SH007 which is classified and named as burkholderia pyrrocinia and preserved in China center for type culture collection (CCTCC) with the preservation number of M 209028 and the preservation date of February 18th, 2009. The invention also discloses the application of the burkholderia pyrrocinia in controlling dothiorella gregaria and promoting poplar to grow. The invention further provides a bactericide for controlling dothiorella gregaria, which is obtained by inoculating the JK-SH007 in a culture medium to be fermented and cultured. The JK-SH007 is plant endophyte which has strong inhibiting effect for botryosphaeria dothidea of the poplar and antagonism for various pathogenic fungis, and has the advantages of simple culture condition, easy storage and commercial process as well as good developing and applying prospect.

The invention relates to a Burkholderia sp. and an application thereof. The invention relates to a Burkholderia sp., which is classified and named as Burkholderia sp., and is preserved in China General Microbiological Culture Collection Center with a preservation number of CGMCC No.16428 on September 6, 2018. The application of the Burkholderia sp. in promoting crop growth specifically comprises the step of preparing the Burkholderia sp. into a microbial seed coating agent. The Burkholderia sp. and the application thereof disclosed by the invention have the following beneficial effects: 1, theBurkholderia sp. can promote crop growth and synthesize growth hormones required by plant growth; 2, stable growth of crops can be kept in an acid-base environment within a certain range; 3, phosphorus in the soil can be converted into available phosphorus for plants, so that the planting cost is reduced, and meanwhile, the environment is protected; and 4, the growth of pathogenic bacteria can beinhibited.

The invention discloses burkholderia and an application method thereof. The preservation number of a strain Burkholderia sp. Z-90 is CGMCC No.9970. The strain can produce a large amount of glycolipid biosurfactants in the metabolic process, and the biosurfactants can complex and desorb heavy metals in contaminated soil. The strain is used for treating soil contaminated by heavy metals, the contents of heavy metals such as Zn, Pb, Mn, Cd, Cu and As in the soil are respectively reduced from original 22374.1 mg/kg, 2472.4 mg/kg, 1837.2 mg/kg, 447.0 mg/kg, 709.5 mg/kg and 562.2 mg/kg to 12525.4 mg/kg, 1670.0 mg/kg, 878.1 mg/kg, 278.3 mg/kg, 538.6 mg/kg and 384.3 mg/kg, and the removal rates are respectively 44.0%, 32.5%, 52.2%, 37.7%, 24.1% and 31.6%.

The invention discloses a high-thermal-stability mutant of D-allulose-3-epimerase and an application of the high-thermal-stability mutant of D-allulose-3-epimerase. The 116th bits of serine of the mutant at D-allulose-3-epimerase is mutated to isoleucine, and the 251st bits of lysine is mutated to leucine, and a double mutant enzyme S116I/K251L is obtained. According to the invention, the 116th bit of serine (Ser, S) of D-allulose-3-epimerase BsDPE of Burkholderia sp. MR1 is mutated to isoleucine (Ile, I), and the 251st bits of lysine (Lys, K) is mutated to leucine (Leu, L), thus the thermal stability of the double mutant enzyme S116I/K251L obtained therefrom is significantly improved; and the half-life period at 60 DEG C is improved from the wild type 337.3 min to 417.9 min at present, and is 1.24 times o the wild type; the using cycle of the enzyme in compounding D-allulose is largely prolonged; the high-thermal-stability mutant of D-allulose-3-epimerase has important industrial application value.

The invention belongs to the technical field of environment pollution biological treatment, relates to a microorganism repair technology and in particular relates to a method for enrichment and removal of caesium polluted environmental microorganisms. In the method, heavy metal resisting strain Burkholderia sp.D54 selected and separated from soil polluted by heavy metal is applied to repair of microorganisms polluted by radionuclide caesium. The strain Burkholderia sp.D54 can be grown in a culture medium containing 50mM of caesium; the strain Burkholderia sp.D54 is cultured in a culture medium containing 1mM of caesium for 3 days so that 0.1737+/-0.0017g of thallus by dry weight; and the enrichment amount of the caesium reaches 45+/-3.85mg g<-1> (dry weight thallus), and the removal rate of the caesium in the culture medium is 58.77%. The strain Burkholderia sp.D54 has strong appetency on radionuclide and can be applied to the repair of the environmental microorganisms polluted by the radionuclide caesium, thus the prospect of the strain is wide.

The invention discloses a Burkholderia sp. Strain ES-89 for preventing and treating fungal diseases of plants as well as a culture method and application. The culture method comprises the following steps: A, inoculating an ES-89 strain into a nutrient broth culture liquid for activation; B, inoculating the activated ES-89 into a triangular flask with the nutrient broth culture liquid, and performing shake cultivation; C, centrifuging fermentation broth obtained from shake cultivation under a certain condition, and filtering an upper layer of fermentation broth by a bacterial filter to obtain sterile supernate; D, washing the bacterium with sterile water to prepare a bacterial suspension, and storing the fermentation broth, the fermentation supernate and the bacterial suspension. The strain is preserved with the preservation number of CCTCC, CCTCC NO: M 2016665, and the strain ES-89 can be applied to preparation of medicines for treating or preventing plant pathogenic fungi. The strain has an inhibition function on coptis chinensis leaf spot diseases and the like, and can be applied to prevention and treatment on multiple plant diseases. The prevention and treatment effect of the strain on the coptis chinensis leaf spot diseases can be 88% or greater, the culture method is feasible, the operation is simple and convenient, the pollution in the production process is reduced, solvent poisoning is avoided, and the application prospect is relatively good.

The invention discloses a rice endotrophic azotobacter for producing activated calcium carbonate (ACC) and realizing the antagonistic effect on fusarium graminearum and a purpose thereof. The rice endotrophic azotobacter is Burkholderia sp. GDSD125 and has the preservation number being CGMCC No.5038 in General Microbiology Center of China Microbial Strain Preservation Management Committee. The Burkholderia sp. GDSD125 CGMCC No.5038 provided by the invention has high nitrogen fixation activity and ACC ammonialyase activity, can realize the effective antagonistic effect on gibberella zeae and has wide application prospects in nitrogen fixation microbial agent and microbial organic fertilizer production.

Burkholderia sp. for aerobic degradation of benzpyrole and the application of burkholderia sp. belong to the technical field of microorganism. The strain separated from activated sludge of a reactor is preserved in the Center for General Microorganisms of China Committee for Culture Collection of Microorganisms (CCCCM) on January 28, 2015, with the preservation number of CGMCC No.,10457. 16S Rrna gene sequence determination shows that strain IDO3 and Burkholderia have high similarity, therefore, the strain is classified and named as Burkholderia sp. IDO3, and the accession number of the gen bank of the gene sequence of the strain 16S rRNA is KP895480. The strain has a relatively stronger degradation capacity to benzpyrole, also has excellent Ph and temperature tolerances, and shows the application prospect in treating waste water with high concentration benzpyrole.

The invention discloses a method for forming a biomembrane by using burkholderia pyrrocinia. The method comprises the following steps: 1) preparing single colonies from an LB solid slab activated burkholderia pyrrocinia JK-SH007 strain, selecting the single colonies, putting the single colonies into an LB liquid culture medium, and performing pure culture for 24 hours at 180r/min at 28 DEG C so asto obtain a bacterial suspension; 2) preparing a test culture medium TMG, and adding 1%v/v glycerinum and 25mM of MgSO4 into the TSB (trypticase soy broth) culture medium, wherein the pH value is 5-7; and 3) inoculating the 1%v/v bacterial suspension into the TMG liquid culture medium, performing pure culture for 24 hours at 180r/min at 28 DEG C, taking out the solution, and allowing the solutionto stand for 4-6 days, so as to form the biomembrane. By adopting the method, the JK-SH007 can be induced to form the culture medium TMG of the biomembrane, after culture with the culture medium, theJK-SH007 can form the biomembrane which is visible by naked eyes, and thus the JK-SH007 can survive well in an environment in nutrition deficiency.

The invention belongs to the technical field of microorganism, and particularly relates to a Burkholderia FH4 bacterial strain, and a screening method and application thereof. The preservation number of the Burkholderia FH4 bacterial strain is CGMCC No.8271; the application method of enzymolysis Enteromorpha of the bacterial strain comprises the steps: performing excitation culture on a cryopreserved FH4 bacterial strain in an LB slant culture medium, then selecting and putting a complete ring into an LB culture liquid medium, performing shaking culture for 24 to 28 h, putting 1 to 2 mL of a bacterial solution into cellulose liquid fermentation culture medium subjected to sterilization, performing shaking culture for 5 to 7 days, then performing low-temperature centrifugation on fermentation liquor, using supernate as crude enzyme, or filtering the fermentation liquor, and using filter liquor as crude enzyme. The crude enzyme reacts with clean Enteromorpha powder at 45 to 55 DEG C. The Burkholderia FH4 bacterial strain has the capacity of producing cellulose, can effectively degrade cellulose substance in Enteromorpha into reducing sugar, and is low in production cost, simple in equipment, mild in reaction and applicable to large-scale industrial production.

The invention discloses a Burkholderia sp.HDXY-02 capable of being stably produce toxoflavin with a high yield, and belongs to the technical field of applied microorganisms. The strain is preserved onApril 20, 2017 in China General Microbiological Culture Collection Center (abbreviation CGMCC), and the preservation number is CGMCC No.14054. After the strain is subjected to fermentation in a liquid culture medium or spread plate culture in a solid plate for 48-72 h, extraction is performed by dichloromethane or chloroform, separation purification is performed to obtain the toxoflavin, and thetoxoflavin has the activity of inhibiting pathogenic bacteria aspergillus fumigatus and resisting tumor Lovo cells. The strain provided by the invention can synthesize the toxoflavin by fermentation,and the toxoflavin has a high yield; and compared with a current chemical synthetic method, the strain provided by the invention avoids the pollution to the environment by chemical substances, and haspotential application prospects.

The invention discloses a sophora tonkinensis endophytic bacterium B29. The sophora tonkinensis endophytic bacterium B29 is classified and named as Burkholderia sp. B29, wherein the 16S rDNA gene sequence table of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 13 May 2015, and the depository number is CGMCC No.10807. According to the sophora tonkinensis endophytic bacterium B29 provided by the invention, a strain of endophytic bacterium Burkholderia sp. B29 is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Burkholderia sp. B29 has a strong inhibiting effect on panax notoginseng alternaria panax whetzel, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.

The invention discloses an engineering bacteria strain for heterogenous expression of a histone deacetylases inhibitor Thailandepsin A. The bacterial strain is named as the engineering bacterial strain 7029-tdp-A, which uses Burkholderia sp.DSM7029 as an original strain, and a biosynthetic gene cluster (tdp) of Thailandepsins is integrated on a genome through a transposition mode. The invention also discloses an application of the engineering bacterial strain in preparation of the histone deacetylases inhibitor Thailandepsin A. The experiment proves that the engineering bacterial strain 7029-tdp-A and a wild bacterial strain Burkholderia thailandepsis E264 are compared, the output of Thailandepsin A is increased by one time, and the engineering bacteria strain establishes a base for large-scale preparation and exploitation of the histone deacetylases inhibitor drugs.

The invention discloses a burkholderia strain and application thereof. The strain is named as Burkholderia sp. YCYP9 in taxonomy, and the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M 2021733. The strain has the function of simultaneously dissolving inorganic phosphorus and organic phosphorus, and can be used for preparing a phosphorus-dissolving preparation. Meanwhile, the strain also has a function of promoting the growth of camellia oleifera seedlings, and can be used for preparing a camellia oleifera seedling growth promoter.

The invention discloses a bromoxynil degrading bacterium and application thereof, and belongs to the field of high biotechnologies. A bacterial strain of the bromoxynil degrading bacterium is a Gram staining reaction negative bacterium BX03, and belongs to Burkholderia bacterial genus (Burkholderia sp.) is identified as Burkholderia sp.. The bromoxynil degrading bacterium is mainly biologically characterized in that the bromoxynil degrading bacterium is Gram-negative, is in the shape of a short spar and is free of spores, and flagella grow at the ends of the bromoxynil degrading bacterium. Bromoxynil can be used as the only carbon source and the only nitrogen source to grow the bacterium, and the bacterium can be mineralized. The bromoxynil degrading bacterium and the application have the advantages that 95% of 100mg/L of the bromoxynil can be degraded by the bacterial strain within 24 hours under a flask shaking condition in a laboratory, and accordingly the problem of difficulty in biologically degrading bromoxynil during waste water treatment can be solved.

The invention provides a fermentation strategy capable of improving toxoflavin production of burkholderia sp.HDXY-02, and belongs to the technical field of microbial fermentation. A strain HDXY-02 ispreserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.14054. The strategy determines that the optimal inoculation amount is 3% and the culture temperature is 30 DEG C. Through single factor experiments and statistical analysis, the optimal culture medium conditions are determined as follows: glucose (19.24 g/L), peptone (20.45 g/L), phenylalanine (0.472 g/L), K2HPO4 (1.5 g/L), MgSO4 (0.75 g/L), and pH 7.0. After optimization, the toxoflavin yield reaches 1533 mg/L and is improved by 296% than that before optimization, and the feasible fermentation strategy is provided for the high production of toxoflavin by a microbial fermentation method.